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BMRB: 18550

2LV1

Solution-state NMR of prion protein mutant V210I at pH 7

Authors

Biljan, I., Ilc, G., Giachin, G., Legname, G., Plavec, J.

Assembly

V210I

Entity

1. V210I (polymer, Thiol state: all disulfide bound), 147 monomers, 16633.37 Da Detail

GAMDPGQGGG THSQWNKPSK PKTNMKHMAG AAAAGAVVGG LGGYMLGSAM SRPIIHFGSD YEDRYYRENM HRYPNQVYYR PMDEYSNQNN FVHDCVNITI KQHTVTTTTK GENFTETDVK MMERVIEQMC ITQYERESQA YYQRGSS

Formula weight

16633.37 Da

Entity Connection

disulfide 1 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS95:SG	1:CYS130:SG

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

molecular dynamics, simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.3 %, Completeness: 84.3 %, Completeness (bb): 77.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	84.3 % (1412 of 1675)	90.5 % (792 of 875)	74.5 % (477 of 640)	89.4 % (143 of 160)
Backbone	77.5 % (674 of 870)	89.4 % (271 of 303)	65.0 % (277 of 426)	89.4 % (126 of 141)
Sidechain	92.2 % (864 of 937)	91.1 % (521 of 572)	94.2 % (326 of 346)	89.5 % (17 of 19)
Aromatic	85.7 % (132 of 154)	85.7 % (66 of 77)	85.5 % (65 of 76)	100.0 % (1 of 1)
Methyl	98.1 % (102 of 104)	96.2 % (50 of 52)	100.0 % (52 of 52)

1. V210I

GAMDPGQGGG THSQWNKPSK PKTNMKHMAG AAAAGAVVGG LGGYMLGSAM SRPIIHFGSD YEDRYYRENM HRYPNQVYYR PMDEYSNQNN FVHDCVNITI KQHTVTTTTK GENFTETDVK MMERVIEQMC ITQYERESQA YYQRGSS

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 7.2, Details 20 mM Tris buffer 20 mM KCl pH 7.2

#	Name	Isotope labeling	Concentration
1	V210I	[U-100% 13C; U-100% 15N]	0.6 mM
2	H2O	natural abundance	90 %
3	D2O	[U-2H]	10 %
4	Tris buffer	natural abundance	20 mM
5	KCl	natural abundance	20 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.07 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.07 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.07 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2LV1, Strand ID: A Detail

Release date

2012-09-16

Citation

Structural rearrangements at physiological pH: nuclear magnetic resonance insights from the V210I human prion protein mutant
Biljan, I., Ilc, G., Giachin, G., Plavec, J., Legname, G.
Biochemistry (2012), 51, 7465-7474, PubMed 22947063 , DOI 10.1021/bi3009856 , Abstract

Related entities 1. V210I, : 1 : 1 : 99 entities Detail

Interaction partners 1. V210I, : 73 interactors Detail

More details for 73 interactors identified by 15 citations

Experiments performed 7 experiments Detail

1 2D 1H-15N HSQC