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BMRB: 18657

2LX2

1H,13C,15N assignments for an isoform of the type III antifreeze protein from notched-fin eelpout

Authors

Kumeta, H., Ogura, K., Nishimiya, Y., Miura, A., Inagaki, F., Tsuda, S.

Assembly

nfeAFP11

Entity

1. nfeAFP11 (polymer, Thiol state: not present), 67 monomers, 7153.466 Da Detail

MNQESVVAAQ LIPINTALTL VMMTTRVVSP TGIPAEDIPR LISMQVNQVV PMGTTLMPDM VKGYAPA

Formula weight

7153.466 Da

Source organism

Zoarces elongatus

Exptl. method

Refine. method

distance geometry, simulated annealing, torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 98.3 %, Completeness (bb): 97.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	98.3 % (738 of 751)	99.5 % (384 of 386)	97.0 % (289 of 298)	97.0 % (65 of 67)
Backbone	97.2 % (377 of 388)	98.5 % (128 of 130)	96.5 % (191 of 198)	96.7 % (58 of 60)
Sidechain	99.5 % (425 of 427)	100.0 % (256 of 256)	98.8 % (162 of 164)	100.0 % (7 of 7)
Aromatic	75.0 % (6 of 8)	100.0 % (4 of 4)	50.0 % (2 of 4)
Methyl	100.0 % (102 of 102)	100.0 % (51 of 51)	100.0 % (51 of 51)

1. nfeAFP11

MNQESVVAAQ LIPINTALTL VMMTTRVVSP TGIPAEDIPR LISMQVNQVV PMGTTLMPDM VKGYAPA

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
1	nfeAFP11	[U-100% 13C; U-100% 15N]		2 mM
2	potassium chloride	natural abundance		20 mM
3	DSS	natural abundance		0.1 mM
4	H2O	natural abundance		90 %
5	D2O	natural abundance		10 %

LACS Plot; CA

Referencing offset: -0.35 ppm, Outliers: 2 Detail

LACS Plot; CB

Referencing offset: -0.35 ppm, Outliers: 2 Detail

LACS Plot; HA

Referencing offset: 0.04 ppm, Outliers: 2 Detail

LACS Plot; CO

Referencing offset: 0.54 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2LX2, Strand ID: A Detail

Release date

2013-02-12

Citation

NMR structure note: a defective isoform and its activity-improved variant of a type III antifreeze protein from Zoarces elongates Kner
Kumeta, H., Ogura, K., Nishimiya, Y., Miura, A., Inagaki, F., Tsuda, S.
J. Biomol. NMR (2013), 55, 225-230, PubMed 23288342 , DOI 10.1007/s10858-012-9703-9 , Abstract

Related entities 1. nfeAFP11, : 1 : 1 : 79 entities Detail

Experiments performed 12 experiments Detail

1 2D 1H-15N HSQC

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
1	nfeAFP11	[U-100% 13C; U-100% 15N]		2 mM
2	potassium chloride	natural abundance		20 mM
3	DSS	natural abundance		0.1 mM
4	H2O	natural abundance		90 %
5	D2O	natural abundance		10 %

2 2D 1H-13C HSQC

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
1	nfeAFP11	[U-100% 13C; U-100% 15N]		2 mM
2	potassium chloride	natural abundance		20 mM
3	DSS	natural abundance		0.1 mM
4	H2O	natural abundance		90 %
5	D2O	natural abundance		10 %

3 3D HNCO

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
1	nfeAFP11	[U-100% 13C; U-100% 15N]		2 mM
2	potassium chloride	natural abundance		20 mM
3	DSS	natural abundance		0.1 mM
4	H2O	natural abundance		90 %
5	D2O	natural abundance		10 %

4 3D HN(CO)CA

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
1	nfeAFP11	[U-100% 13C; U-100% 15N]		2 mM
2	potassium chloride	natural abundance		20 mM
3	DSS	natural abundance		0.1 mM
4	H2O	natural abundance		90 %
5	D2O	natural abundance		10 %

5 3D HNCA

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
1	nfeAFP11	[U-100% 13C; U-100% 15N]		2 mM
2	potassium chloride	natural abundance		20 mM
3	DSS	natural abundance		0.1 mM
4	H2O	natural abundance		90 %
5	D2O	natural abundance		10 %

6 3D HBHA(CO)NH

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
1	nfeAFP11	[U-100% 13C; U-100% 15N]		2 mM
2	potassium chloride	natural abundance		20 mM
3	DSS	natural abundance		0.1 mM
4	H2O	natural abundance		90 %
5	D2O	natural abundance		10 %

7 3D CBCA(CO)NH

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
1	nfeAFP11	[U-100% 13C; U-100% 15N]		2 mM
2	potassium chloride	natural abundance		20 mM
3	DSS	natural abundance		0.1 mM
4	H2O	natural abundance		90 %
5	D2O	natural abundance		10 %

8 3D C(CO)NH

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
1	nfeAFP11	[U-100% 13C; U-100% 15N]		2 mM
2	potassium chloride	natural abundance		20 mM
3	DSS	natural abundance		0.1 mM
4	H2O	natural abundance		90 %
5	D2O	natural abundance		10 %

9 3D HCCH-TOCSY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
1	nfeAFP11	[U-100% 13C; U-100% 15N]		2 mM
2	potassium chloride	natural abundance		20 mM
3	DSS	natural abundance		0.1 mM
4	H2O	natural abundance		90 %
5	D2O	natural abundance		10 %

10 3D 1H-15N NOESY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
1	nfeAFP11	[U-100% 13C; U-100% 15N]		2 mM
2	potassium chloride	natural abundance		20 mM
3	DSS	natural abundance		0.1 mM
4	H2O	natural abundance		90 %
5	D2O	natural abundance		10 %

11 3D 1H-13C NOESY aliphatic

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
1	nfeAFP11	[U-100% 13C; U-100% 15N]		2 mM
2	potassium chloride	natural abundance		20 mM
3	DSS	natural abundance		0.1 mM
4	H2O	natural abundance		90 %
5	D2O	natural abundance		10 %

12 3D 1H-13C NOESY aromatic

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
1	nfeAFP11	[U-100% 13C; U-100% 15N]		2 mM
2	potassium chloride	natural abundance		20 mM
3	DSS	natural abundance		0.1 mM
4	H2O	natural abundance		90 %
5	D2O	natural abundance		10 %

nullKeywords anchored clathrate waters, antifreeze protein, ice-binding, thermal hysteresis