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BMRB: 18523

4AXP

NMR structure of Hsp12, a protein induced by and required for dietary restriction-induced lifespan extension in yeast.

Authors

Herbert, A.P., Lian, L., Morgan, A., Riesen, M., Bloxam, L., Kosmidou, E., Wareing, B.M., Phelan, J., Pennington, S.R.

Assembly

HSP12 SDS

Entity

1. HSP12 SDS (polymer, Thiol state: not present), 109 monomers, 11692.56 Da Detail

MSDAGRKGFG EKASEALKPD SQKSYAEQGK EYITDKADKV AGKVQPEDNK GVFQGVHDSA EKGKDNAEGQ GESLADQARD YMGAAKSKLN DAVEYVSGRV HGEEDPTKK

Formula weight

11692.56 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

solution NMR

Refine. method

NONE

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 95.0 %, Completeness (bb): 98.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	95.0 % (1136 of 1196)	95.6 % (603 of 631)	94.2 % (424 of 450)	94.8 % (109 of 115)
Backbone	98.0 % (635 of 648)	96.5 % (220 of 228)	98.7 % (310 of 314)	99.1 % (105 of 106)
Sidechain	92.5 % (596 of 644)	95.0 % (383 of 403)	90.1 % (209 of 232)	44.4 % (4 of 9)
Aromatic	86.7 % (52 of 60)	86.7 % (26 of 30)	86.7 % (26 of 30)
Methyl	100.0 % (74 of 74)	100.0 % (37 of 37)	100.0 % (37 of 37)

1. HSP12 SDS

MSDAGRKGFG EKASEALKPD SQKSYAEQGK EYITDKADKV AGKVQPEDNK GVFQGVHDSA EKGKDNAEGQ GESLADQARD YMGAAKSKLN DAVEYVSGRV HGEEDPTKK

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 318.000 K, pH 6.500

#	Name	Isotope labeling	Concentration
1	HSP12_SDS	[U-13C; U-15N]	0.45 mM
2	SDS	natural abundance	100.00 mM
3	NaCl	natural abundance	40.00 mM
4	Potassium Phosphate	natural abundance	20.00 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 318.000 K, pH 6.500

#	Name	Isotope labeling	Concentration
5	SDS	natural abundance	100.00 mM
6	NaCl	natural abundance	40.00 mM
7	Potassium Phosphate	natural abundance	20.00 mM
8	HSP12_SDS	[U-15N]	0.45 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	HDO	H	0.0 ppm	internal	indirect	1.0

Referencing offset: -0.38 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	HDO	H	0.0 ppm	internal	indirect	1.0

Referencing offset: -0.38 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	HDO	H	0.0 ppm	internal	indirect	1.0

Referencing offset: 0.04 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	HDO	H	0.0 ppm	internal	indirect	1.0

Referencing offset: -0.04 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 4AXP, Strand ID: A Detail

Release date

2012-06-13

Citation

NMR structure of Hsp12, a protein induced by and required for dietary restriction-induced lifespan extension in yeast
Herbert, A.P., Riesen, M., Bloxam, L., Kosmidou, E., Wareing, B.M., Johnson, J.R., Phelan, M.M., Pennington, S.R., Lian, L., Morgan, A.
PLoS One (2012), 7, e41975-e41975, PubMed 22848679 , DOI 10.1371/journal.pone.0041975 , Abstract

Related entities 1. HSP12 SDS, : 1 : 4 : 3 entities Detail

Interaction partners 1. HSP12 SDS, : 104 interactors Detail

More details for 104 interactors identified by 4 citations

Experiments performed 16 experiments Detail

1 2D 1H-15N HSQC/HMQC