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Found 1 Document (3.29 seconds)

BMRB: 18579

2LVM

Methylated Histone Complex

Authors

Cui, G., Botuyan, M., Mer, G.

Assembly

Methylated Histone Complex

Entity

1. Methylated Histone Complex, entity 1 (polymer, Thiol state: all free), 123 monomers, 13925.62 Da Detail

GHMNSFVGLR VVAKWSSNGY FYSGKITRDV GAGKYKLLFD DGYECDVLGK DILLCDPIPL DTEVTALSED EYFSAGVVKG HRKESGELYY SIEKEGQRKW YKRMAVILSL EQGNRLREQY GLG

2. Methylated Histone Complex, entity 2 (polymer, Thiol state: not present), 14 monomers, 1719.004 Da Detail

GAKRHRXVLR DNIQ

Total weight

15644.624 Da

Max. entity weight

13925.62 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.3 %, Completeness: 96.3 %, Completeness (bb): 94.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	96.3 % (1545 of 1604)	97.5 % (817 of 838)	95.7 % (595 of 622)	92.4 % (133 of 144)
Backbone	94.3 % (766 of 812)	95.1 % (272 of 286)	94.6 % (371 of 392)	91.8 % (123 of 134)
Sidechain	97.6 % (890 of 912)	97.8 % (540 of 552)	97.4 % (341 of 350)	90.0 % (9 of 10)
Aromatic	92.6 % (137 of 148)	95.9 % (71 of 74)	88.9 % (64 of 72)	100.0 % (2 of 2)
Methyl	100.0 % (138 of 138)	100.0 % (69 of 69)	100.0 % (69 of 69)

1. entity 1

GHMNSFVGLR VVAKWSSNGY FYSGKITRDV GAGKYKLLFD DGYECDVLGK DILLCDPIPL DTEVTALSED EYFSAGVVKG HRKESGELYY SIEKEGQRKW YKRMAVILSL EQGNRLREQY GLG

2. entity 2

GAKRHRXVLR DNIQ

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	entity_1	[U-100% 13C; U-100% 15N]	1.7 mM
2	entity_2	natural abundance	8.5 mM
3	sodium phosphate	natural abundance	25 mM
4	DSS	natural abundance	0.001 %
5	NaN3	natural abundance	0.001 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
6	entity_1	[U-100% 13C; U-100% 15N]	1.7 mM
7	entity_2	natural abundance	8.5 mM
8	sodium phosphate	natural abundance	25 mM
9	DSS	natural abundance	0.001 %
10	NaN3	natural abundance	0.001 %

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
11	entity_1	natural abundance	5 mM
12	entity_2	[U-100% 13C; U-100% 15N]	4 mM
13	sodium phosphate	natural abundance	25 mM
14	DSS	natural abundance	0.001 %
15	NaN3	natural abundance	0.001 %

Sample #4

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
16	entity_1	natural abundance	5 mM
17	entity_2	[U-100% 13C; U-100% 15N]	4 mM
18	sodium phosphate	natural abundance	25 mM
19	DSS	natural abundance	0.001 %
20	NaN3	natural abundance	0.001 %

Protein Blocks Logo

Calculated from 20 models in PDB: 2LVM, Strand ID: A, B Detail

Release date

2012-12-05

Citation

Methylated Histone Complex
Cui, G., Botuyan, M., Mer, G.
Not known

Related entities 1. Methylated Histone Complex, entity 1, : 5 : 2 entities Detail

Related entities 2. Methylated Histone Complex, entity 2, : 1 : 7 : 5 : 3 entities Detail

Interaction partners 1. Methylated Histone Complex, entity 1, : 19 interactors Detail

More details for 19 interactors identified by 10 citations

Experiments performed 24 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	entity_1	[U-100% 13C; U-100% 15N]	1.7 mM
2	entity_2	natural abundance	8.5 mM
3	sodium phosphate	natural abundance	25 mM
4	DSS	natural abundance	0.001 %
5	NaN3	natural abundance	0.001 %

2 2D 1H-13C HSQC

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	entity_1	[U-100% 13C; U-100% 15N]	1.7 mM
2	entity_2	natural abundance	8.5 mM
3	sodium phosphate	natural abundance	25 mM
4	DSS	natural abundance	0.001 %
5	NaN3	natural abundance	0.001 %

3 2D 1H-13C HSQC aliphatic

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	entity_1	[U-100% 13C; U-100% 15N]	1.7 mM
2	entity_2	natural abundance	8.5 mM
3	sodium phosphate	natural abundance	25 mM
4	DSS	natural abundance	0.001 %
5	NaN3	natural abundance	0.001 %

4 2D 1H-13C HSQC aromatic

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	entity_1	[U-100% 13C; U-100% 15N]	1.7 mM
2	entity_2	natural abundance	8.5 mM
3	sodium phosphate	natural abundance	25 mM
4	DSS	natural abundance	0.001 %
5	NaN3	natural abundance	0.001 %

5 3D HNCA

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	entity_1	[U-100% 13C; U-100% 15N]	1.7 mM
2	entity_2	natural abundance	8.5 mM
3	sodium phosphate	natural abundance	25 mM
4	DSS	natural abundance	0.001 %
5	NaN3	natural abundance	0.001 %

6 3D HNCACB

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	entity_1	[U-100% 13C; U-100% 15N]	1.7 mM
2	entity_2	natural abundance	8.5 mM
3	sodium phosphate	natural abundance	25 mM
4	DSS	natural abundance	0.001 %
5	NaN3	natural abundance	0.001 %

7 3D CBCA(CO)NH

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	entity_1	[U-100% 13C; U-100% 15N]	1.7 mM
2	entity_2	natural abundance	8.5 mM
3	sodium phosphate	natural abundance	25 mM
4	DSS	natural abundance	0.001 %
5	NaN3	natural abundance	0.001 %

8 3D HNCO

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	entity_1	[U-100% 13C; U-100% 15N]	1.7 mM
2	entity_2	natural abundance	8.5 mM
3	sodium phosphate	natural abundance	25 mM
4	DSS	natural abundance	0.001 %
5	NaN3	natural abundance	0.001 %

9 3D HN(CA)CO

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	entity_1	[U-100% 13C; U-100% 15N]	1.7 mM
2	entity_2	natural abundance	8.5 mM
3	sodium phosphate	natural abundance	25 mM
4	DSS	natural abundance	0.001 %
5	NaN3	natural abundance	0.001 %

10 3D C(CO)NH

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	entity_1	[U-100% 13C; U-100% 15N]	1.7 mM
2	entity_2	natural abundance	8.5 mM
3	sodium phosphate	natural abundance	25 mM
4	DSS	natural abundance	0.001 %
5	NaN3	natural abundance	0.001 %

11 3D (H)CCH-TOCSY

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	entity_1	[U-100% 13C; U-100% 15N]	1.7 mM
2	entity_2	natural abundance	8.5 mM
3	sodium phosphate	natural abundance	25 mM
4	DSS	natural abundance	0.001 %
5	NaN3	natural abundance	0.001 %

12 3D HBHA(CO)NH

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	entity_1	[U-100% 13C; U-100% 15N]	1.7 mM
2	entity_2	natural abundance	8.5 mM
3	sodium phosphate	natural abundance	25 mM
4	DSS	natural abundance	0.001 %
5	NaN3	natural abundance	0.001 %

13 3D 1H-15N TOCSY

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	entity_1	[U-100% 13C; U-100% 15N]	1.7 mM
2	entity_2	natural abundance	8.5 mM
3	sodium phosphate	natural abundance	25 mM
4	DSS	natural abundance	0.001 %
5	NaN3	natural abundance	0.001 %

14 3D 1H-15N NOESY

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	entity_1	[U-100% 13C; U-100% 15N]	1.7 mM
2	entity_2	natural abundance	8.5 mM
3	sodium phosphate	natural abundance	25 mM
4	DSS	natural abundance	0.001 %
5	NaN3	natural abundance	0.001 %

15 3D 1H-13C NOESY aliphatic

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	entity_1	[U-100% 13C; U-100% 15N]	1.7 mM
2	entity_2	natural abundance	8.5 mM
3	sodium phosphate	natural abundance	25 mM
4	DSS	natural abundance	0.001 %
5	NaN3	natural abundance	0.001 %

16 3D 1H-13C NOESY aromatic

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	entity_1	[U-100% 13C; U-100% 15N]	1.7 mM
2	entity_2	natural abundance	8.5 mM
3	sodium phosphate	natural abundance	25 mM
4	DSS	natural abundance	0.001 %
5	NaN3	natural abundance	0.001 %

17 3D 13C/15N-filtered, 13C-edited NOESY

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
6	entity_1	[U-100% 13C; U-100% 15N]	1.7 mM
7	entity_2	natural abundance	8.5 mM
8	sodium phosphate	natural abundance	25 mM
9	DSS	natural abundance	0.001 %
10	NaN3	natural abundance	0.001 %

18 2D 1H-15N HSQC

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
11	entity_1	natural abundance	5 mM
12	entity_2	[U-100% 13C; U-100% 15N]	4 mM
13	sodium phosphate	natural abundance	25 mM
14	DSS	natural abundance	0.001 %
15	NaN3	natural abundance	0.001 %

19 2D 1H-13C HSQC

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
11	entity_1	natural abundance	5 mM
12	entity_2	[U-100% 13C; U-100% 15N]	4 mM
13	sodium phosphate	natural abundance	25 mM
14	DSS	natural abundance	0.001 %
15	NaN3	natural abundance	0.001 %

20 3D (H)CCH-TOCSY

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
11	entity_1	natural abundance	5 mM
12	entity_2	[U-100% 13C; U-100% 15N]	4 mM
13	sodium phosphate	natural abundance	25 mM
14	DSS	natural abundance	0.001 %
15	NaN3	natural abundance	0.001 %

21 3D 13C/15N-filtered, 13C-edited NOESY

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
16	entity_1	natural abundance	5 mM
17	entity_2	[U-100% 13C; U-100% 15N]	4 mM
18	sodium phosphate	natural abundance	25 mM
19	DSS	natural abundance	0.001 %
20	NaN3	natural abundance	0.001 %

22 3D 1H-15N NOESY

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
11	entity_1	natural abundance	5 mM
12	entity_2	[U-100% 13C; U-100% 15N]	4 mM
13	sodium phosphate	natural abundance	25 mM
14	DSS	natural abundance	0.001 %
15	NaN3	natural abundance	0.001 %

23 3D 1H-13C NOESY

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
11	entity_1	natural abundance	5 mM
12	entity_2	[U-100% 13C; U-100% 15N]	4 mM
13	sodium phosphate	natural abundance	25 mM
14	DSS	natural abundance	0.001 %
15	NaN3	natural abundance	0.001 %

24 2D (HB)CB(CGCD)HD

Instrument

Bruker Avance - 700 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	entity_1	[U-100% 13C; U-100% 15N]	1.7 mM
2	entity_2	natural abundance	8.5 mM
3	sodium phosphate	natural abundance	25 mM
4	DSS	natural abundance	0.001 %
5	NaN3	natural abundance	0.001 %

nullKeywords 53BP1, dimethylation, Histone H4 (H4K20me2), NMR spectroscopy

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Found 1 Document (3.29 seconds)

BMRB: 18579

Sample #1

Sample #2

Sample #3

Sample #4

Related entities 1. Methylated Histone Complex, entity 1, : 5 : 2 entities Detail

Related entities 2. Methylated Histone Complex, entity 2, : 1 : 7 : 5 : 3 entities Detail

Interaction partners 1. Methylated Histone Complex, entity 1, : 19 interactors Detail

Experiments performed 24 experiments Detail

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