BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	87.5 % (1096 of 1252)	82.7 % (550 of 665)	93.2 % (449 of 482)	92.4 % (97 of 105)
Backbone	96.8 % (575 of 594)	95.5 % (191 of 200)	97.3 % (289 of 297)	97.9 % (95 of 97)
Sidechain	81.7 % (617 of 755)	77.2 % (359 of 465)	90.8 % (256 of 282)	25.0 % (2 of 8)
Aromatic	66.1 % (37 of 56)	71.4 % (20 of 28)	57.7 % (15 of 26)	100.0 % (2 of 2)
Methyl	96.6 % (114 of 118)	94.9 % (56 of 59)	98.3 % (58 of 59)

1. second CARD of human RIG-I

GSHMKKIEKL EEYRLLLKRL QPEFKTRIIP TDIISDLSEC LINQECEEIL QICSTKGMMA GAEKLVECLL RSDKENWPKT LKLALEKERN KFSELWIVEK

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K, pH 7.5, Details 15N labeled sample

#	Name	Isotope labeling	Concentration
1	card2	[U-100% 15N]	300 uM
2	H2O	natural abundance	95 %
3	D2O	natural abundance	5 %
4	Bis-Tris	natural abundance	50 mM
5	sodium chloride	natural abundance	250 mM

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K, pH 7.5, Details 13C-15N labeled sample

#	Name	Isotope labeling	Concentration
6	card2	[U-100% 13C; U-100% 15N]	300 uM
7	H2O	natural abundance	95 %
8	D2O	natural abundance	5 %
9	Bis-Tris	natural abundance	50 mM
10	sodium chloride	natural abundance	250 mM

Solvent system 100% D2O, Pressure 1 atm, Temperature 273 K, pH 7.5, Details 13C-15N labeled sample

#	Name	Isotope labeling	Concentration
11	card2	[U-100% 13C; U-100% 15N]	300 uM
12	D2O	natural abundance	100 %
13	Bis-Tris	natural abundance	50 mM
14	sodium chloride	natural abundance	250 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.13 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.13 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.0 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.23 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2LWD, Strand ID: A Detail

Release date

2012-10-18

Citation

Structure and dynamics of the second CARD of human RIG-I provide mechanistic insights into regulation of RIG-I activation
Ferrage, F., Dutta, K., Nistal-Villan, E., Patel, J.R., Sanchez-Aparicio, M.T., De Ioannes, P., Buku, A., Aseguinolaza, G., Garcia-Sastre, A., Aggarwal, A.K.
Structure (2012), 20, 2048-2061, PubMed 23063562 , DOI 10.1016/j.str.2012.09.003 , Abstract

1 2D 1H-15N HSQC

Bruker Avance - 500 MHz CryoProbe

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K, pH 7.5, Details 15N labeled sample

#	Name	Isotope labeling	Concentration
1	card2	[U-100% 15N]	300 uM
2	H2O	natural abundance	95 %
3	D2O	natural abundance	5 %
4	Bis-Tris	natural abundance	50 mM
5	sodium chloride	natural abundance	250 mM

2 3D HNCO