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BMRB: 18478

2M16

P75/LEDGF PWWP Domain

Authors

Crowe, B.L., Foster, M.P.

Assembly

P75/LEDGF PWWP Domain

Entity

1. P75/LEDGF PWWP Domain (polymer, Thiol state: not present), 97 monomers, 11013.44 Da Detail

GPGSMTRDFK PGDLIFAKMK GYPHWPARVD EVPDGAVKPP TNKLPIFFFG THETAFLGPK DIFPYSENKE KYGKPNKRKG FNEGLWEIDN NPKVKFS

Formula weight

11013.44 Da

Source organism

Exptl. method

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.9 %, Completeness: 69.4 %, Completeness (bb): 92.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	69.4 % (829 of 1194)	65.9 % (418 of 634)	70.7 % (330 of 467)	87.1 % (81 of 93)
Backbone	92.1 % (514 of 558)	91.1 % (175 of 192)	91.8 % (258 of 281)	95.3 % (81 of 85)
Sidechain	55.5 % (401 of 723)	55.4 % (245 of 442)	57.1 % (156 of 273)	0.0 % (0 of 8)
Aromatic	1.4 % (2 of 146)	2.7 % (2 of 73)	0.0 % (0 of 71)	0.0 % (0 of 2)
Methyl	93.8 % (60 of 64)	93.8 % (30 of 32)	93.8 % (30 of 32)

1. entity

GPGSMTRDFK PGDLIFAKMK GYPHWPARVD EVPDGAVKPP TNKLPIFFFG THETAFLGPK DIFPYSENKE KYGKPNKRKG FNEGLWEIDN NPKVKFS

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	entity	[U-99% 13C; U-99% 15N]		0.35 ~ 0.45 mM
2	HEPES	natural abundance		50 mM
3	sodium chloride	natural abundance		150 mM
4	beta-mercaptoethanol	natural abundance		2 mM
5	DSS	natural abundance		0.66 mM
6	H2O	natural abundance		95 %
7	D2O	natural abundance		5 %

LACS Plot; CA

Referencing offset: 0.02 ppm, Outliers: 2 Detail

LACS Plot; CB

Referencing offset: 0.02 ppm, Outliers: 2 Detail

LACS Plot; HA

Referencing offset: 0.02 ppm, Outliers: 1 Detail

LACS Plot; CO

Referencing offset: 0.67 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2M16, Strand ID: A Detail

Release date

2013-02-26

Citation

Structural basis for high-affinity binding of LEDGF PWWP to mononucleosomes
Eidahl, J.O., Crowe, B.L., North, J.A., McKee, C.J., Shkriabai, N., Feng, L., Plumb, M., Graham, R.L., Gorelick, R.J., Hess, S., Poirier, M.G., Foster, M.P., Kvaratskhelia, M.
Nucleic Acids Res. (2013), 41, 3924-3936, PubMed 23396443 , DOI 10.1093/nar/gkt074 , Abstract

Related entities 1. P75/LEDGF PWWP Domain, : 1 : 11 : 71 entities Detail

Interaction partners 1. P75/LEDGF PWWP Domain, : 27 interactors Detail

More details for 27 interactors identified by 16 citations

Experiments performed 12 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	entity	[U-99% 13C; U-99% 15N]		0.35 ~ 0.45 mM
2	HEPES	natural abundance		50 mM
3	sodium chloride	natural abundance		150 mM
4	beta-mercaptoethanol	natural abundance		2 mM
5	DSS	natural abundance		0.66 mM
6	H2O	natural abundance		95 %
7	D2O	natural abundance		5 %

2 2D 1H-13C HSQC aromatic

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	entity	[U-99% 13C; U-99% 15N]		0.35 ~ 0.45 mM
2	HEPES	natural abundance		50 mM
3	sodium chloride	natural abundance		150 mM
4	beta-mercaptoethanol	natural abundance		2 mM
5	DSS	natural abundance		0.66 mM
6	H2O	natural abundance		95 %
7	D2O	natural abundance		5 %

3 2D 1H-13C HSQC aliphatic

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	entity	[U-99% 13C; U-99% 15N]		0.35 ~ 0.45 mM
2	HEPES	natural abundance		50 mM
3	sodium chloride	natural abundance		150 mM
4	beta-mercaptoethanol	natural abundance		2 mM
5	DSS	natural abundance		0.66 mM
6	H2O	natural abundance		95 %
7	D2O	natural abundance		5 %

4 3D HNCO

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	entity	[U-99% 13C; U-99% 15N]		0.35 ~ 0.45 mM
2	HEPES	natural abundance		50 mM
3	sodium chloride	natural abundance		150 mM
4	beta-mercaptoethanol	natural abundance		2 mM
5	DSS	natural abundance		0.66 mM
6	H2O	natural abundance		95 %
7	D2O	natural abundance		5 %

5 3D CBCA(CO)NH

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	entity	[U-99% 13C; U-99% 15N]		0.35 ~ 0.45 mM
2	HEPES	natural abundance		50 mM
3	sodium chloride	natural abundance		150 mM
4	beta-mercaptoethanol	natural abundance		2 mM
5	DSS	natural abundance		0.66 mM
6	H2O	natural abundance		95 %
7	D2O	natural abundance		5 %

6 3D HNCACB

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	entity	[U-99% 13C; U-99% 15N]		0.35 ~ 0.45 mM
2	HEPES	natural abundance		50 mM
3	sodium chloride	natural abundance		150 mM
4	beta-mercaptoethanol	natural abundance		2 mM
5	DSS	natural abundance		0.66 mM
6	H2O	natural abundance		95 %
7	D2O	natural abundance		5 %

7 3D HBHA(CO)NH

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	entity	[U-99% 13C; U-99% 15N]		0.35 ~ 0.45 mM
2	HEPES	natural abundance		50 mM
3	sodium chloride	natural abundance		150 mM
4	beta-mercaptoethanol	natural abundance		2 mM
5	DSS	natural abundance		0.66 mM
6	H2O	natural abundance		95 %
7	D2O	natural abundance		5 %

8 3D (H)CC(CO)NH-TOCSY

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	entity	[U-99% 13C; U-99% 15N]		0.35 ~ 0.45 mM
2	HEPES	natural abundance		50 mM
3	sodium chloride	natural abundance		150 mM
4	beta-mercaptoethanol	natural abundance		2 mM
5	DSS	natural abundance		0.66 mM
6	H2O	natural abundance		95 %
7	D2O	natural abundance		5 %

9 3D HCCH-TOCSY

Instrument

Bruker DRX - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	entity	[U-99% 13C; U-99% 15N]		0.35 ~ 0.45 mM
2	HEPES	natural abundance		50 mM
3	sodium chloride	natural abundance		150 mM
4	beta-mercaptoethanol	natural abundance		2 mM
5	DSS	natural abundance		0.66 mM
6	H2O	natural abundance		95 %
7	D2O	natural abundance		5 %

10 3D 1H-15N NOESY

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	entity	[U-99% 13C; U-99% 15N]		0.35 ~ 0.45 mM
2	HEPES	natural abundance		50 mM
3	sodium chloride	natural abundance		150 mM
4	beta-mercaptoethanol	natural abundance		2 mM
5	DSS	natural abundance		0.66 mM
6	H2O	natural abundance		95 %
7	D2O	natural abundance		5 %

11 3D 1H-13C NOESY aliphatic

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	entity	[U-99% 13C; U-99% 15N]		0.35 ~ 0.45 mM
2	HEPES	natural abundance		50 mM
3	sodium chloride	natural abundance		150 mM
4	beta-mercaptoethanol	natural abundance		2 mM
5	DSS	natural abundance		0.66 mM
6	H2O	natural abundance		95 %
7	D2O	natural abundance		5 %

12 3D 1H-13C NOESY aromatic

Instrument

Bruker DRX - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5

#	Name	Isotope labeling	Type	Concentration
1	entity	[U-99% 13C; U-99% 15N]		0.35 ~ 0.45 mM
2	HEPES	natural abundance		50 mM
3	sodium chloride	natural abundance		150 mM
4	beta-mercaptoethanol	natural abundance		2 mM
5	DSS	natural abundance		0.66 mM
6	H2O	natural abundance		95 %
7	D2O	natural abundance		5 %

nullKeywords protein, PWWP domain