BMRBj - BMREntryMaster

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BMRB: 18678

2LXI

NMR structure of the N-terminal RNA Binding domain 1 (RRM1) of the protein RBM10 from Homo sapiens

Authors

Serrano, P., Geralt, M., Dutta, S., Wuthrich, K.

Assembly

RRM1-RBM10

Entity

1. RRM1-RBM10 (polymer, Thiol state: not present), 91 monomers, 10464.72 Da Detail

SNIVMLRMLP QAATEDDIRG QLQSHGVQAR EVRLMRNKSS GQSRGFAFVE FSHLQDATRW MEANQHSLNI LGQKVSMHYS DPKPKINEDW L

Formula weight

10464.72 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 94.3 %, Completeness (bb): 93.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	94.3 % (1018 of 1079)	96.1 % (546 of 568)	90.9 % (371 of 408)	98.1 % (101 of 103)
Backbone	93.9 % (507 of 540)	98.4 % (181 of 184)	89.6 % (240 of 268)	97.7 % (86 of 88)
Sidechain	95.4 % (596 of 625)	95.1 % (365 of 384)	95.6 % (216 of 226)	100.0 % (15 of 15)
Aromatic	88.5 % (69 of 78)	89.7 % (35 of 39)	86.5 % (32 of 37)	100.0 % (2 of 2)
Methyl	97.6 % (82 of 84)	100.0 % (42 of 42)	95.2 % (40 of 42)

1. entity

SNIVMLRMLP QAATEDDIRG QLQSHGVQAR EVRLMRNKSS GQSRGFAFVE FSHLQDATRW MEANQHSLNI LGQKVSMHYS DPKPKINEDW L

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	RRM1-RBM10	[U-98% 13C; U-98% 15N]	1.2 mM
2	sodium chloride	natural abundance	50 mM
3	sodium phosphate	natural abundance	20 mM
4	sodium azide	natural abundance	5 mM
5	H2O	natural abundance	95 %
6	D2O	natural abundance	5 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.05 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.05 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.04 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.17 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 19 models in PDB: 2LXI, Strand ID: A Detail

Release date

2012-09-16

Citation

NMR structure of the N-terminal RNA Binding domain 1 (RRM1) of the protein RBM10 from Homo sapiens
Serrano, P., Wuthrich, K.
Not known

Related entities 1. RRM1-RBM10, : 1 : 4 : 240 entities Detail

Interaction partners 1. RRM1-RBM10, : 6 interactors Detail

More details for 6 interactors identified by 1 citations

Experiments performed 7 experiments Detail

1 2D 1H-15N HSQC