BMRBj - BMREntryMaster

Found 1 Document (0.748 seconds)

BMRB: 18558

2LV8

Solution NMR Structure de novo designed rossmann 2x2 fold protein, Northeast Structural Genomics Consortium (NESG) Target OR16

Authors: Liu, G., Koga, R., Koga, N., Xiao, R., Pederson, K., Hamilton, K., Ciccosanti, C., Acton, T.B., Everett, J.K., Baker, D., Montelione, G.T.
Assembly: OR16
Entity: 1. OR16 (polymer, Thiol state: not present), 110 monomers, 13050.78 Da Detail
MLLYVLIISN DKKLIEEARK MAEKANLELR TVKTEDELKK YLEEFRKESQ NIKVLILVSN DEELDKAKEL AQKMEIDVRT RKVTSPDEAK RWIKEFSEEG GSLEHHHHHH
Formula weight: 13050.78 Da
Exptl. method: solution NMR
Refine. method: distance geometry, simulated annealing, torsion angle dynamics, molecular dynamics
Data set: RDCs, spectral_peak_list
Protein Blocks Logo: Calculated from 20 models in PDB: 2LV8, Strand ID: A Detail
RDC: 170 RDC values in 2 lists
Field strength (¹H) 599.520 MHz, Pressure 1 atm, Temperature 298 K, pH 6.5 Detail
Pressure 1 atm, Temperature 298 K, pH 6.5
Experiment name 2D 1H-15N HSQC
List #1 RDC_list_1, RDC code DHN, Field strength (¹H) 599.520 MHz, Details alignment medias: peg for Ori 1 and phage for Ori 2. # Orientation Magnitude Rhombicity ORI residue number 1 -7.005 0.392 118 2 10.199 0.418 125
List #2 RDC_list_2, RDC code DHN, Field strength (¹H) 599.520 MHz, Details alignment medias: peg for Ori 1 and phage for Ori 2. # Orientation Magnitude Rhombicity ORI residue number # 2 -12.87 0.278 125
Release date: 2012-07-30
Citation: Principles for designing ideal protein structures
Koga, N., Tatsumi-Koga, R., Liu, G., Xiao, R., Acton, T.B., Montelione, G.T., Baker, D.
Nature (2012), 491, 222-227, PubMed 23135467 , DOI 10.1038/nature11600 , Abstract

Related entities 1. OR16, : 1 : 1 : 80 entities Detail

Experiments performed 11 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 800 MHz Rutgers

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.073 mM OR16.004, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	OR16.004	[U-100% 13C; U-100% 15N]	1.073 mM
2	Proteinase Inhibitors	natural abundance	1 na
3	NaN3	natural abundance	0.02 %
4	DTT	natural abundance	10 mM
5	CaCL2	natural abundance	5 mM
6	NaCL	natural abundance	200 mM
7	MES pH 6.5	natural abundance	20 mM
8	D2O	natural abundance	10 %
9	DSS	natural abundance	50 uM

2 2D 1H-13C HSQC

Instrument

Bruker Avance - 800 MHz Rutgers

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.073 mM OR16.004, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	OR16.004	[U-100% 13C; U-100% 15N]	1.073 mM
2	Proteinase Inhibitors	natural abundance	1 na
3	NaN3	natural abundance	0.02 %
4	DTT	natural abundance	10 mM
5	CaCL2	natural abundance	5 mM
6	NaCL	natural abundance	200 mM
7	MES pH 6.5	natural abundance	20 mM
8	D2O	natural abundance	10 %
9	DSS	natural abundance	50 uM

3 3D HNCO

Instrument

Varian INOVA - 600 MHz Rutgers

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.073 mM OR16.004, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	OR16.004	[U-100% 13C; U-100% 15N]	1.073 mM
2	Proteinase Inhibitors	natural abundance	1 na
3	NaN3	natural abundance	0.02 %
4	DTT	natural abundance	10 mM
5	CaCL2	natural abundance	5 mM
6	NaCL	natural abundance	200 mM
7	MES pH 6.5	natural abundance	20 mM
8	D2O	natural abundance	10 %
9	DSS	natural abundance	50 uM

4 3D CBCA(CO)NH

Instrument

Varian INOVA - 600 MHz Rutgers

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.073 mM OR16.004, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	OR16.004	[U-100% 13C; U-100% 15N]	1.073 mM
2	Proteinase Inhibitors	natural abundance	1 na
3	NaN3	natural abundance	0.02 %
4	DTT	natural abundance	10 mM
5	CaCL2	natural abundance	5 mM
6	NaCL	natural abundance	200 mM
7	MES pH 6.5	natural abundance	20 mM
8	D2O	natural abundance	10 %
9	DSS	natural abundance	50 uM

5 3D HNCACB

Instrument

Varian INOVA - 600 MHz Rutgers

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.073 mM OR16.004, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	OR16.004	[U-100% 13C; U-100% 15N]	1.073 mM
2	Proteinase Inhibitors	natural abundance	1 na
3	NaN3	natural abundance	0.02 %
4	DTT	natural abundance	10 mM
5	CaCL2	natural abundance	5 mM
6	NaCL	natural abundance	200 mM
7	MES pH 6.5	natural abundance	20 mM
8	D2O	natural abundance	10 %
9	DSS	natural abundance	50 uM

6 3D 1H-13C arom NOESY

Instrument

Bruker Avance - 800 MHz Rutgers

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.073 mM OR16.004, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	OR16.004	[U-100% 13C; U-100% 15N]	1.073 mM
2	Proteinase Inhibitors	natural abundance	1 na
3	NaN3	natural abundance	0.02 %
4	DTT	natural abundance	10 mM
5	CaCL2	natural abundance	5 mM
6	NaCL	natural abundance	200 mM
7	MES pH 6.5	natural abundance	20 mM
8	D2O	natural abundance	10 %
9	DSS	natural abundance	50 uM

7 3D simutaneous 13C-aromatic,13C-aliphatic,15N edited 1H-1H NOESY

Instrument

Bruker Avance - 800 MHz Rutgers

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.073 mM OR16.004, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	OR16.004	[U-100% 13C; U-100% 15N]	1.073 mM
2	Proteinase Inhibitors	natural abundance	1 na
3	NaN3	natural abundance	0.02 %
4	DTT	natural abundance	10 mM
5	CaCL2	natural abundance	5 mM
6	NaCL	natural abundance	200 mM
7	MES pH 6.5	natural abundance	20 mM
8	D2O	natural abundance	10 %
9	DSS	natural abundance	50 uM

8 3D C(CO)NH

Instrument

Varian INOVA - 600 MHz Rutgers

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.073 mM OR16.004, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	OR16.004	[U-100% 13C; U-100% 15N]	1.073 mM
2	Proteinase Inhibitors	natural abundance	1 na
3	NaN3	natural abundance	0.02 %
4	DTT	natural abundance	10 mM
5	CaCL2	natural abundance	5 mM
6	NaCL	natural abundance	200 mM
7	MES pH 6.5	natural abundance	20 mM
8	D2O	natural abundance	10 %
9	DSS	natural abundance	50 uM

9 3D HCCH-TOCSY

Instrument

Varian INOVA - 600 MHz Rutgers

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1.073 mM OR16.004, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	OR16.004	[U-100% 13C; U-100% 15N]	1.073 mM
2	Proteinase Inhibitors	natural abundance	1 na
3	NaN3	natural abundance	0.02 %
4	DTT	natural abundance	10 mM
5	CaCL2	natural abundance	5 mM
6	NaCL	natural abundance	200 mM
7	MES pH 6.5	natural abundance	20 mM
8	D2O	natural abundance	10 %
9	DSS	natural abundance	50 uM

10 2D 1H-13C HSQC

Instrument

Varian INOVA - 600 MHz Rutgers

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.867 mM OR16.006, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
10	OR16.006	[U-100% 13C; U-100% 15N]	0.867 mM
11	Proteinase Inhibitors	natural abundance	1 na
12	NaN3	natural abundance	0.02 %
13	DTT	natural abundance	10 mM
14	CaCL2	natural abundance	5 mM
15	NaCL	natural abundance	200 mM
16	MES pH 6.5	natural abundance	20 mM
17	D2O	natural abundance	10 %
18	DSS	natural abundance	50 uM

11 2D 1H-15N HSQC

Instrument

Varian INOVA - 600 MHz UGA

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 0.859 mM OR16.013, 90% H2O/10% D2O alignment media peg or phage

#	Name	Isotope labeling	Concentration
19	OR16.013	[U-100% 13C; U-100% 15N]	0.859 mM
20	Proteinase Inhibitors	natural abundance	1 na
21	NaN3	natural abundance	0.02 %
22	DTT	natural abundance	10 mM
23	CaCL2	natural abundance	5 mM
24	NaCL	natural abundance	200 mM
25	MES pH 6.5	natural abundance	20 mM
26	D2O	natural abundance	10 %
27	DSS	natural abundance	50 uM

Keywords NORTHEAST STRUCTURAL GENOMICS CONSORTIUM (NESG), PSI-Biology, Protein NMR, Protein Structure Initiative, Structural Genomics, Target OR16

Search

Query history

Resources

Search for primary databases

Search for subsidiary databases

Display

Hits per page

Statistics

Query Changes will take effect after "OK" button is selected.

Maximum hits

Default conjunction operator

Omni-box

Search filter

Sequence search (BLAST)

E-value

Query sequence coverage

Miscellaneous

The number of previewed publications

Completeness of assigned chemical shift

Sequence chart about top aligned regions

Sequence chart about interacting regions

Hit context with highlight

Link destinations for BMRB, PDB and EMDB

Found 1 Document (0.748 seconds)

BMRB: 18558

Related entities 1. OR16, : 1 : 1 : 80 entities Detail

Experiments performed 11 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample