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BMRB: 18591

2LVW

Solution NMR studies of the dimeric regulatory subunit IlvN of the E.coli Acetohydroxyacid synthase I (AHAS I)

Authors

Karanth, M.N., Sarma, S.P.

Assembly

ilvN

Entity

1. ilvN (polymer, Thiol state: all free), 98 monomers, 11249.76 × 2 Da Detail

GSMQNTTHDN VILELTVRNH PGVMTHVCGL FARRAFNVEG ILCLPIQDSD KSHIWLLVND DQRLEQMISQ IDKLEDVVKV QRNQSDPTMF NKIAVFFQ

2. VALINE (non-polymer), 1 monomers, 117.146 × 2 Da

Total weight

22733.812 Da

Max. entity weight

11249.76 Da

Source organism

Escherichia coli

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.0 %, Completeness: 86.5 %, Completeness (bb): 92.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	86.5 % (1009 of 1166)	86.3 % (523 of 606)	86.4 % (388 of 449)	88.3 % (98 of 111)
Backbone	92.6 % (539 of 582)	92.9 % (183 of 197)	91.7 % (266 of 290)	94.7 % (90 of 95)
Sidechain	82.7 % (561 of 678)	83.1 % (340 of 409)	84.2 % (213 of 253)	50.0 % (8 of 16)
Aromatic	69.2 % (54 of 78)	71.8 % (28 of 39)	65.8 % (25 of 38)	100.0 % (1 of 1)
Methyl	95.8 % (115 of 120)	95.0 % (57 of 60)	96.7 % (58 of 60)

1. ilvN

GSMQNTTHDN VILELTVRNH PGVMTHVCGL FARRAFNVEG ILCLPIQDSD KSHIWLLVND DQRLEQMISQ IDKLEDVVKV QRNQSDPTMF NKIAVFFQ

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	IlvN	[U-98% 15N]	0.4 mM
2	potassium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	20 mM
4	EDTA	natural abundance	1 mM
5	sodium azide	natural abundance	0.01 %
6	L-Valine	natural abundance	5 mM
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 303 K, pH 7.0

#	Name	Isotope labeling	Concentration
9	IlvN	[U-98% 13C; U-98% 15N]	0.4 mM
10	potassium phosphate	natural abundance	20 mM
11	sodium chloride	natural abundance	20 mM
12	EDTA	natural abundance	1 mM
13	sodium azide	natural abundance	0.01 %
14	L-Valine	natural abundance	5 mM
15	H2O	natural abundance	90 %
16	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.31 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.31 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.08 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.23 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2LVW, Strand ID: A, B Detail

Release date

2012-07-23

Citation

The coil-to-helix transition in IlvN regulates the allosteric control of Escherichia coli acetohydroxyacid synthase I
Karanth, M.N., Sarma, S.P.
Biochemistry (2013), 52, 70-83, PubMed 23205845 , DOI 10.1021/bi301415m , Abstract

Related entities 1. ilvN, : 1 : 4 : 2 : 10 entities Detail

Experiments performed 15 experiments Detail

1 2D 1H-15N HSQC