Solution NMR structure of the apo-form of the beta2 carbohydrate module of AMP-activated protein kinase
GAMGIRNSDS VKPTQQARPT VIRWSEGGKE VFISGSFNNW STKIPLIKSH NDFVAILDLP EGEHQYKFFV DGQWVHDPSE PVVTSQLGTI NNLIHVKKSD FEVFD
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 88.9 % (1105 of 1243) | 92.7 % (598 of 645) | 82.9 % (402 of 485) | 92.9 % (105 of 113) |
Backbone | 96.0 % (593 of 618) | 96.2 % (204 of 212) | 96.1 % (295 of 307) | 94.9 % (94 of 99) |
Sidechain | 84.2 % (608 of 722) | 91.0 % (394 of 433) | 73.8 % (203 of 275) | 78.6 % (11 of 14) |
Aromatic | 47.7 % (62 of 130) | 87.7 % (57 of 65) | 3.2 % (2 of 62) | 100.0 % (3 of 3) |
Methyl | 93.5 % (101 of 108) | 94.4 % (51 of 54) | 92.6 % (50 of 54) |
1. entity
GAMGIRNSDS VKPTQQARPT VIRWSEGGKE VFISGSFNNW STKIPLIKSH NDFVAILDLP EGEHQYKFFV DGQWVHDPSE PVVTSQLGTI NNLIHVKKSD FEVFDSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.9
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | entity | [U-98% 15N] | 0.7 (±0.1) mM | |
2 | sodium phosphate | natural abundance | 50 mM | |
3 | sodium azide | natural abundance | .02 % | |
4 | H2O | natural abundance | 90 % | |
5 | D2O | natural abundance | 10 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.9
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
6 | entity | [U-98% 13C; U-98% 15N] | 0.7 (±0.1) mM | |
7 | sodium phosphate | natural abundance | 50 mM | |
8 | sodium azide | natural abundance | .02 % | |
9 | H2O | natural abundance | 90 % | |
10 | D2O | natural abundance | 10 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.9
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
11 | entity | natural abundance | 0.7 (±0.1) mM | |
12 | sodium phosphate | natural abundance | 50 mM | |
13 | sodium azide | natural abundance | .02 % | |
14 | D2O | natural abundance | 100 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.9
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
15 | entity | [U-98% 13C; U-98% 15N] | 0.7 (±0.1) mM | |
16 | sodium phosphate | natural abundance | 50 mM | |
17 | sodium azide | natural abundance | 0.02 % | |
18 | D2O | natural abundance | 100 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.9
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
19 | entity | [U-10% 13C] | 0.7 (±0.1) mM | |
20 | sodium phosphate | natural abundance | 50 mM | |
21 | sodium azide | natural abundance | 0.02 % | |
22 | D2O | natural abundance | 100 % |