BMRB: 18497

Solution Structure of autoinhibitory domain of human AMP-activated protein kinase catalytic subunit

Authors

Xia, B., Hu, J.

Assembly

autoinhibitory domain of human AMP-activated protein kinase catalytic subunit

Entity

1. autoinhibitory domain of human AMP-activated protein kinase catalytic subunit (polymer), 62 monomers, 7020.666 Da Detail

GPHMSYDANV IDDEAVKEVC EKFECTESEV MNSLYSGDPQ DQLAVAYHLI IDNRRIMNQA SE

Formula weight

7020.666 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.4 %, Completeness: 86.1 %, Completeness (bb): 97.6 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	86.1 % (597 of 693)	84.7 % (304 of 359)	86.5 % (231 of 267)	92.5 % (62 of 67)
Backbone	97.6 % (359 of 368)	97.6 % (121 of 124)	97.3 % (179 of 184)	98.3 % (59 of 60)
Sidechain	77.4 % (298 of 385)	77.9 % (183 of 235)	78.3 % (112 of 143)	42.9 % (3 of 7)
Aromatic	9.5 % (4 of 42)	19.0 % (4 of 21)	0.0 % (0 of 21)
Methyl	96.7 % (58 of 60)	96.7 % (29 of 30)	96.7 % (29 of 30)

1. entity

GPHMSYDANV IDDEAVKEVC EKFECTESEV MNSLYSGDPQ DQLAVAYHLI IDNRRIMNQA SE

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0, Details 15N/13C labeled AID protein

#	Name	Isotope labeling	Type	Concentration
1	AID protein	[U-100% 13C; U-100% 15N]		0.2 mM
2	sodium phosphate	natural abundance		50 mM
3	D2O	[U-100% 2H]		10 %
4	H2O	natural abundance		90 %
5	EDTA	natural abundance		1 mM

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LACS Plot; CA

Referencing offset: 0.04 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.04 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.02 ppm, Outliers: 3 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CO

Referencing offset: -0.15 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2LTU, Strand ID: A Detail

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Release date

2013-06-09

Citation

Solution Structure of autoinhibitory domain of human AMP-activated protein kinase catalytic subunit
Xia, B., Hu, J.
Not known

Related entities 1. autoinhibitory domain of human AMP-activated protein kinase catalytic subunit, : 1 : 14 : 51 entities Detail

Interaction partners 1. autoinhibitory domain of human AMP-activated protein kinase catalytic subunit, : 51 interactors Detail

Experiments performed 14 experiments Detail