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BMRB: 18595

5UK6

Solid-state NMR 13C and 15N resonance assignments of a seven-transmembrane helical protein Anabaena Sensory Rhodopsin

Authors

Wang, S., Shi, L.S., Brown, L.S., Ladizhansky, V.

Assembly

ASR

Entity

1. ASR (polymer, Thiol state: all free), 235 monomers, 27219.59 Da Detail

MNLESLLHWI YVAGMTIGAL HFWSLSRNPR GVPQYEYLVA MFIPIWSGLA YMAMAIDQGK VEAAGQIAHY ARYIDWMVTT PLLLLSLSWT AMQFIKKDWT LIGFLMSTQI VVITSGLIAD LSERDWVRYL WYICGVCAFL IILWGIWNPL RAKTRTQSSE LANLYDKLVT YFTVLWIGYP IVWIIGPSGF GWINQTIDTF LFCLLPFFSK VGFSFLDLHG LRNLNDSRQH HHHHH

Formula weight

27219.59 Da

Source organism

Trichormus variabilis

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 89.8 %, Completeness: 76.1 %, Completeness (bb): 87.0 % Detail

Polymer type: polypeptide(L)

	Total	¹³C	¹⁵N
All	76.1 % (1078 of 1417)	75.0 % (871 of 1162)	81.2 % (207 of 255)
Backbone	87.0 % (797 of 916)	86.4 % (595 of 689)	89.0 % (202 of 227)
Sidechain	64.3 % (463 of 720)	66.2 % (458 of 692)	17.9 % (5 of 28)
Aromatic	28.0 % (58 of 207)	28.9 % (56 of 194)	15.4 % (2 of 13)
Methyl	87.5 % (140 of 160)	87.5 % (140 of 160)

1. ASR

Show sample condition

Sample #1

Solvent system DMPC/DMPA, Pressure 1 atm, Temperature 278 K, pH 9.0, Details U-13C, U-15N anabaena sensory rhodospin in DMPC/DMPA lipids at the ratio of 2:1 (w/w)

#	Name	Isotope labeling	Concentration
1	ASR	[U-100% 13C; U-100% 15N]	0.0 ~ 0.0 w/w
2	DMPC	[U-100% 13C; U-100% 15N]	0.0 ~ 0.0 w/w
3	DMPA	[U-100% 13C; U-100% 15N]	0.0 ~ 0.0 w/w

Sample #2

Solvent system DMPC/DMPA, Pressure 1 atm, Temperature 278 K, pH 9.0, Details 2-13C glycerol, U-15N labeled anabaena sensory rhodopsin in DMPC/DMPA lipids at the ratio of 2:1

#	Name	Isotope labeling	Concentration
4	ASR	alternately labeled by 2-13C glycerol and U- 15N	0.0 ~ 0.0 w/w
5	DMPC	[U-100% 13C; U-100% 15N]	0.0 ~ 0.0 w/w
6	DMPA	[U-100% 13C; U-100% 15N]	0.0 ~ 0.0 w/w

Sample #3

Solvent system DMPC/DMPA, Pressure 1 atm, Temperature 278 K, pH 9.0, Details 1,3-C13 glycerol labeled and U-15N labeled anabaena sensory rhodopsin in DMPC/DMPA lipids at the ratio of 2:1 (w/w)

#	Name	Isotope labeling	Concentration
7	ASR	1,3-C13-glycerol labeled and U-N15 labeled	0.0 ~ 0.0 w/w
8	DMPC	[U-100% 13C; U-100% 15N]	0.0 ~ 0.0 w/w
9	DMPA	[U-100% 13C; U-100% 15N]	0.0 ~ 0.0 w/w

Chem. Shift Complete2

Sequence coverage: 21.3 %, Completeness: 44.4 %, Completeness (bb): 52.0 % Detail

Polymer type: polypeptide(L)

	Total	¹³C	¹⁵N
All	44.4 % (1259 of 2834)	43.2 % (1004 of 2324)	50.0 % (255 of 510)
Backbone	52.0 % (952 of 1832)	51.2 % (706 of 1378)	54.2 % (246 of 454)
Sidechain	36.6 % (527 of 1440)	37.4 % (518 of 1384)	16.1 % (9 of 56)
Aromatic	15.7 % (65 of 414)	16.2 % (63 of 388)	7.7 % (2 of 26)
Methyl	46.3 % (148 of 320)	46.3 % (148 of 320)

1. ASR

Show sample condition

Sample #1

Solvent system DMPC/DMPA, Pressure 1 atm, Temperature 278 K, pH 9.0, Details U-13C, U-15N anabaena sensory rhodospin in DMPC/DMPA lipids at the ratio of 2:1 (w/w)

#	Name	Isotope labeling	Concentration
1	ASR	[U-100% 13C; U-100% 15N]	0.0 ~ 0.0 w/w
2	DMPC	[U-100% 13C; U-100% 15N]	0.0 ~ 0.0 w/w
3	DMPA	[U-100% 13C; U-100% 15N]	0.0 ~ 0.0 w/w

Sample #2

Solvent system DMPC/DMPA, Pressure 1 atm, Temperature 278 K, pH 9.0, Details 2-13C glycerol, U-15N labeled anabaena sensory rhodopsin in DMPC/DMPA lipids at the ratio of 2:1

#	Name	Isotope labeling	Concentration
4	ASR	alternately labeled by 2-13C glycerol and U- 15N	0.0 ~ 0.0 w/w
5	DMPC	[U-100% 13C; U-100% 15N]	0.0 ~ 0.0 w/w
6	DMPA	[U-100% 13C; U-100% 15N]	0.0 ~ 0.0 w/w

Sample #3

#	Name	Isotope labeling	Concentration
7	ASR	1,3-C13-glycerol labeled and U-N15 labeled	0.0 ~ 0.0 w/w
8	DMPC	[U-100% 13C; U-100% 15N]	0.0 ~ 0.0 w/w
9	DMPA	[U-100% 13C; U-100% 15N]	0.0 ~ 0.0 w/w

Protein Blocks Logo

Calculated from 10 models in PDB: 2M3G, Strand ID: A, B, C Detail

Release date

2012-07-15

Citation

Solid-state NMR ¹³C and ¹⁵N resonance assignments of a seven-transmembrane helical protein Anabaena Sensory Rhodopsin
Wang, S., Shi, L., Okitsu, T., Wada, A., Brown, L.S., Ladizhansky, V.
Biomol. NMR Assign. (2013), 7, 253-256, PubMed 22983928 , DOI 10.1007/s12104-012-9421-y , Abstract

Related entities 1. ASR, : 1 : 1 : 1 : 98 entities Detail

Experiments performed 16 experiments Detail

1 2D NCA