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Found 1 Document (5.256 seconds)

BMRB: 18632

6I9F

Resonance assignment of As-p18, a structurally unusual fatty acid binding protein secreted by developing larvae of the parasitic nematode Ascaris suum

Authors

Ibanez Shimabukuro, M., Smith, B.O., Rey Burusco, M., Corsico, B., Kennedy, M.W., Cooper, A.

Assembly

MS1

Entity

1. rAs-p18 (polymer, Thiol state: not present), 155 monomers, 18309.26 Da Detail

MRGSHHHHHH GSKTLPDKFL GTFKLERDEN FDEYLKARGY GWIMRQVIKL AGVTKKFRNA ASGKPDRYDM ENLTTKKDTH HKDWALGEEF QDEALDSTQH KITFDLKDPN TLTETHIKVD DPTDVETYEY RRDGDYLVMK MSWKGVSTSR YYKKQ

2. MS1, entity OLA (non-polymer), 282.461 Da

Total weight

18591.72 Da

Max. entity weight

18309.26 Da

Source organism

Ascaris suum

Exptl. method

solution NMR

Refine. method

simulated annealingsimulated annealing

Data set

assigned_chemical_shifts, spectral_peak_list

Chem. Shift Complete1

Sequence coverage: 96.1 %, Completeness: 91.1 %, Completeness (bb): 92.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	91.1 % (1701 of 1868)	90.7 % (888 of 979)	91.3 % (664 of 727)	92.0 % (149 of 162)
Backbone	92.6 % (854 of 922)	92.1 % (291 of 316)	93.4 % (425 of 455)	91.4 % (138 of 151)
Sidechain	88.9 % (970 of 1091)	89.0 % (590 of 663)	88.7 % (370 of 417)	90.9 % (10 of 11)
Aromatic	72.0 % (144 of 200)	73.0 % (73 of 100)	70.1 % (68 of 97)	100.0 % (3 of 3)
Methyl	96.8 % (120 of 124)	96.8 % (60 of 62)	96.8 % (60 of 62)

1. rAs-p18

MRGSHHHHHH GSKTLPDKFL GTFKLERDEN FDEYLKARGY GWIMRQVIKL AGVTKKFRNA ASGKPDRYDM ENLTTKKDTH HKDWALGEEF QDEALDSTQH KITFDLKDPN TLTETHIKVD DPTDVETYEY RRDGDYLVMK MSWKGVSTSR YYKKQ

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 7.400, Details pH 7.4 298K

#	Name	Isotope labeling	Concentration
5	Oleic Acid	natural abundance	2000.0 mM
6	rAs-p18	[U-99% 13C; U-99% 15N]	500.0 mM
7	Sodium phosphate buffer	natural abundance	20.0 mM

Chem. Shift Complete2

Sequence coverage: 98.1 %, Completeness: 89.3 %, Completeness (bb): 88.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	89.3 % (3336 of 3736)	91.3 % (1787 of 1958)	85.9 % (1249 of 1454)	92.6 % (300 of 324)
Backbone	88.7 % (1635 of 1844)	92.9 % (587 of 632)	84.6 % (770 of 910)	92.1 % (278 of 302)
Sidechain	90.0 % (1964 of 2182)	90.0 % (1193 of 1326)	89.9 % (750 of 834)	95.5 % (21 of 22)
Aromatic	72.5 % (290 of 400)	73.0 % (146 of 200)	71.1 % (138 of 194)	100.0 % (6 of 6)
Methyl	98.4 % (244 of 248)	98.4 % (122 of 124)	98.4 % (122 of 124)

1. rAs-p18

MRGSHHHHHH GSKTLPDKFL GTFKLERDEN FDEYLKARGY GWIMRQVIKL AGVTKKFRNA ASGKPDRYDM ENLTTKKDTH HKDWALGEEF QDEALDSTQH KITFDLKDPN TLTETHIKVD DPTDVETYEY RRDGDYLVMK MSWKGVSTSR YYKKQ

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 7.200, Details pH 7.2 298K

#	Name	Isotope labeling	Type	Concentration
3	rAs-p18	[U-99% 13C; U-99% 15N]		400.0 mM
4	Sodium phosphate buffer	natural abundance		20.0 mM

Protein Blocks Logo

Calculated from 20 models in PDB: 6I9F, Strand ID: A Detail

Release date

2013-02-11

Citation

Resonance assignment of As-p18, a fatty acid binding protein secreted by developing larvae of the parasitic nematode Ascaris suum
Ibanez-Shimabukuro, M., Rey-Burusco, M., Cooper, A., Kennedy, M.W., Corsico, B., Smith, B.O.
Biomol. NMR Assign. (2014), 8, 33-36, PubMed 23225165 , DOI 10.1007/s12104-012-9447-1 , Abstract

Related entities 1. rAs-p18, : 1 : 1 : 198 entities Detail

Experiments performed 15 experiments Detail

1 3D HNCO

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1.000 atm, Temperature 297.700 K, pH 7.400, Details pH 7.4 298K

#	Name	Isotope labeling	Type	Concentration
1	rAs-p18	[U-99% 13C; U-99% 15N]		500.0 mM
2	Sodium phosphate buffer	natural abundance		60.0 mM

2 CBCACONH ({CA|Cca}coNH)

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1.000 atm, Temperature 297.700 K, pH 7.400, Details pH 7.4 298K

#	Name	Isotope labeling	Type	Concentration
1	rAs-p18	[U-99% 13C; U-99% 15N]		500.0 mM
2	Sodium phosphate buffer	natural abundance		60.0 mM

3 3D HNCACB

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1.000 atm, Temperature 297.700 K, pH 7.400, Details pH 7.4 298K

#	Name	Isotope labeling	Type	Concentration
1	rAs-p18	[U-99% 13C; U-99% 15N]		500.0 mM
2	Sodium phosphate buffer	natural abundance		60.0 mM

4 HNCACO (H[N[ca[CO]]])

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1.000 atm, Temperature 297.700 K, pH 7.400, Details pH 7.4 298K

#	Name	Isotope labeling	Type	Concentration
1	rAs-p18	[U-99% 13C; U-99% 15N]		500.0 mM
2	Sodium phosphate buffer	natural abundance		60.0 mM

5 3D HBHA(CO)NH

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1.000 atm, Temperature 297.700 K, pH 7.400, Details pH 7.4 298K

#	Name	Isotope labeling	Type	Concentration
1	rAs-p18	[U-99% 13C; U-99% 15N]		500.0 mM
2	Sodium phosphate buffer	natural abundance		60.0 mM

6 2D 1H-15N HSQC/HMQC

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 7.200, Details pH 7.2 298K

#	Name	Isotope labeling	Type	Concentration
3	rAs-p18	[U-99% 13C; U-99% 15N]		400.0 mM
4	Sodium phosphate buffer	natural abundance		20.0 mM

7 3D HCCH-TOCSY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1.000 atm, Temperature 297.700 K, pH 7.400, Details pH 7.4 298K

#	Name	Isotope labeling	Type	Concentration
1	rAs-p18	[U-99% 13C; U-99% 15N]		500.0 mM
2	Sodium phosphate buffer	natural abundance		60.0 mM

8 3D 1H-13C NOESY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 7.200, Details pH 7.2 298K

#	Name	Isotope labeling	Type	Concentration
3	rAs-p18	[U-99% 13C; U-99% 15N]		400.0 mM
4	Sodium phosphate buffer	natural abundance		20.0 mM

9 2D 1H-13C HSQC/HMQC

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 7.200, Details pH 7.2 298K

#	Name	Isotope labeling	Type	Concentration
3	rAs-p18	[U-99% 13C; U-99% 15N]		400.0 mM
4	Sodium phosphate buffer	natural abundance		20.0 mM

10 2D 1H-15N HSQC/HMQC

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 7.400, Details pH 7.4 298K

#	Name	Isotope labeling	Concentration
5	Oleic Acid	natural abundance	2000.0 mM
6	rAs-p18	[U-99% 13C; U-99% 15N]	500.0 mM
7	Sodium phosphate buffer	natural abundance	20.0 mM

11 Arom32HD (hbCBcgcdHD)

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 7.400, Details pH 7.4 298K

#	Name	Isotope labeling	Concentration
5	Oleic Acid	natural abundance	2000.0 mM
6	rAs-p18	[U-99% 13C; U-99% 15N]	500.0 mM
7	Sodium phosphate buffer	natural abundance	20.0 mM

12 Arom33HE (hbCBcgcdceHE)

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 7.400, Details pH 7.4 298K

#	Name	Isotope labeling	Concentration
5	Oleic Acid	natural abundance	2000.0 mM
6	rAs-p18	[U-99% 13C; U-99% 15N]	500.0 mM
7	Sodium phosphate buffer	natural abundance	20.0 mM

13 3D 1H-15N NOESY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 7.400, Details pH 7.4 298K

#	Name	Isotope labeling	Concentration
5	Oleic Acid	natural abundance	2000.0 mM
6	rAs-p18	[U-99% 13C; U-99% 15N]	500.0 mM
7	Sodium phosphate buffer	natural abundance	20.0 mM

14 mxen HCCH (hC_CH.relayed)

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 7.200, Details pH 7.2 298K

#	Name	Isotope labeling	Type	Concentration
3	rAs-p18	[U-99% 13C; U-99% 15N]		400.0 mM
4	Sodium phosphate buffer	natural abundance		20.0 mM

15 2D 1H-13C HSQC/HMQC

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 7.400, Details pH 7.4 298K

#	Name	Isotope labeling	Concentration
5	Oleic Acid	natural abundance	2000.0 mM
6	rAs-p18	[U-99% 13C; U-99% 15N]	500.0 mM
7	Sodium phosphate buffer	natural abundance	20.0 mM

null

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Found 1 Document (5.256 seconds)

BMRB: 18632

Sample

Sample

Related entities 1. rAs-p18, : 1 : 1 : 198 entities Detail

Experiments performed 15 experiments Detail

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