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BMRB: 18553

2LV4

ZirS C-terminal Domain

Authors

Prehna, G., Li, Y., Stoynov, N., Okon, M., Vukovic, M., McIntosh, L.P., Foster, L.J., Finlay, B., Strynadka, N.C.

Assembly

ZirS C-terminal Domain

Entity

1. ZirS C-terminal Domain (polymer, Thiol state: not present), 146 monomers, 16086.95 Da Detail

GPLGSGSKIK LEIYNETDMA SASGYTPVPS VSEFQYIETE TISNTPSPDL TVMSIDKSVL SPGESATITT IVKDIDGNPV NEVHINKTVA RENLKGLWDY GPLKKENVPG KYTQVITYRG HSNERIDISF KYAMSFTKEI SIRGRL

Formula weight

16086.95 Da

Source organism

Salmonella enterica subsp. enterica serovar Typhimurium

Exptl. method

solution NMR

Refine. method

simulated annealing, distance geometry

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.6 %, Completeness: 86.0 %, Completeness (bb): 91.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	86.0 % (1442 of 1676)	87.1 % (758 of 870)	84.8 % (558 of 658)	85.1 % (126 of 148)
Backbone	91.4 % (784 of 858)	88.4 % (260 of 294)	94.6 % (404 of 427)	87.6 % (120 of 137)
Sidechain	82.9 % (790 of 953)	86.5 % (498 of 576)	78.1 % (286 of 366)	54.5 % (6 of 11)
Aromatic	83.0 % (88 of 106)	90.6 % (48 of 53)	76.9 % (40 of 52)	0.0 % (0 of 1)
Methyl	88.4 % (145 of 164)	98.8 % (81 of 82)	78.0 % (64 of 82)

1. ZirS

GPLGSGSKIK LEIYNETDMA SASGYTPVPS VSEFQYIETE TISNTPSPDL TVMSIDKSVL SPGESATITT IVKDIDGNPV NEVHINKTVA RENLKGLWDY GPLKKENVPG KYTQVITYRG HSNERIDISF KYAMSFTKEI SIRGRL

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K, pH 7.1

#	Name	Isotope labeling	Concentration
1	ZirS	[U-100% 13C; U-100% 15N]	0.5 mM
2	TRIS	natural abundance	50 mM
3	sodium chloride	natural abundance	50 mM
4	TCEP	natural abundance	0.1 mM
5	PMSF	natural abundance	0.1 mM
6	H2O	natural abundance	95 %
7	D2O	natural abundance	5 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	liquid anhydrous ammonia	methyl protons	null	indirect	0.1013291

Referencing offset: -0.13 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	liquid anhydrous ammonia	methyl protons	null	indirect	0.1013291

Referencing offset: -0.13 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	liquid anhydrous ammonia	methyl protons	null	indirect	0.1013291

Referencing offset: -0.07 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	liquid anhydrous ammonia	methyl protons	null	indirect	0.1013291

Referencing offset: 0.58 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 2LV4, Strand ID: A Detail

Release date

2012-08-20

Citation

The zinc regulated antivirulence pathway of Salmonella is a multiprotein immunoglobulin adhesion system
Prehna, G., Li, Y., Stoynov, N., Okon, M., Vuckovic, M., McIntosh, L.P., Foster, L.J., Finlay, B., Strynadka, N.C.J.
J. Biol. Chem. (2012), 287, 32324-32337, PubMed 22810234 , DOI 10.1074/jbc.M112.357210 , Abstract

Related entities 1. ZirS C-terminal Domain, : 1 : 2 entities Detail

Experiments performed 13 experiments Detail

1 3D HNCACB