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BMRB: 18605

2LW4

Solution NMR Structure of Human Transcription Elongation Factor A protein 2, Central Domain, Northeast Structural Genomics Consortium (NESG) Target HR8682B

Authors

Eletsky, A., Wang, D., Kohan, E., Janjua, H., Xiao, R., Acton, T.B., Everett, J.K., Montelione, G.T., Szyperski, T.

Assembly

HR8682B

Entity

1. HR8682B (polymer, Thiol state: all free), 113 monomers, 12716.47 Da Detail

SHMPVPVTCD AVRNKCREML TAALQTDHDH VAIGADCERL SAQIEECIFR DVGNTDMKYK NRVRSRISNL KDAKNPDLRR NVLCGAITPQ QIAVMTSEEM ASDELKEIRK AMT

Formula weight

12716.47 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete

Sequence coverage: 95.6 %, Completeness: 91.0 %, Completeness (bb): 93.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	91.0 % (1162 of 1277)	90.4 % (605 of 669)	91.8 % (449 of 489)	90.8 % (108 of 119)
Backbone	93.1 % (624 of 670)	93.3 % (210 of 225)	93.8 % (315 of 336)	90.8 % (99 of 109)
Sidechain	89.7 % (643 of 717)	89.0 % (395 of 444)	90.9 % (239 of 263)	90.0 % (9 of 10)
Aromatic	60.0 % (18 of 30)	60.0 % (9 of 15)	60.0 % (9 of 15)
Methyl	99.2 % (123 of 124)	100.0 % (62 of 62)	98.4 % (61 of 62)

1. HR8682B

SHMPVPVTCD AVRNKCREML TAALQTDHDH VAIGADCERL SAQIEECIFR DVGNTDMKYK NRVRSRISNL KDAKNPDLRR NVLCGAITPQ QIAVMTSEEM ASDELKEIRK AMT

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5, Details 1.0 mM HR8682B.004, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
1	HR8682B.004	[U-100% 13C; U-100% 15N]	1.0 mM
2	NaCl	natural abundance	100 mM
3	DTT	natural abundance	5 mM
4	NaN3	natural abundance	0.02 %
5	TRIS	natural abundance	10 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5, Details 1.0 mM HR8682B.004, 90% H2O/10% D2O

#	Name	Isotope labeling	Concentration
6	HR8682B.004	[5% 13C; U-100% 15N]	1.0 mM
7	NaCl	natural abundance	100 mM
8	DTT	natural abundance	5 mM
9	NaN3	natural abundance	0.02 %
10	TRIS	natural abundance	10 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.19 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.19 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.06 ppm, Outliers: 4 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.38 ppm, Outliers: 4 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2LW4, Strand ID: A Detail

Release date

2012-08-26

Related entities 1. HR8682B, : 1 : 2 : 74 entities Detail

Interaction partners 1. HR8682B, : 43 interactors Detail

More details for 43 interactors identified by 7 citations

Experiments performed 15 experiments Detail

1 2D 1H-15N HSQC