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BMRB: 18570

2LVH

Solution structure of the zinc finger AFV1p06 protein from the hyperthermophilic archaeal virus AFV1

Authors

Guilliere, F., Sezonov, G., Prangishvili, D., Delepierre, M., Guijarro, J.

Assembly

zinc finger AFV1p06 protein

Entity

1. AFV1p06 (polymer), 59 monomers, 7000.149 Da Detail

MIEVSSMERV YQCLRCGLTF RTKKQLIRHL VNTEKVNPLS IDYYYQSFSV SLKDVNKII

2. ZINC ION (non-polymer), 65.409 Da

Total weight

7065.558 Da

Max. entity weight

7000.149 Da

Source organism

Acidianus filamentous virus 1

Exptl. method

solution NMR

Refine. method

simulated annealing, torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 92.7 %, Completeness (bb): 94.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	92.7 % (682 of 736)	90.9 % (350 of 385)	94.8 % (272 of 287)	93.8 % (60 of 64)
Backbone	94.3 % (332 of 352)	93.2 % (110 of 118)	94.9 % (167 of 176)	94.8 % (55 of 58)
Sidechain	91.9 % (406 of 442)	89.9 % (240 of 267)	95.3 % (161 of 169)	83.3 % (5 of 6)
Aromatic	92.9 % (52 of 56)	92.9 % (26 of 28)	92.9 % (26 of 28)
Methyl	97.3 % (72 of 74)	94.6 % (35 of 37)	100.0 % (37 of 37)

1. AFV1p06

MIEVSSMERV YQCLRCGLTF RTKKQLIRHL VNTEKVNPLS IDYYYQSFSV SLKDVNKII

Show sample condition

Sample

Solvent system 88% H2O/12% D2O, Pressure 1 atm, Temperature 298.15 (±0.1) K, pH 7.4, Details 50 mM Na-HEPES 150 mM NaCl 50uM ZnCl2 3 mM DTT 12% D2O pH 7.4

#	Name	Isotope labeling	Concentration
1	AFV1p06	[U-98% 13C; U-98% 15N]	0.8-1 mM
2	Na-HEPES	natural abundance	50 mM
3	NaCl	natural abundance	150 mM
4	ZnCl2	natural abundance	50 uM
5	DTT	natural abundance	3 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.26 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.26 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.02 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.37 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 2LVH, Strand ID: A Detail

Release date

2013-01-27

Citation

Solution structure of an archaeal DNA binding protein with an eukaryotic zinc finger fold
Guilliere, F., Danioux, C., Jaubert, C., Desnoues, N., Delepierre, M., Prangishvili, D., Sezonov, G., Guijarro, J.
PLoS One (2013), 8, e52908-e52908, PubMed 23326363 , DOI 10.1371/journal.pone.0052908 , Abstract

Related entities 1. AFV1p06, : 1 : 1 : 127 entities Detail

Experiments performed 16 experiments Detail

1 2D 1H-15N HSQC