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BMRB: 18496

2LTT

Solution NMR Structure of YdbC:dT19G1 complex. Northeast Structural Genomics Consortium (NESG) Target KR150

Authors

Rossi, P., Barbieri, C.M., Aramini, J.A., Bini, E., Lee, H., Janjua, H., Ciccosanti, C., Wang, H., Acton, T.B., Xiao, R., Everett, J.K., Montelione, G.T.

Assembly

YdbC:dT19G1 complex

Entity

1. YdbC:dT19G1 complex, entity 1 (polymer, Thiol state: not present), 74 monomers, 8646.765 × 2 Da Detail

MADKLKFEII EELIVLSENA KGWRKELNRV SWNDAEPKYD IRTWSPDHEK MGKGITLSEE EFGVLLKELG NKLE

2. DNA (5'-D(*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*T)-3') (polymer), 14 monomers, 4276.713 Da Detail

TTTTTTTTTT TTTT

Total weight

21570.242 Da

Max. entity weight

8646.765 Da

Source organism

Lactococcus lactis

Exptl. method

NMR

Refine. method

restrained molecular dynamics

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 98.9 %, Completeness: 89.4 %, Completeness (bb): 87.9 % Detail

Polymer type: polypeptide(L) polydeoxyribonucleotide

	Total	¹H	¹³C	¹⁵N
All	89.4 % (939 of 1050)	87.6 % (543 of 620)	93.2 % (327 of 351)	87.3 % (69 of 79)
Backbone	87.9 % (473 of 538)	83.9 % (209 of 249)	92.2 % (200 of 217)	88.9 % (64 of 72)
Sidechain	91.9 % (534 of 581)	90.0 % (334 of 371)	96.1 % (195 of 203)	71.4 % (5 of 7)
Aromatic	85.4 % (82 of 96)	77.4 % (48 of 62)	100.0 % (31 of 31)	100.0 % (3 of 3)
Methyl	100.0 % (92 of 92)	100.0 % (53 of 53)	100.0 % (39 of 39)

1. entity 1

MADKLKFEII EELIVLSENA KGWRKELNRV SWNDAEPKYD IRTWSPDHEK MGKGITLSEE EFGVLLKELG NKLE

2. DNA (5'-D(*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*T)-3')

TTTTTTTTTT TTTT

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5, Details 0.6 mM KR150.020, 0.6mM dT19G1 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	KR150.020	[U-100% 13C; U-100% 15N]	0.6 mM
2	NaN3	natural abundance	0.02 %
3	DTT	natural abundance	10 mM
4	NaCL	natural abundance	100 mM
5	D2O	natural abundance	10 %
6	DSS	natural abundance	50 uM
7	TRIS-HCl	natural abundance	10 mM
8	DNA chain	natural abundance	0.3 mM
9	H2O	natural abundance	90 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.17 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.17 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.14 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.08 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2LTT, Strand ID: A, B Detail

Release date

2012-06-13

Related entities 1. YdbC:dT19G1 complex, entity 1, : 1 : 1 : 7 entities Detail

Experiments performed 13 experiments Detail

1 2D 1H-15N HSQC