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BMRB: 18712

2LY7

b-flap domain of RNA polymerase (B. subtilis)

Authors

Mobli, M.

Assembly

b-flap domain of RNA polymerase (B. subtilis)

Entity

1. b-flap domain of RNA polymerase (B. subtilis) (polymer, Thiol state: not present), 149 monomers, 16649.34 Da Detail

MDDVYTSIHI EEYESEARDT KLGPEEITRD IPNVGEDALR NLDDRGVIRI GAEVKDGDLL VGKVTPKGVT ELTAEERLLH AIFGEKAREV RDTSLRVPHG GGGIIHDVKV FNREDGDELP PGVNQLVRVY IVQKRKISEG DGTHHHHHH

Formula weight

16649.34 Da

Source organism

Bacillus subtilis

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.0 %, Completeness: 78.4 %, Completeness (bb): 77.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	78.4 % (1317 of 1680)	84.8 % (741 of 874)	66.5 % (437 of 657)	93.3 % (139 of 149)
Backbone	77.6 % (684 of 882)	91.9 % (283 of 308)	62.2 % (268 of 431)	93.0 % (133 of 143)
Sidechain	81.4 % (758 of 931)	80.9 % (458 of 566)	81.9 % (294 of 359)	100.0 % (6 of 6)
Aromatic	46.4 % (39 of 84)	57.1 % (24 of 42)	35.7 % (15 of 42)
Methyl	92.0 % (162 of 176)	93.2 % (82 of 88)	90.9 % (80 of 88)

1. b-flap

MDDVYTSIHI EEYESEARDT KLGPEEITRD IPNVGEDALR NLDDRGVIRI GAEVKDGDLL VGKVTPKGVT ELTAEERLLH AIFGEKAREV RDTSLRVPHG GGGIIHDVKV FNREDGDELP PGVNQLVRVY IVQKRKISEG DGTHHHHHH

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	b-flap	[U-100% 13C; U-100% 15N]	500 (±50.0) uM
2	potassium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	150 mM
4	H2O	natural abundance	95 %
5	D2O	natural abundance	5 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.773 ppm	internal	indirect	0.2514495
¹H	water	protons	4.773 ppm	internal	direct	1.0
¹⁵N	water	protons	4.773 ppm	internal	indirect	0.1013291

Referencing offset: -0.21 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.773 ppm	internal	indirect	0.2514495
¹H	water	protons	4.773 ppm	internal	direct	1.0
¹⁵N	water	protons	4.773 ppm	internal	indirect	0.1013291

Referencing offset: -0.21 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.773 ppm	internal	indirect	0.2514495
¹H	water	protons	4.773 ppm	internal	direct	1.0
¹⁵N	water	protons	4.773 ppm	internal	indirect	0.1013291

Referencing offset: -0.05 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2LY7, Strand ID: A Detail

Release date

2014-03-09

Citation

NusA binding to the b-flap tip of RNA polymerase determined by NMR
Mobli, M., Yang, X., Ma, C., Thomas, R., King, G., Lewis, P.
Not known

Related entities 1. b-flap domain of RNA polymerase (B. subtilis), : 1 : 206 entities Detail

Interaction partners 1. b-flap domain of RNA polymerase (B. subtilis), : 10 interactors Detail

More details for 10 interactors identified by 4 citations

Experiments performed 10 experiments Detail

1 2D 1H-15N HSQC