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BMRB: 18471

2NAS

Backbone 1H, 13C, and 15N Chemical Shift Assignments for PWWP domain from Trypanosoma brucei

Authors

Dai, K., Liao, S., Zhang, J., Tu, X.

Assembly

PWWP domain

Entity

1. PWWP domain (polymer, Thiol state: all free), 118 monomers, 13403.81 Da Detail

MIPSFAPGTL VWLKQDRFPW WPGFVMDPDE VRDITLPEGS DVWVCCLPRD SLTLSAANSE DEGQIRYFLP DRDEGMMEEG KLDASCAVAI EEAIQLYEEQ LKAQAGGTNE LEHHHHHH

Formula weight

13403.81 Da

Source organism

Trypanosoma brucei

Exptl. method

solution NMR

Refine. method

DGSA-distance geometry simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.9 %, Completeness: 70.9 %, Completeness (bb): 76.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	70.9 % (960 of 1354)	78.9 % (554 of 702)	56.9 % (302 of 531)	86.0 % (104 of 121)
Backbone	76.7 % (531 of 692)	92.8 % (219 of 236)	60.7 % (210 of 346)	92.7 % (102 of 110)
Sidechain	67.7 % (523 of 772)	71.5 % (333 of 466)	64.1 % (189 of 295)	9.1 % (1 of 11)
Aromatic	0.0 % (0 of 128)	0.0 % (0 of 64)	0.0 % (0 of 60)	0.0 % (0 of 4)
Methyl	80.7 % (92 of 114)	86.0 % (49 of 57)	75.4 % (43 of 57)

1. PWWP domain

MIPSFAPGTL VWLKQDRFPW WPGFVMDPDE VRDITLPEGS DVWVCCLPRD SLTLSAANSE DEGQIRYFLP DRDEGMMEEG KLDASCAVAI EEAIQLYEEQ LKAQAGGTNE LEHHHHHH

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	PWWP_domain	[U-99% 13C; U-99% 15N]	0.6 mM
2	sodium phosphate	natural abundance	25 mM
3	sodium chloride	natural abundance	100 mM
4	DTT	natural abundance	1 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 3.04 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 3.04 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.05 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2NAS, Strand ID: A Detail

Release date

2012-08-28

Citation

¹H, ¹³C and ¹³N resonance assignments of TbTFIIS2-2 PWWP domain from Trypanosoma brucei
Zhang, J., Dai, K., Liao, S., Tu, X.
Biomol. NMR Assign. (2013), 7, 207-209, PubMed 22836947 , DOI 10.1007/s12104-012-9411-0 , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 18474 released on 2012-08-28
Title the pwwp domain of TFIIS2-1 from Trypanosoma brucei

Related entities 1. PWWP domain, : 15 entities Detail

Experiments performed 7 experiments Detail

1 2D 1H-15N HSQC