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BMRB: 18434

2LSK

C-terminal domain of human REV1 in complex with DNA-polymerase H (eta)

Authors

Pozhidaeva, A., Pustovalova, Y., Pustovalova, I., Korzhnev, D.

Assembly

C-terminal domain of human REV1 in complex with DNA-polymerase H (eta)

Entity

1. C-terminal domain of human REV1 in complex with DNA-polymerase H (eta), entity 1 (polymer, Thiol state: all free), 95 monomers, 11002.59 Da Detail

GNLAGAVEFN DVKTLLREWI TTISDPMEED ILQVVKYCTD LIEEKDLEKL DLVIKYMKRL MQQSVESVWN MAFDFILDNV QVVLQQTYGS TLKVT

2. C-terminal domain of human REV1 in complex with DNA-polymerase H (eta), entity 2 (polymer, Thiol state: not present), 16 monomers, 1824.080 Da Detail

QSTGTEPFFK QKSLLL

Total weight

12826.67 Da

Max. entity weight

11002.59 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 83.8 %, Completeness: 73.4 %, Completeness (bb): 79.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	73.4 % (993 of 1353)	73.1 % (512 of 700)	72.5 % (384 of 530)	78.9 % (97 of 123)
Backbone	79.2 % (524 of 662)	78.6 % (176 of 224)	79.9 % (263 of 329)	78.0 % (85 of 109)
Sidechain	69.8 % (557 of 798)	70.6 % (336 of 476)	67.9 % (209 of 308)	85.7 % (12 of 14)
Aromatic	24.5 % (24 of 98)	24.5 % (12 of 49)	21.3 % (10 of 47)	100.0 % (2 of 2)
Methyl	81.6 % (124 of 152)	81.6 % (62 of 76)	81.6 % (62 of 76)

1. entity 1

GNLAGAVEFN DVKTLLREWI TTISDPMEED ILQVVKYCTD LIEEKDLEKL DLVIKYMKRL MQQSVESVWN MAFDFILDNV QVVLQQTYGS TLKVT

2. entity 2

QSTGTEPFFK QKSLLL

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 7

#	Name	Isotope labeling	Concentration
1	REV1	[U-99% 13C; U-99% 15N]	0.9 mM
2	DNA-polymerase	natural abundance	0.9 mM
3	sodium phosphate	natural abundance	50 mM
4	sodium chloride	natural abundance	100 mM
5	EDTA	natural abundance	0.25 mM
6	DTT	natural abundance	5 mM
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.0 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.0 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.17 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.2 ppm, Outliers: 4 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2LSK, Strand ID: A, B Detail

Release date

2012-06-17

Citation

NMR structure and dynamics of the C-terminal domain from human Rev1 and its complex with Rev1 interacting region of DNA polymerase η
Pozhidaeva, A., Pustovalova, Y., Bezsonova, S., Walker, I., Korzhnev, G.C.
Biochemistry (2012), 51, 5506-5520, PubMed 22691049 , DOI 10.1021/bi300566z , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 18455 released on 2012-12-17
Title Structure of the C-terminal domain from human REV1

Related entities 1. C-terminal domain of human REV1 in complex with DNA-polymerase H (eta), entity 1, : 3 : 8 entities Detail

Related entities 2. C-terminal domain of human REV1 in complex with DNA-polymerase H (eta), entity 2, : 1 : 2 : 3 entities Detail

Interaction partners 1. C-terminal domain of human REV1 in complex with DNA-polymerase H (eta), entity 1, : 2 : 1 interactors Detail

More details for 3 interactors identified by 2 citations

Interaction partners 2. C-terminal domain of human REV1 in complex with DNA-polymerase H (eta), entity 2, : 7 : 1 interactors Detail

More details for 8 interactors identified by 5 citations

Experiments performed 9 experiments Detail

1 2D 1H-15N HSQC