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BMRB: 18586

2LVR

Solution structures of Miz-1 zinc fingers 8 to 10

Authors

Bedard, M., Maltais, L., Beaulieu, M., Bernard, D., Lavigne, P.

Assembly

MS1

Entity

1. Miz8-10 (polymer, Thiol state: all free), 83 monomers, 9496.887 Da Detail

MKPYVCIHCQ RQFADPGALQ RHVRIHTGEK PCQCVMCGKA FTQASSLIAH VRQHTGEKPY VCERCGKRFV QSSQLANHIR HHD

2. MS1, entity ZN (non-polymer), 65.409 Da

Total weight

9562.296 Da

Max. entity weight

9496.887 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.4 %, Completeness: 83.8 %, Completeness (bb): 94.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	83.8 % (812 of 969)	81.4 % (415 of 510)	86.3 % (320 of 371)	87.5 % (77 of 88)
Backbone	94.1 % (461 of 490)	94.0 % (157 of 167)	94.3 % (230 of 244)	93.7 % (74 of 79)
Sidechain	76.5 % (426 of 557)	75.2 % (258 of 343)	80.5 % (165 of 205)	33.3 % (3 of 9)
Aromatic	52.6 % (41 of 78)	53.8 % (21 of 39)	51.3 % (20 of 39)
Methyl	94.3 % (66 of 70)	100.0 % (35 of 35)	88.6 % (31 of 35)

1. Miz8-10

MKPYVCIHCQ RQFADPGALQ RHVRIHTGEK PCQCVMCGKA FTQASSLIAH VRQHTGEKPY VCERCGKRFV QSSQLANHIR HHD

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Type	Concentration
1	Miz8-10	[U-13C; U-15N]		0.75 ~ 1.0 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
¹H	water	protons	4.66 ppm	internal	indirect	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

Referencing offset: -0.12 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
¹H	water	protons	4.66 ppm	internal	indirect	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

Referencing offset: -0.12 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
¹H	water	protons	4.66 ppm	internal	indirect	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

Referencing offset: 0.1 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
¹H	water	protons	4.66 ppm	internal	indirect	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

Referencing offset: -0.06 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2LVR, Strand ID: A Detail

Release date

2013-07-15

Citation

NMR structure note: solution structure of human Miz-1 zinc fingers 8 to 10
Bedard, M., Maltais, L., Beaulieu, M., Bilodeau, J., Bernard, D., Lavigne, P.
J. Biomol. NMR (2012), 54, 317-323, PubMed 22986688 , DOI 10.1007/s10858-012-9670-1 , Abstract

Related entities 1. Miz8-10, : 1 : 2 : 2 : 2094 entities Detail

Interaction partners 1. Miz8-10, : 21 interactors Detail

More details for 21 interactors identified by 12 citations

Experiments performed 12 experiments Detail

1 2D 1H-15N HSQC