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BMRB: 18548

2M5H

1H, 13C, and 15N resonance assignments of the monomeric human Fam96a

Authors

Xia, B., Ouyang, B.

Assembly

Fam96a

Entity

1. Fam96a (polymer, Thiol state: all free), 143 monomers, 16781.97 Da Detail

MRQPRIMEEK ALEVYDLIRT IRDPEKPNTL EELEVVSESC VEVQEINEEE YLVIIRFTPT VPHCSLATLI GLCLRVKLQR CLPFKHKLEI YISEGTHSTE EDINKQINDK ERVAAAMENP NLREIVEQCV LEPDQLEHHH HHH

Formula weight

16781.97 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics, simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.2 %, Completeness: 91.4 %, Completeness (bb): 93.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	91.4 % (1565 of 1713)	93.2 % (838 of 899)	89.1 % (594 of 667)	90.5 % (133 of 147)
Backbone	93.7 % (789 of 842)	94.6 % (265 of 280)	94.4 % (403 of 427)	89.6 % (121 of 135)
Sidechain	89.4 % (905 of 1012)	91.9 % (569 of 619)	85.3 % (325 of 381)	91.7 % (11 of 12)
Aromatic	22.5 % (18 of 80)	45.0 % (18 of 40)	0.0 % (0 of 40)
Methyl	95.5 % (168 of 176)	95.5 % (84 of 88)	95.5 % (84 of 88)

1. Fam96a

MRQPRIMEEK ALEVYDLIRT IRDPEKPNTL EELEVVSESC VEVQEINEEE YLVIIRFTPT VPHCSLATLI GLCLRVKLQR CLPFKHKLEI YISEGTHSTE EDINKQINDK ERVAAAMENP NLREIVEQCV LEPDQLEHHH HHH

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	Fam96a	[U-100% 13C; U-100% 15N]	0.5 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	50 mM
4	EDTA	natural abundance	1 mM
5	DTT	natural abundance	50 mM
6	ARG	natural abundance	50 mM
7	GLU	natural abundance	50 mM
8	D2O	natural abundance	5 %
9	H2O	natural abundance	95 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.28 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.28 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.01 ppm, Outliers: 3 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.82 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2M5H, Strand ID: A Detail

Release date

2013-09-11

Citation

Solution structure of monomeric human FAM96A
Ouyang, B., Wang, L., Wan, S., Luo, Y., Wang, L., Lin, J., Xia, B.
J. Biomol. NMR (2013), 56, 387-392, PubMed 23793605 , DOI 10.1007/s10858-013-9746-6 , Abstract

Related entities 1. Fam96a, : 1 : 4 : 37 entities Detail

Interaction partners 1. Fam96a, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 7 experiments Detail

1 2D 1H-15N HSQC