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BMRB: 18682

2LXM

LIP5-CHMP5

Authors

Skalicky, J.J., Sundquist, W.I.

Assembly

LIP5-CHMP5

Entity

1. LIP5(1-168) (polymer), 168 monomers, 19184.95 Da Detail

MAALAPLPPL PAQFKSIQHH LRTAQEHDKR DPVVAYYCRL YAMQTGMKID SKTPECRKFL SKLMDQLEAL KKQLGDNEAI TQEIVGCAHL ENYALKMFLY ADNEDRAGRF HKNMIKSFYT ASLLIDVITV FGELTDENVK HRKYARWKAT YIHNCLKNGE TPQAGPVG

2. CHMP5(139-195) (polymer, Thiol state: not present), 59 monomers, 6294.475 Da Detail

GHMEDANEIQ EALSRSYGTP ELDEDDLEAE LDALGDELLA DEDSSYLDEA ASAPAIPEG

Total weight

25479.424 Da

Max. entity weight

19184.95 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.6 %, Completeness: 79.5 %, Completeness (bb): 82.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	79.5 % (2075 of 2609)	85.8 % (1164 of 1357)	67.1 % (683 of 1018)	97.4 % (228 of 234)
Backbone	82.4 % (1104 of 1340)	98.0 % (446 of 455)	66.8 % (447 of 669)	97.7 % (211 of 216)
Sidechain	79.0 % (1173 of 1484)	79.6 % (718 of 902)	77.7 % (438 of 564)	94.4 % (17 of 18)
Aromatic	60.3 % (111 of 184)	72.8 % (67 of 92)	47.3 % (43 of 91)	100.0 % (1 of 1)
Methyl	100.0 % (240 of 240)	100.0 % (120 of 120)	100.0 % (120 of 120)

1. LIP5(1-168)

MAALAPLPPL PAQFKSIQHH LRTAQEHDKR DPVVAYYCRL YAMQTGMKID SKTPECRKFL SKLMDQLEAL KKQLGDNEAI TQEIVGCAHL ENYALKMFLY ADNEDRAGRF HKNMIKSFYT ASLLIDVITV FGELTDENVK HRKYARWKAT YIHNCLKNGE TPQAGPVG

2. CHMP5(139-195)

GHMEDANEIQ EALSRSYGTP ELDEDDLEAE LDALGDELLA DEDSSYLDEA ASAPAIPEG

Show sample condition

Sample

Solvent system 92% H2O/8% D2O, Pressure 1 atm, Temperature 298 K, pH 6.3

#	Name	Isotope labeling	Concentration
1	LIP5(1-168)	[U-100% 13C; U-100% 15N]	0.5 mM
2	CHMP5(139-195)	[U-100% 13C; U-100% 15N]	0.5 mM
3	sodium phosphate	natural abundance	25 mM
4	sodium chloride	natural abundance	50 mM
5	DTT	natural abundance	0.5 mM
6	EDTA	natural abundance	0.1 mM
7	H2O	natural abundance	92 %
8	D2O	natural abundance	8 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.11 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.11 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.02 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 2LXM, Strand ID: A, B Detail

Release date

2012-11-26

Citation

Interactions of the human LIP5 regulatory protein with endosomal sorting complexes required for transport
Skalicky, J.J., Arii, J., Wenzel, D.M., Stubblefield, W.B., Katsuyama, A., Uter, N.T., Bajorek, M., Myszka, D.G., Sundquist, W.I.
J. Biol. Chem. (2012), 287, 43910-43926, PubMed 23105106 , DOI 10.1074/jbc.M112.417899 , Abstract

Related entities 1. LIP5(1-168), : 1 : 4 : 3 : 4 : 22 entities Detail

Related entities 2. CHMP5(139-195), : 1 : 6 : 24 entities Detail

Interaction partners 1. LIP5(1-168), : 25 : 1 interactors Detail

More details for 26 interactors identified by 11 citations

Interaction partners 2. CHMP5(139-195), : 41 : 1 interactors Detail

More details for 42 interactors identified by 11 citations

Experiments performed 12 experiments Detail

1 3D 1H-13C NOESY aliphatic