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BMRB: 18709

2LY4

HMGB1-facilitated p53 DNA binding occurs via HMG-box/p53 transactivation domain interaction and is regulated by the acidic tail

Authors

Rowell, J.P., Simpson, K.L., Stott, K., Watson, M., Thomas, J.O.

Assembly

HMG-box/p53

Entity

1. HMG-box/p53, entity 1 (polymer, Thiol state: all free), 83 monomers, 9680.988 Da Detail

GKGDPKKPRG KMSSYAFFVQ TCREEHKKKH PDASVNFSEF SKKCSERWKT MSAKEKGKFE DMAKADKARY EREMKTYIPP KGE

2. HMG-box/p53, entity 2 (polymer, Thiol state: all free), 93 monomers, 9978.988 Da Detail

MEEPQSDPSV EPPLSQETFS DLWKLLPENN VLSPLPSQAM DDLMLSPDDI EQWFTEDPGP DEAPRMPEAA PPVAPAPAAP TPAAPAPAPS WPL

Total weight

19659.977 Da

Max. entity weight

9978.988 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

DGSA-distance geometry simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.9 %, Completeness: 94.7 %, Completeness (bb): 95.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	94.7 % (1950 of 2060)	94.5 % (1038 of 1098)	94.0 % (753 of 801)	98.8 % (159 of 161)
Backbone	95.3 % (955 of 1002)	97.0 % (321 of 331)	93.3 % (487 of 522)	98.7 % (147 of 149)
Sidechain	94.0 % (1154 of 1228)	93.0 % (713 of 767)	95.8 % (430 of 449)	91.7 % (11 of 12)
Aromatic	100.0 % (150 of 150)	100.0 % (75 of 75)	100.0 % (71 of 71)	100.0 % (4 of 4)
Methyl	99.1 % (111 of 112)	98.2 % (55 of 56)	100.0 % (56 of 56)

1. entity 1

GKGDPKKPRG KMSSYAFFVQ TCREEHKKKH PDASVNFSEF SKKCSERWKT MSAKEKGKFE DMAKADKARY EREMKTYIPP KGE

2. entity 2

MEEPQSDPSV EPPLSQETFS DLWKLLPENN VLSPLPSQAM DDLMLSPDDI EQWFTEDPGP DEAPRMPEAA PPVAPAPAAP TPAAPAPAPS WPL

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	entity_1	natural abundance	0.1 ~ 1.0 mM
2	entity_2	natural abundance	0.1 ~ 1.0 mM
3	sodium phosphate	natural abundance	10 mM
4	EDTA	natural abundance	1 mM
5	DTT	natural abundance	1 mM
6	PMSF	natural abundance	0.5 mM
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.03 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.03 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.06 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.3 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 2LY4, Strand ID: A, B Detail

Release date

2012-10-28

Citation

HMGB1-facilitated p53 DNA binding occurs via HMG-Box/p53 transactivation domain interaction, regulated by the acidic tail
Rowell, J.P., Simpson, K.L., Stott, K., Watson, M., Thomas, J.O.
Structure (2012), 20, 2014-2024, PubMed 23063560 , DOI 10.1016/j.str.2012.09.004 , Abstract

Related entities 1. HMG-box/p53, entity 1, : 10 : 1 : 3 : 241 entities Detail

Related entities 2. HMG-box/p53, entity 2, : 1 : 1 entities Detail

Interaction partners 1. HMG-box/p53, entity 1, : 59 interactors Detail

More details for 59 interactors identified by 31 citations

Experiments performed 12 experiments Detail

1 2D 1H-15N HSQC