MRH domain of the Glucosidase II beta subunit from S. pombe
YRAIKGMETK REIGGYTYKV VFYENVFQDS ILLGNFASQE GNVLKYENGQ SCWNGPHRSA IVTVECGVEN EIVSVLEAQK CEYLIKMKSP AACS
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | disulfide | sing | 1:CYS52:SG | 1:CYS66:SG |
2 | disulfide | sing | 1:CYS81:SG | 1:CYS93:SG |
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 90.6 % (991 of 1094) | 92.6 % (526 of 568) | 86.8 % (367 of 423) | 95.1 % (98 of 103) |
Backbone | 93.2 % (522 of 560) | 94.8 % (184 of 194) | 91.6 % (251 of 274) | 94.6 % (87 of 92) |
Sidechain | 88.9 % (551 of 620) | 91.4 % (342 of 374) | 84.3 % (198 of 235) | 100.0 % (11 of 11) |
Aromatic | 74.5 % (70 of 94) | 91.5 % (43 of 47) | 56.5 % (26 of 46) | 100.0 % (1 of 1) |
Methyl | 92.9 % (91 of 98) | 95.9 % (47 of 49) | 89.8 % (44 of 49) |
1. MRH domain
YRAIKGMETK REIGGYTYKV VFYENVFQDS ILLGNFASQE GNVLKYENGQ SCWNGPHRSA IVTVECGVEN EIVSVLEAQK CEYLIKMKSP AACSSolvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.1, Details 1.0 mM MRH(357-450) U-15N/13C, 10 mM imidazole, 150 mM sodium chloride
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | imidazole | [U-100% 2H] | 10 mM | |
2 | sodium chloride | natural abundance | 150 mM | |
3 | H2O | natural abundance | 95 % | |
4 | D2O | [U-99% 2H] | 5 % |