BMRBj - BMREntryMaster

Found 1 Document (1.469 seconds)

BMRB: 18627

5T7C

1H, 13C, and 15N Chemical Shift Assignments for Calcium free, Myristoylated Visinin-like protein 3

Authors

Li, C., Ames, J.B.

Assembly

VILIP-3 monomer

Entity

1. VILIP-3 monomer (polymer, Thiol state: all free), 193 monomers, 22312.93 Da Detail

MGKQNSKLRP EVLQDLRENT EFTDHELQEW YKGFLKDCPT GHLTVDEFKK IYANFFPYGD ASKFAEHVFR TFDTNGDGTI DFREFIIALS VTSRGKLEQK LKWAFSMYDL DGNGYISRSE MLEIVQAIYK MVSSVMKMPE DESTPEKRTD KIFRQMDTNN DGKLSLEEFI RGAKSDPSIV RLLQCDPSSA SQF

Formula weight

22312.93 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

DGSA-distance geometry simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.8 %, Completeness: 76.6 %, Completeness (bb): 91.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	76.6 % (1775 of 2317)	80.5 % (977 of 1214)	68.8 % (619 of 900)	88.2 % (179 of 203)
Backbone	91.4 % (1046 of 1144)	93.3 % (364 of 390)	89.3 % (507 of 568)	94.1 % (175 of 186)
Sidechain	66.1 % (896 of 1355)	74.3 % (612 of 824)	54.5 % (280 of 514)	23.5 % (4 of 17)
Aromatic	43.8 % (98 of 224)	85.7 % (96 of 112)	0.0 % (0 of 110)	100.0 % (2 of 2)
Methyl	95.3 % (162 of 170)	95.3 % (81 of 85)	95.3 % (81 of 85)

1. VILIP-3 monomer

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 7.4

#	Name	Isotope labeling	Concentration
1	Myristoylated VILIP-3	[U-100% 15N]	0.5 ~ 1.0 mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 7.4

#	Name	Isotope labeling	Concentration
4	Myristoylated VILIP-3	[U-100% 13C; U-100% 15N]	0.5-1 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 310 K, pH 7.4

#	Name	Isotope labeling	Type	Concentration
7	Myristoylated VILIP-3	[U-100% 13C; U-100% 15N]		0.5-1 mM
8	D2O	natural abundance		100 %

Sample #4

Solvent system 100% D2O, Pressure 1 atm, Temperature 310 K, pH 7.4

#	Name	Isotope labeling	Type	Concentration
9	Myristoylated VILIP-3	natural abundance		0.5-1 mM
10	D2O	natural abundance		100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.3 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.3 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.05 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.29 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 5T7C, Strand ID: A Detail

Release date

2016-09-15

Citation

¹H, ¹³C, and ¹⁵N chemical shift assignments of neuronal calcium sensor protein, hippocalcin
Li, C., Ames, J.B.
Biomol. NMR Assign. (2014), 8, 63-66, PubMed 23250791 , DOI 10.1007/s12104-012-9453-3 , Abstract

Search

Query history

Resources

Search for primary databases

Search for subsidiary databases

Display

Hits per page

Statistics

Query Changes will take effect after "OK" button is selected.

Maximum hits

Default conjunction operator

Omni-box

Search filter

Sequence search (BLAST)

E-value

Query sequence coverage

Miscellaneous

The number of previewed publications

Completeness of assigned chemical shift

Sequence chart about top aligned regions

Sequence chart about interacting regions

Hit context with highlight

Link destinations for BMRB, PDB and EMDB

Found 1 Document (1.469 seconds)

BMRB: 18627

Sample #1

Sample #2

Sample #3

Sample #4

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. VILIP-3 monomer, : 1 : 2 : 1 : 305 entities Detail

Interaction partners 1. VILIP-3 monomer, : 17 interactors Detail

Experiments performed 1 experiments Detail

Instrument

Sample