Backbone 1H, 13C, and 15N Chemical Shift Assignments for cold shock protein, LmCsp with dT7
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 83.4 % (628 of 753) | 83.1 % (325 of 391) | 80.1 % (230 of 287) | 97.3 % (73 of 75) |
Backbone | 94.4 % (372 of 394) | 95.0 % (133 of 140) | 93.1 % (176 of 189) | 96.9 % (63 of 65) |
Sidechain | 74.8 % (311 of 416) | 76.5 % (192 of 251) | 70.3 % (109 of 155) | 100.0 % (10 of 10) |
Aromatic | 9.3 % (8 of 86) | 9.3 % (4 of 43) | 7.1 % (3 of 42) | 100.0 % (1 of 1) |
Methyl | 94.2 % (49 of 52) | 88.5 % (23 of 26) | 100.0 % (26 of 26) |
1. LmCsp dT7
MEQGTVKWFN AEKGFGFIER ENGDDVFVHF SAIQGDGFKS LDEGQAVTFD VEEGQRGPQA ANVQKASolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | LmCsp_dT7 | [U-13C; U-15N] | 0.8 mM | |
2 | potassium phosphate | natural abundance | 50 mM | |
3 | potassium chloride | natural abundance | 100 mM | |
4 | EDTA | natural abundance | 0.1 mM | |
5 | DSS | natural abundance | 0.004 v/v | |
6 | H2O | natural abundance | 90 % | |
7 | D2O | natural abundance | 10 % |