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BMRB: 18490

2LTO

TDRD3 complex

Authors

Sikorsky, T.

Assembly

TDRD3 complex

Entity

1. TDRD3 complex, entity 1 (polymer, Thiol state: all free), 58 monomers, 6820.748 Da Detail

MKMWKPGDEC FALYWEDNKF YRAEVEALHS SGMTAVVKFI DYGNYEEVLL SNIKPILE

2. TDRD3 2 (polymer, Thiol state: not present), 13 monomers, 1494.604 Da Detail

YSPSSPXYTP QSP

Total weight

8315.352 Da

Max. entity weight

6820.748 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 80.3 %, Completeness: 61.9 %, Completeness (bb): 63.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	61.9 % (523 of 845)	66.1 % (291 of 440)	53.4 % (179 of 335)	75.7 % (53 of 70)
Backbone	63.5 % (259 of 408)	78.1 % (107 of 137)	48.8 % (101 of 207)	79.7 % (51 of 64)
Sidechain	61.9 % (312 of 504)	60.7 % (184 of 303)	64.6 % (126 of 195)	33.3 % (2 of 6)
Aromatic	70.8 % (75 of 106)	71.7 % (38 of 53)	68.6 % (35 of 51)	100.0 % (2 of 2)
Methyl	90.0 % (54 of 60)	90.0 % (27 of 30)	90.0 % (27 of 30)

1. entity 1

MKMWKPGDEC FALYWEDNKF YRAEVEALHS SGMTAVVKFI DYGNYEEVLL SNIKPILE

2. TDRD3 2

YSPSSPXYTP QSP

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293.15 K, pH 8

#	Name	Isotope labeling	Concentration
1	TDRD3_1	[U-99% 13C; U-99% 15N]	1.7 mM
2	sodium phosphate	natural abundance	50 mM
3	beta-mercaptoethanol	natural abundance	10 mM
4	sodium chloride	natural abundance	150 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	TMS	methyl carbons	0.0 ppm	external	direct	1.0
¹H	water	protons	4.7 ppm	internal	direct	1.0
¹⁵N	nitromethane	nitrogen	0.0 ppm	external	direct	1.0

Referencing offset: 2.52 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	TMS	methyl carbons	0.0 ppm	external	direct	1.0
¹H	water	protons	4.7 ppm	internal	direct	1.0
¹⁵N	nitromethane	nitrogen	0.0 ppm	external	direct	1.0

Referencing offset: 2.52 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	TMS	methyl carbons	0.0 ppm	external	direct	1.0
¹H	water	protons	4.7 ppm	internal	direct	1.0
¹⁵N	nitromethane	nitrogen	0.0 ppm	external	direct	1.0

Referencing offset: 0.04 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2LTO, Strand ID: A, B Detail

Release date

2012-11-01

Citation

Recognition of asymmetrically dimethylated arginine by TDRD3
Sikorsky, T., Hobor, F., Krizanova, E., Pasulka, J., Kubicek, K., Stefl, R.
Nucleic Acids Res. (2012), 40, 11748-11755, PubMed 23066109 , DOI 10.1093/nar/gks929 , Abstract

Related entities 1. TDRD3 complex, entity 1, : 2 : 135 entities Detail

Related entities 2. TDRD3 2, : 1 : 304 entities Detail

Interaction partners 1. TDRD3 complex, entity 1, : 13 interactors Detail

More details for 13 interactors identified by 4 citations

Experiments performed 6 experiments Detail

1 2D 1H-15N HSQC