BMRBj - BMREntryMaster

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BMRB: 18445

2LSU

NMR high resolution structures of free Tah1 and Tah1 bound to the Hsp90 C-terminal tail explain how Hsp90 recognizes the R2TP complex

Authors

BACK, R.R.B., DOMINGUEZ, C., ROTHE, B., BOBO, C., BEAUFILS, C., MORERA, S., MEYER, P., CHARPENTIER, B., BRANLANT, C., ALLAIN, F., MANIVAL, X.

Assembly

yeast Tah1

Entity

1. yeast Tah1 (polymer, Thiol state: all free), 110 monomers, 12379.83 Da Detail

SQFEKQKEQG NSLFKQGLYR EAVHCYDQLI TAQPQNPVGY SNKAMALIKL GEYTQAIQMC QQGLRYTSTA EHVAIRSKLQ YRLELAQGAV GSVQIPVVEV DELPEGYDRS

Formula weight

12379.83 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.2 %, Completeness: 83.7 %, Completeness (bb): 79.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C
All	83.7 % (979 of 1169)	94.8 % (642 of 677)	68.5 % (337 of 492)
Backbone	79.1 % (432 of 546)	96.0 % (215 of 224)	67.4 % (217 of 322)
Sidechain	89.4 % (648 of 725)	94.3 % (427 of 453)	81.3 % (221 of 272)
Aromatic	50.0 % (42 of 84)	100.0 % (42 of 42)	0.0 % (0 of 42)
Methyl	95.8 % (113 of 118)	96.6 % (57 of 59)	94.9 % (56 of 59)

1. entity

SQFEKQKEQG NSLFKQGLYR EAVHCYDQLI TAQPQNPVGY SNKAMALIKL GEYTQAIQMC QQGLRYTSTA EHVAIRSKLQ YRLELAQGAV GSVQIPVVEV DELPEGYDRS

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 288 K, pH 7.2

#	Name	Isotope labeling	Concentration
1	Tah1	[U-100% 13C; U-100% 15N]	1 mM
2	sodium phosphate	natural abundance	10 mM
3	sodium chloride	natural abundance	150 mM
4	H2O	natural abundance	90 %
5	D2O	natural abundance	10 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 288 K, pH 7.2

#	Name	Isotope labeling	Concentration
6	Tah1	[U-100% 13C; U-100% 15N]	1 mM
7	sodium phosphate	natural abundance	10 mM
8	sodium chloride	natural abundance	150 mM
9	D2O	natural abundance	150 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.7 ppm	internal	indirect	0.2514495
¹H	water	protons	4.7 ppm	internal	direct	1.0
¹⁵N	water	protons	4.7 ppm	internal	indirect	0.2514495

Referencing offset: 0.5 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.7 ppm	internal	indirect	0.2514495
¹H	water	protons	4.7 ppm	internal	direct	1.0
¹⁵N	water	protons	4.7 ppm	internal	indirect	0.2514495

Referencing offset: 0.5 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.7 ppm	internal	indirect	0.2514495
¹H	water	protons	4.7 ppm	internal	direct	1.0
¹⁵N	water	protons	4.7 ppm	internal	indirect	0.2514495

Referencing offset: 0.26 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2LSU, Strand ID: A Detail

Release date

2013-05-19

Citation

High-resolution structural analysis shows how Tah1 tethers Hsp90 to the R2TP complex
Back, R., Dominguez, C., Rothe, B., Bobo, C., Beaufils, C., Morera, S., Meyer, P., Charpentier, B., Branlant, C., Allain, F., Manival, X.
Structure (2013), 21, 1834-1847, PubMed 24012479 , DOI 10.1016/j.str.2013.07.024 , Abstract

Related entities 1. yeast Tah1, : 1 : 4 : 2 : 31 entities Detail

Interaction partners 1. yeast Tah1, : 25 interactors Detail

More details for 25 interactors identified by 5 citations

Experiments performed 15 experiments Detail

1 2D 1H-15N HSQC