BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	81.1 % (625 of 771)	88.1 % (342 of 388)	69.1 % (215 of 311)	94.4 % (68 of 72)
Backbone	80.4 % (336 of 418)	92.4 % (133 of 144)	66.2 % (137 of 207)	98.5 % (66 of 67)
Sidechain	83.7 % (350 of 418)	85.7 % (209 of 244)	82.2 % (139 of 169)	40.0 % (2 of 5)
Aromatic	44.0 % (22 of 50)	64.0 % (16 of 25)	20.8 % (5 of 24)	100.0 % (1 of 1)
Methyl	94.4 % (102 of 108)	92.6 % (50 of 54)	96.3 % (52 of 54)

1. P2 gpX

MKTFALQGDT LDAICVRYYG RTEGVVETVL AANPGLAELG AVLPHGTAVE LPDVQTAPVA ETVNLWEVEH H

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	P2_gpX	[U-100% 13C; U-100% 15N]	1.1 mM
2	sodium phosphate	natural abundance	25 mM
3	sodium chloride	natural abundance	200 mM
4	DTT	natural abundance	2 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 273 K, pH 6.8

#	Name	Isotope labeling	Concentration
7	P2 gpX	[U-100% 13C; U-100% 15N]	1.1 mM
8	sodium phosphate	natural abundance	25 mM
9	sodium chloride	natural abundance	200 mM
10	DTT	natural abundance	2 mM
11	D2O	natural abundance	100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.11 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.11 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.01 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2LTF, Strand ID: A Detail

Release date

2013-05-19

Citation

Structural and functional studies of gpX of Escherichia coli phage P2 reveal a widespread role for LysM domains in the baseplates of contractile-tailed phages
Maxwell, K.L., Fatehi Hassanabad, M., Chang, T., Pirani, N., Bona, D., Edwards, A.M., Davidson, A.R.
J. Bacteriol. (2013), 195, 5461-5468, PubMed 24097944 , DOI 10.1128/JB.00805-13 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Swiss-Prot: P08557 released on 1988-08-01
Title CIRCN_BPMU Entity DNA circularization protein N

Related entities 1. P2 gpX, : 1 : 1 : 4 entities Detail

Experiments performed 14 experiments Detail

1 3D HNCACB

Instrument

Varian INOVA - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	P2_gpX	[U-100% 13C; U-100% 15N]	1.1 mM
2	sodium phosphate	natural abundance	25 mM
3	sodium chloride	natural abundance	200 mM
4	DTT	natural abundance	2 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

2 3D CBCA(CO)NH

Instrument

Varian INOVA - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	P2_gpX	[U-100% 13C; U-100% 15N]	1.1 mM
2	sodium phosphate	natural abundance	25 mM
3	sodium chloride	natural abundance	200 mM
4	DTT	natural abundance	2 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

3 3D HNCO

Instrument

Varian INOVA - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	P2_gpX	[U-100% 13C; U-100% 15N]	1.1 mM
2	sodium phosphate	natural abundance	25 mM
3	sodium chloride	natural abundance	200 mM
4	DTT	natural abundance	2 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

4 2D 1H-15N HSQC

Instrument

Varian INOVA - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	P2_gpX	[U-100% 13C; U-100% 15N]	1.1 mM
2	sodium phosphate	natural abundance	25 mM
3	sodium chloride	natural abundance	200 mM
4	DTT	natural abundance	2 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

5 3D 1H-15N NOESY

Instrument

Varian INOVA - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	P2_gpX	[U-100% 13C; U-100% 15N]	1.1 mM
2	sodium phosphate	natural abundance	25 mM
3	sodium chloride	natural abundance	200 mM
4	DTT	natural abundance	2 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

6 3D H(CCO)NH

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	P2_gpX	[U-100% 13C; U-100% 15N]	1.1 mM
2	sodium phosphate	natural abundance	25 mM
3	sodium chloride	natural abundance	200 mM
4	DTT	natural abundance	2 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

7 3D C(CO)NH

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	P2_gpX	[U-100% 13C; U-100% 15N]	1.1 mM
2	sodium phosphate	natural abundance	25 mM
3	sodium chloride	natural abundance	200 mM
4	DTT	natural abundance	2 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

8 2D 1H-13C HSQC

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	P2_gpX	[U-100% 13C; U-100% 15N]	1.1 mM
2	sodium phosphate	natural abundance	25 mM
3	sodium chloride	natural abundance	200 mM
4	DTT	natural abundance	2 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

9 3D 1H-13C NOESY aliphatic

Instrument

Varian INOVA - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 273 K, pH 6.8

#	Name	Isotope labeling	Concentration
7	P2 gpX	[U-100% 13C; U-100% 15N]	1.1 mM
8	sodium phosphate	natural abundance	25 mM
9	sodium chloride	natural abundance	200 mM
10	DTT	natural abundance	2 mM
11	D2O	natural abundance	100 %

10 2D 1H-13C HSQC aliphatic

Instrument

Varian INOVA - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 273 K, pH 6.8

#	Name	Isotope labeling	Concentration
7	P2 gpX	[U-100% 13C; U-100% 15N]	1.1 mM
8	sodium phosphate	natural abundance	25 mM
9	sodium chloride	natural abundance	200 mM
10	DTT	natural abundance	2 mM
11	D2O	natural abundance	100 %

11 3D 1H-13C NOESY aromatic

Instrument

Varian INOVA - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 273 K, pH 6.8

#	Name	Isotope labeling	Concentration
7	P2 gpX	[U-100% 13C; U-100% 15N]	1.1 mM
8	sodium phosphate	natural abundance	25 mM
9	sodium chloride	natural abundance	200 mM
10	DTT	natural abundance	2 mM
11	D2O	natural abundance	100 %

12 2D 1H-13C HSQC aromatic

Instrument

Varian INOVA - 800 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 273 K, pH 6.8

#	Name	Isotope labeling	Concentration
7	P2 gpX	[U-100% 13C; U-100% 15N]	1.1 mM
8	sodium phosphate	natural abundance	25 mM
9	sodium chloride	natural abundance	200 mM
10	DTT	natural abundance	2 mM
11	D2O	natural abundance	100 %

13 3D 1H-13C NOESY aliphatic

Instrument

Varian INOVA - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	P2_gpX	[U-100% 13C; U-100% 15N]	1.1 mM
2	sodium phosphate	natural abundance	25 mM
3	sodium chloride	natural abundance	200 mM
4	DTT	natural abundance	2 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

14 3D HCCH-TOCSY

Instrument

Varian INOVA - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.8

#	Name	Isotope labeling	Concentration
1	P2_gpX	[U-100% 13C; U-100% 15N]	1.1 mM
2	sodium phosphate	natural abundance	25 mM
3	sodium chloride	natural abundance	200 mM
4	DTT	natural abundance	2 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

NMR combined restraints 3 contents Detail

Properties

Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints	combined_18475_2ltf.nef
Input source #2: Coordindates	2ltf.cif
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Sequence alignments

Entity_assembly_ID (NMR): A vs Auth_asym_ID (model): A

--------10--------20--------30--------40--------50--------60--------70-
MKTFALQGDTLDAICVRYYGRTEGVVETVLAANPGLAELGAVLPHGTAVELPDVQTAPVAETVNLWEVEHH
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
MKTFALQGDTLDAICVRYYGRTEGVVETVLAANPGLAELGAVLPHGTAVELPDVQTAPVAETVNLWEVEHH

Chain assignments

Entity_assembly_ID (NMR)	Auth_asym_ID (model)	Length	Unmapped	Conflict	Sequence coverage (%)
A	A	71	0	0	100.0

Content subtype: combined_18475_2ltf.nef

#	Content subtype	Saveframe	Status	# of rows (sets)	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	635		100.0 (chain: A, length: 71)
1	Distance restraints	DYANA/DIANA_distance_constraints_2	Warning	1569 (1)	noe	98.6 (chain: A, length: 71)

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 71, Sequence coverage: 100.0%
- Total number of assignments
  - ¹H chemical shifts: 359
  - ¹³C chemical shifts: 208
  - ¹⁵N chemical shifts: 68
- Completeness of assignments
  - Entity_assemble_ID: A
- Redox state of CYS
- Peptide bond of PRO
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: A

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	388	358	92.3
¹³C chemical shifts	311	208	66.9
¹⁵N chemical shifts	74	68	91.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	144	140	97.2
¹³C chemical shifts	142	69	48.6
¹⁵N chemical shifts	67	66	98.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	244	218	89.3
¹³C chemical shifts	169	139	82.2
¹⁵N chemical shifts	7	2	28.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	55	52	94.5
¹³C chemical shifts	55	49	89.1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	25	16	64.0
¹³C chemical shifts	24	5	20.8
¹⁵N chemical shifts	1	1	100.0

Distance restraints

Saveframe: DYANA/DIANA_distance_constraints_2
- Status: Warning
- Experiment type: noe
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 71, Sequence coverage: 98.6%
- Total number of restraints: 1569
- Weight of restraints: 1.0 (1569)
- Potential type of restraints: upper-bound-parabolic (1569)
- Range of distance target values: 2.55, 5.5 (Å)
- Histogram of distance target values
- Histogram of discrepancy in distance target values
- Distance restraints per residue
  - Chain_ID: A
- Distance restraints on contact map

Keywords LysM, Phage P2, phage tail

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Found 1 Document (0.799 seconds)

BMRB: 18475

Sample #1

Sample #2

Chemical shift reference

Chemical shift reference

Chemical shift reference

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. P2 gpX, : 1 : 1 : 4 entities Detail

Experiments performed 14 experiments Detail

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NMR combined restraints 3 contents Detail