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BMRB: 18681

2LXL

LIP5(MIT)2

Authors

Skalicky, J.J., Sundquist, W.I.

Assembly

LIP5(MIT)2

Entity

1. LIP5(MIT)2 (polymer), 183 monomers, 20829.50 Da Detail

MAALAPLPPL PAQFKSIQHH LRTAQEHDKR DPVVAYYCRL YAMQTGMKID SKTPECRKFL SKLMDQLEAL KKQLGDNEAI TQEIVGCAHL ENYALKMFLY ADNEDRAGRF HKNMIKSFYT ASLLIDVITV FGELTDENVK HRKYARWKAT YIHNCLKNGE TPQAGPVGIE EDNDIEENED AGA

Formula weight

20829.5 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 80.1 %, Completeness (bb): 83.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	80.1 % (1728 of 2156)	86.3 % (972 of 1126)	68.1 % (570 of 837)	96.4 % (186 of 193)
Backbone	83.7 % (906 of 1082)	99.7 % (366 of 367)	68.7 % (371 of 540)	96.6 % (169 of 175)
Sidechain	79.0 % (986 of 1248)	79.8 % (606 of 759)	77.1 % (363 of 471)	94.4 % (17 of 18)
Aromatic	68.3 % (112 of 164)	86.6 % (71 of 82)	49.4 % (40 of 81)	100.0 % (1 of 1)
Methyl	99.5 % (191 of 192)	100.0 % (96 of 96)	99.0 % (95 of 96)

1. LIP5

Show sample condition

Sample

Solvent system 92% H2O/8% D2O, Pressure 1 atm, Temperature 298 K, pH 6.3

#	Name	Isotope labeling	Concentration
1	LIP5(1-183)	[U-100% 13C; U-100% 15N]	0.8 mM
2	sodium phosphate	natural abundance	25 mM
3	sodium chloride	natural abundance	50 mM
4	DTT	natural abundance	0.5 mM
5	EDTA	natural abundance	0.1 mM
6	H2O	natural abundance	92 %
7	D2O	natural abundance	8 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.09 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.09 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.15 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 2LXL, Strand ID: A Detail

Release date

2012-11-26

Citation

Interactions of the human LIP5 regulatory protein with endosomal sorting complexes required for transport
Skalicky, J.J., Arii, J., Wenzel, D.M., Stubblefield, W.B., Katsuyama, A., Uter, N.T., Bajorek, M., Myszka, D.G., Sundquist, W.I.
J. Biol. Chem. (2012), 287, 43910-43926, PubMed 23105106 , DOI 10.1074/jbc.M112.417899 , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 18682 released on 2012-11-26
Title LIP5-CHMP5

Related entities 1. LIP5(MIT)2, : 1 : 2 : 1 : 5 : 21 entities Detail

Interaction partners 1. LIP5(MIT)2, : 26 interactors Detail

More details for 26 interactors identified by 11 citations

Experiments performed 10 experiments Detail

1 3D 1H-15N NOESY