BMRB: 18702

RNA BINDING PROTEIN Solution structure of the third KH domain of KSRP in complex with the G-rich target sequence.

Authors

Nicastro, G., Garcia-mayoral, M.F., Hollingworth, D., Kelly, G., Martin, S.R., Briata, P., Gherzi, R., Ramos, A.

Assembly

RNA BINDING PROTEIN Solution structure of the third KH domain of KSRP in complex with the G-rich target sequence.

Entity

1. KH-TYPE SPLICING REGULATORY PROTEIN (polymer, Thiol state: all free), 106 monomers, 10895.33 Da Detail

GAMAYGSRIG GGIDVPVPRH SVGVVIGRSG EMIKKIQNDA GVRIQFKQDD GTGPEKIAHI MGPPDRCEHA ARIINDLLQS LRSGPPGPPG GPGMPPGGRG RGRGQG

2. 5'-R(*AP*GP*GP*GP*UP)-3' (polymer, Thiol state: not present), 5 monomers, 1689.004 Da Detail

AGGGU

Total weight

12584.334 Da

Max. entity weight

10895.33 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

ARIA

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 73.0 %, Completeness: 62.2 %, Completeness (bb): 52.5 % Detail

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Polymer type: polypeptide(L) polyribonucleotide

	Total	1H	13C
All	62.2 % (693 of 1114)	69.1 % (448 of 648)	52.6 % (245 of 466)
Backbone	52.5 % (301 of 573)	60.9 % (154 of 253)	45.9 % (147 of 320)
Sidechain	74.0 % (462 of 624)	74.4 % (294 of 395)	73.4 % (168 of 229)
Aromatic	20.8 % (10 of 48)	38.5 % (10 of 26)	0.0 % (0 of 22)
Methyl	100.0 % (90 of 90)	100.0 % (45 of 45)	100.0 % (45 of 45)

1. KH-TYPE SPLICING REGULATORY PROTEIN

GAMAYGSRIG GGIDVPVPRH SVGVVIGRSG EMIKKIQNDA GVRIQFKQDD GTGPEKIAHI MGPPDRCEHA ARIINDLLQS LRSGPPGPPG GPGMPPGGRG RGRGQG

2. 5'-R(*AP*GP*GP*GP*UP)-3'

AGGGU

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Sample

Solvent system 10% water/90% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 7.400, Details 300 uM

#	Name	Isotope labeling	Type	Concentration
1	KH-TYPE SPLICING REGULATORY PROTEIN	[U-13C; U-15N]		300 uM
2	5'-R(*AP*GP*GP*GP*UP)-3'	[U-13C; U-15N]		300 uM

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LACS Plot; CA

Referencing offset: -0.01 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	HDO	proton	4.754 ppm	internal	indirect	1.0

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LACS Plot; CB

Referencing offset: -0.01 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	HDO	proton	4.754 ppm	internal	indirect	1.0

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LACS Plot; HA

Referencing offset: -0.03 ppm, Outliers: 5 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	HDO	proton	4.754 ppm	internal	indirect	1.0

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Protein Blocks Logo

Calculated from 20 models in PDB: 4B8T, Strand ID: A Detail

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Release date

2012-11-04

Citation 1

Noncanonical G recognition mediates KSRP regulation of let-7 biogenesis
Nicastro, G., Garcia-Mayoral, M., Hollingworth, D., Kelly, G., Martin, S.R., Briata, P., Gherzi, R., Ramos, A.
Nat. Struct. Mol. Biol. (2012), 19, 1282-1286, PubMed 23142982 , DOI 10.1038/nsmb.2427 , Abstract

Show more 1 citaion

Citation 2

The structure of the C-terminal KH domains of KSRP reveals a noncanonical motif important for mRNA degradation
Garcia-mayoral, M.F., Hollingworth, D., Masino, L., Diaz-moreno, I., Kelly, G., Gherzi, R., Chou, C.F., Chen, C.Y., Ramos, A.
Structure (2007), 15, 485-498, PubMed 17437720 , DOI 10.1016/j.str.2007.03.006 , Abstract

Hide non-primary 1 citaion

Related entities 1. KH-TYPE SPLICING REGULATORY PROTEIN, : 1 : 3 : 19 entities Detail

Experiments performed 1 experiments Detail