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BMRB: 18593


18593
2LVY
Solution Structure of a RNA Duplex Containing a 2'-O-Pivaloyloxymethyl Modification
Authors
Baraguey, C.
Assembly
RNA Duplex Containing a 2'-O-Pivaloyloxymethyl Modification
Entity
1. modified strand (polymer, Thiol state: not present), 9 monomers, 2982.861 Da Detail

CGCXACGCT


2. non-modified complementary strand (polymer, Thiol state: not present), 9 monomers, 2948.766 Da Detail

GCGUAGCGT


Total weight
5931.627 Da
Max. entity weight
2982.861 Da
Exptl. method
solution NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 83.3 %, Completeness: 99.2 %, Completeness (bb): 100.0 % Detail
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Polymer type: polyribonucleotide

Total1H
All99.2 % (132 of 133)99.2 % (132 of 133)
Suger, PO4100.0 % (90 of 90)100.0 % (90 of 90)
Nucleobase97.7 % (42 of 43)97.7 % (42 of 43)
Aromatic96.8 % (30 of 31)96.8 % (30 of 31)

1. modified strand

CGCXACGCX

2. non-modified complementary strand

GCGUAGCGX

Show sample condition
Sample #1

Solvent system 100% D2O, Pressure 1 atm, Temperature 293 K, pH 6.7, Details 5'-r(CGCU*ACGC)dT-3' 5'-r(GCGUAGCG)dT-3' 1:1


#NameIsotope labelingTypeConcentration
1DNA/RNA (5'-R(CP*GP*CP*(UPV)*AP*CP*GP*C)-D(P*T)-3')natural abundance0.9 mM
2DNA/RNA (5'-R(GP*CP*GP*UP*AP*CP*GP*C)-D(P*T)-3')natural abundance0.9 mM
Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.7, Details 5'-r(CGCU*ACGC)dT-3' 5'-r(GCGUAGCG)dT-3' 1:1


#NameIsotope labelingTypeConcentration
3DNA/RNA (5'-R(CP*GP*CP*(UPV)*AP*CP*GP*C)-D(P*T)-3')natural abundance0.9 mM
4DNA/RNA (5'-R(GP*CP*GP*UP*AP*CP*GP*C)-D(P*T)-3')natural abundance0.9 mM

Release date
2015-07-23
Citation
The biolabile 2'-O-pivaloyloxymethyl modification in an RNA helix: an NMR solution structure
Baraguey, C., Lescrinier, E., Lavergne, T., Debart, F., Herdewijn, P., Vasseur, J.
Org. Biomol. Chem. (2013), 11, 2638-2647, PubMed 23455628 , DOI 10.1039/c3ob27005j ,
Experiments performed 4 experiments Detail
NMR combined restraints 4 contents Detail
Keywords RNA, modification