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BMRB: 18560

2LVA

NMR solution structure of the N-terminal domain of human USP28. Northeast structural genomics consortium target HT8470A

Authors

Lemak, A., Yee, A., Houliston, S., Garcia, M., Dhe-Paganon, S., Arrowsmith, C.

Assembly

hs356 22 132

Entity

1. hs356 22 132 (polymer, Thiol state: not present), 129 monomers, 14225.56 Da Detail

MHHHHHHSSG RENLYFQGQM LLNQLREITG IQDPSFLHEA LKASNGDITQ AVSLLTDERV KEPSQDTVAT EPSEVEGSAA NKEVLAKVID LTHDNKDDLQ AAIALSLLES PKIQADGRDL NRMHEATSA

Formula weight

14225.56 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

restrained molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 86.0 %, Completeness: 79.2 %, Completeness (bb): 83.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	79.2 % (1141 of 1441)	79.9 % (596 of 746)	77.3 % (430 of 556)	82.7 % (115 of 139)
Backbone	83.7 % (641 of 766)	84.2 % (219 of 260)	83.5 % (318 of 381)	83.2 % (104 of 125)
Sidechain	75.9 % (606 of 798)	77.6 % (377 of 486)	73.2 % (218 of 298)	78.6 % (11 of 14)
Aromatic	12.5 % (8 of 64)	18.8 % (6 of 32)	6.3 % (2 of 32)
Methyl	81.1 % (120 of 148)	82.4 % (61 of 74)	79.7 % (59 of 74)

1. hs356 22 132

MHHHHHHSSG RENLYFQGQM LLNQLREITG IQDPSFLHEA LKASNGDITQ AVSLLTDERV KEPSQDTVAT EPSEVEGSAA NKEVLAKVID LTHDNKDDLQ AAIALSLLES PKIQADGRDL NRMHEATSA

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	hs356_22_132	[U-13C; U-15N]	0.5 mM
2	mops	natural abundance	10 mM
3	sodium chloride	natural abundance	450 mM
4	ZnSO4	natural abundance	10 uM
5	DTT	natural abundance	1 mM
6	NaN3	natural abundance	0.01 %
7	benzamidine	natural abundance	1 mM
8	H2O	natural abundance	90 %
9	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.15 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.15 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.1 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.14 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2LVA, Strand ID: A Detail

Release date

2012-07-30

Citation

The N-terminal ubiquitin-binding region of ubiquitin-specific protease 28 modulates its deubiquitination function: NMR structural and mechanistic insights
Lemak, A., Yee, A., Houliston, S., Garcia, M., Dhe-Paganon, S., Arrowsmith, C.
Biochem. J. (2015), 471, 155-165, PubMed 26268556 , DOI 10.1042/BJ20150088 , Abstract

Related entities 1. hs356 22 132, : 1 : 2 : 5 entities Detail

Interaction partners 1. hs356 22 132, : 11 interactors Detail

More details for 11 interactors identified by 9 citations

Experiments performed 9 experiments Detail

1 3D HNCO