BMRBj - BMREntryMaster

Found 1 Document (1.047 seconds)

BMRB: 18677

2LXH

1H, 13C and 15N Assignments of the RING domain in ubiquitin ligase gp78

Authors

Das, R., Linag, Y., Mariano, J., Li, J., Huang, T., King, A., Weissman, A., Ji, X., Byrd, R.

Assembly

RING domain in ubiquitin ligase gp78

Entity

1. gp78RING (polymer), 58 monomers, 6467.308 Da Detail

AVATPEELAV NNDDCAICWD SMQAARKLPC GHLFHNSCLR SWLEQDTSCP TCRMSLNI

2. ZINC ION (non-polymer), 65.409 × 2 Da

Total weight

6598.126 Da

Max. entity weight

6467.308 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 92.3 %, Completeness (bb): 99.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	92.3 % (591 of 640)	89.7 % (296 of 330)	93.9 % (232 of 247)	100.0 % (63 of 63)
Backbone	99.1 % (339 of 342)	100.0 % (114 of 114)	98.3 % (170 of 173)	100.0 % (55 of 55)
Sidechain	87.0 % (309 of 355)	84.3 % (182 of 216)	90.8 % (119 of 131)	100.0 % (8 of 8)
Aromatic	71.4 % (30 of 42)	76.2 % (16 of 21)	63.2 % (12 of 19)	100.0 % (2 of 2)
Methyl	84.5 % (49 of 58)	86.2 % (25 of 29)	82.8 % (24 of 29)

1. gp78RING

AVATPEELAV NNDDCAICWD SMQAARKLPC GHLFHNSCLR SWLEQDTSCP TCRMSLNI

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.2

#	Name	Isotope labeling	Concentration
1	gp78RING	[U-100% 13C; U-100% 15N]	0.7 ~ 1.0 mM
2	TRIS	natural abundance	50 mM
3	TCEP	natural abundance	2 mM
4	sodium azide	natural abundance	0,2 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.41 ppm, Outliers: 6 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.41 ppm, Outliers: 6 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.05 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.45 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2LXH, Strand ID: C Detail

Release date

2013-08-25

Citation

Allosteric regulation of E2:E3 interactions promote a processive ubiquitination machine
Das, R., Liang, Y., Mariano, J., Li, J., Huang, T., King, A., Tarasov, S.G., Weissman, A.M., Ji, X., Byrd, R.
EMBO J. (2013), 32, 2504-2516, PubMed 23942235 , DOI 10.1038/emboj.2013.174 , Abstract

Related entities 1. gp78RING, : 1 : 6 : 1 : 157 entities Detail

Interaction partners 1. gp78RING, : 33 interactors Detail

More details for 33 interactors identified by 11 citations

Experiments performed 11 experiments Detail

1 2D 1H-15N HSQC