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BMRB: 18546

2LUY

Solution structure of the tandem zinc finger domain of fission yeast Stc1

Authors

He, C., Shi, Y.YU., Bayne, E., Wu, H.JI.

Assembly

tandem zinc finger domain of Stc1

Entity

1. tandem zinc finger domain of Stc1, entity 1 (polymer, Thiol state: all other bound), 97 monomers, 11202.84 Da Detail

HMGKNDNDAL IMCMRCRKVK GIDSYSKTQW SKTFTFVRGR TVSVSDPKVI CRTCQPKQHD SIWCTACQQT KGINEFSKAQ RHVLDPRCQI CVHSQRN

2. tandem zinc finger domain of Stc1, entity ZN (non-polymer), 65.409 × 2 Da

Total weight

11333.658 Da

Max. entity weight

11202.84 Da

Entity Connection

metal coordination 8 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	metal coordination	sing	1:CYS51:SG	2:ZN1:ZN
2	metal coordination	sing	1:CYS88:SG	2:ZN1:ZN
3	metal coordination	sing	1:CYS64:SG	2:ZN1:ZN
4	metal coordination	sing	1:CYS13:SG	2:ZN1:ZN
5	metal coordination	sing	1:CYS16:SG	2:ZN1:ZN
6	metal coordination	sing	1:CYS67:SG	2:ZN1:ZN
7	metal coordination	sing	1:CYS91:SG	2:ZN1:ZN
8	metal coordination	sing	1:CYS54:SG	2:ZN1:ZN

Source organism

Schizosaccharomyces pombe

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 90.2 %, Completeness (bb): 96.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	90.2 % (1034 of 1146)	90.9 % (550 of 605)	87.8 % (380 of 433)	96.3 % (104 of 108)
Backbone	96.9 % (558 of 576)	95.9 % (187 of 195)	96.9 % (278 of 287)	98.9 % (93 of 94)
Sidechain	85.8 % (569 of 663)	88.5 % (363 of 410)	81.6 % (195 of 239)	78.6 % (11 of 14)
Aromatic	39.7 % (31 of 78)	71.8 % (28 of 39)	2.7 % (1 of 37)	100.0 % (2 of 2)
Methyl	97.5 % (78 of 80)	95.0 % (38 of 40)	100.0 % (40 of 40)

1. entity 1

HMGKNDNDAL IMCMRCRKVK GIDSYSKTQW SKTFTFVRGR TVSVSDPKVI CRTCQPKQHD SIWCTACQQT KGINEFSKAQ RHVLDPRCQI CVHSQRN

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	sodium chloride	natural abundance	200 mM
2	Bis-Tris	natural abundance	20 mM
3	entity_1	[U-100% 13C; U-100% 15N]	0.8 mM
4	ZINC ION	natural abundance	1.6 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.773 ppm	internal	indirect	0.2514495
¹H	water	protons	4.773 ppm	internal	direct	1.0
¹⁵N	water	protons	4.773 ppm	internal	indirect	0.1013291

Referencing offset: -0.07 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.773 ppm	internal	indirect	0.2514495
¹H	water	protons	4.773 ppm	internal	direct	1.0
¹⁵N	water	protons	4.773 ppm	internal	indirect	0.1013291

Referencing offset: -0.07 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.773 ppm	internal	indirect	0.2514495
¹H	water	protons	4.773 ppm	internal	direct	1.0
¹⁵N	water	protons	4.773 ppm	internal	indirect	0.1013291

Referencing offset: -0.01 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.773 ppm	internal	indirect	0.2514495
¹H	water	protons	4.773 ppm	internal	direct	1.0
¹⁵N	water	protons	4.773 ppm	internal	indirect	0.1013291

Referencing offset: 0.29 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2LUY, Strand ID: A Detail

Release date

2013-05-05

Citation

Structural analysis of Stc1 provides insights into the coupling of RNAi and chromatin modification
He, C., Pillai, S.S., Taglini, F., Li, F., Ruan, K., Zhang, J., Wu, J., Shi, Y., Bayne, E.H.
Proc. Natl. Acad. Sci. U. S. A. (2013), 110, E1879-E1888, PubMed 23613586 , DOI 10.1073/pnas.1212155110 , Abstract

Related entities 1. tandem zinc finger domain of Stc1, entity 1, : 1 : 2 : 1 entities Detail

Interaction partners 1. tandem zinc finger domain of Stc1, entity 1, : 8 interactors Detail

More details for 8 interactors identified by 1 citations

Experiments performed 13 experiments Detail

1 2D 1H-15N HSQC