BMRB: 18513

RECOMBINANT TAMAPIN NMR solution structure.

Authors

del Rio-Portilla, F., Ramirez-Cordero, B.

Assembly

recombinant tamapin

Entity

1. recombinant tamapin (polymer, Thiol state: all disulfide bound), 31 monomers, 3465.144 Da Detail

AFCNLRRCEL SCRSLGLLGK CIGEECKCVP Y

Formula weight

3465.144 Da

Entity Connection

disulfide 3 Detail

---

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS3:SG	1:CYS21:SG
2	disulfide	sing	1:CYS8:SG	1:CYS26:SG
3	disulfide	sing	1:CYS12:SG	1:CYS28:SG

Source organism

Mesobuthus tamulus

Exptl. method

solution NMR

Refine. method

molecular dynamics, simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 97.3 %, Completeness (bb): 98.4 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H
All	97.3 % (181 of 186)	97.3 % (181 of 186)
Backbone	98.4 % (63 of 64)	98.4 % (63 of 64)
Sidechain	96.7 % (118 of 122)	96.7 % (118 of 122)
Aromatic	88.9 % (8 of 9)	88.9 % (8 of 9)
Methyl	80.0 % (12 of 15)	80.0 % (12 of 15)

1. recombinant-tamapin

AFCNLRRCEL SCRSLGLLGK CIGEECKCVP Y

Show sample condition

Sample

Solvent system 95% H2O, 5% D2O ), Pressure 1 atm, Temperature 298 K, pH 6.0, Details buffer NA

#	Name	Isotope labeling	Type	Concentration
1	recombinant-tamapin	natural abundance		0.8 mM
2	H2O	natural abundance		95 %
3	D2O	natural abundance		5 %

Hide sample codition

Protein Blocks Logo

Calculated from 18 models in PDB: 2LU9, Strand ID: A Detail

---

Release date

2013-12-08

Related entities 1. recombinant tamapin, : 1 : 3 : 6 : 10 : 42 entities Detail

Experiments performed 3 experiments Detail