BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	80.9 % (1322 of 1634)	75.4 % (657 of 871)	86.6 % (547 of 632)	90.1 % (118 of 131)
Backbone	96.3 % (736 of 764)	95.3 % (244 of 256)	96.9 % (374 of 386)	96.7 % (118 of 122)
Sidechain	71.2 % (709 of 996)	67.3 % (414 of 615)	79.3 % (295 of 372)	0.0 % (0 of 9)
Aromatic	60.0 % (60 of 100)	72.0 % (36 of 50)	49.0 % (24 of 49)	0.0 % (0 of 1)
Methyl	96.3 % (131 of 136)	98.5 % (67 of 68)	94.1 % (64 of 68)

1. Ska1-MTBD

MNDIRIVPQI TDEEFKTIPK YQLGRLTLEM MNEIVSKMDD FLMKKSKILG KTNKQLTRSD REVLDNWREL EMKARKRLPT TLFFIETDIR PMLQDRLRPS FAKAIPCLRH IRRIREERCG PLTFYYPGSS

Show sample condition

Sample #1

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	Ska1-MTBD	[U-100% 15N ILV-Methyl 13C]	0.800 mM
2	potassium phosphate	natural abundance	20 mM
3	NaCl	natural abundance	150 mM
4	DTT	natural abundance	1.5 mM
5	H2O	natural abundance	55 mM
6	sodium azide	natural abundance	0.0001 mM
7	D2O	natural abundance	100 %

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
8	Ska1-MTBD	[U-100% 13C; U-100% 15N]	0.800 mM
9	potassium phosphate	natural abundance	20 mM
10	NaCl	natural abundance	150 mM
11	DTT	natural abundance	1.5 mM
12	H2O	natural abundance	55 mM
13	sodium azide	natural abundance	0.00001 mM
14	H2O	natural abundance	95 %
15	D2O	natural abundance	5 %

Sample #3

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
16	Ska1-MTBD	[U-100% 15N; ILV-Methyl 13C]	0.800 mM
17	potassium phosphate	natural abundance	20 mM
18	NaCl	natural abundance	150 mM
19	DTT	natural abundance	1.5 mM
20	H2O	natural abundance	55 mM
21	sodium azide	natural abundance	0.00001 mM
22	H2O	natural abundance	95 %
23	D2O	natural abundance	5 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.08 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.08 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.12 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2LYC, Strand ID: A Detail

Release date

2012-10-21

Citation

The kinetochore-bound Ska1 complex tracks depolymerizing microtubules and binds to curved protofilaments
Schmidt, J.C., Arthanari, H., Boeszoermenyi, A., Dashkevich, N.M., Wilson-Kubalek, E.M., Monnier, N., Markus, M., Oberer, M., Milligan, R.A., Bathe, M., Wagner, G., Grishchuk, E.L., Cheeseman, I.M.
Dev. Cell (2012), 23, 968-980, PubMed 23085020 , DOI 10.1016/j.devcel.2012.09.012 , Abstract

Related entities 1. Ska1-MTBD, : 1 : 2 : 27 entities Detail

Interaction partners 1. Ska1-MTBD, : 2 interactors Detail

More details for 2 interactors identified by 1 citations

Experiments performed 15 experiments Detail

1 2D 1H-15N HSQC

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
8	Ska1-MTBD	[U-100% 13C; U-100% 15N]	0.800 mM
9	potassium phosphate	natural abundance	20 mM
10	NaCl	natural abundance	150 mM
11	DTT	natural abundance	1.5 mM
12	H2O	natural abundance	55 mM
13	sodium azide	natural abundance	0.00001 mM
14	H2O	natural abundance	95 %
15	D2O	natural abundance	5 %

2 2D 1H-13C HSQC

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
8	Ska1-MTBD	[U-100% 13C; U-100% 15N]	0.800 mM
9	potassium phosphate	natural abundance	20 mM
10	NaCl	natural abundance	150 mM
11	DTT	natural abundance	1.5 mM
12	H2O	natural abundance	55 mM
13	sodium azide	natural abundance	0.00001 mM
14	H2O	natural abundance	95 %
15	D2O	natural abundance	5 %

3 3D HNCO

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
8	Ska1-MTBD	[U-100% 13C; U-100% 15N]	0.800 mM
9	potassium phosphate	natural abundance	20 mM
10	NaCl	natural abundance	150 mM
11	DTT	natural abundance	1.5 mM
12	H2O	natural abundance	55 mM
13	sodium azide	natural abundance	0.00001 mM
14	H2O	natural abundance	95 %
15	D2O	natural abundance	5 %

4 3D HNCA

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
8	Ska1-MTBD	[U-100% 13C; U-100% 15N]	0.800 mM
9	potassium phosphate	natural abundance	20 mM
10	NaCl	natural abundance	150 mM
11	DTT	natural abundance	1.5 mM
12	H2O	natural abundance	55 mM
13	sodium azide	natural abundance	0.00001 mM
14	H2O	natural abundance	95 %
15	D2O	natural abundance	5 %

5 3D HNCACB

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
8	Ska1-MTBD	[U-100% 13C; U-100% 15N]	0.800 mM
9	potassium phosphate	natural abundance	20 mM
10	NaCl	natural abundance	150 mM
11	DTT	natural abundance	1.5 mM
12	H2O	natural abundance	55 mM
13	sodium azide	natural abundance	0.00001 mM
14	H2O	natural abundance	95 %
15	D2O	natural abundance	5 %

6 3D HN(CO)CA

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
8	Ska1-MTBD	[U-100% 13C; U-100% 15N]	0.800 mM
9	potassium phosphate	natural abundance	20 mM
10	NaCl	natural abundance	150 mM
11	DTT	natural abundance	1.5 mM
12	H2O	natural abundance	55 mM
13	sodium azide	natural abundance	0.00001 mM
14	H2O	natural abundance	95 %
15	D2O	natural abundance	5 %

7 3D HCACO

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
8	Ska1-MTBD	[U-100% 13C; U-100% 15N]	0.800 mM
9	potassium phosphate	natural abundance	20 mM
10	NaCl	natural abundance	150 mM
11	DTT	natural abundance	1.5 mM
12	H2O	natural abundance	55 mM
13	sodium azide	natural abundance	0.00001 mM
14	H2O	natural abundance	95 %
15	D2O	natural abundance	5 %

8 3D 1H-15N NOESY

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
8	Ska1-MTBD	[U-100% 13C; U-100% 15N]	0.800 mM
9	potassium phosphate	natural abundance	20 mM
10	NaCl	natural abundance	150 mM
11	DTT	natural abundance	1.5 mM
12	H2O	natural abundance	55 mM
13	sodium azide	natural abundance	0.00001 mM
14	H2O	natural abundance	95 %
15	D2O	natural abundance	5 %

9 3D time shared 13C-15N NOESY

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
16	Ska1-MTBD	[U-100% 15N; ILV-Methyl 13C]	0.800 mM
17	potassium phosphate	natural abundance	20 mM
18	NaCl	natural abundance	150 mM
19	DTT	natural abundance	1.5 mM
20	H2O	natural abundance	55 mM
21	sodium azide	natural abundance	0.00001 mM
22	H2O	natural abundance	95 %
23	D2O	natural abundance	5 %

10 4D HMQC-NOESY-HMQC

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	Ska1-MTBD	[U-100% 15N ILV-Methyl 13C]	0.800 mM
2	potassium phosphate	natural abundance	20 mM
3	NaCl	natural abundance	150 mM
4	DTT	natural abundance	1.5 mM
5	H2O	natural abundance	55 mM
6	sodium azide	natural abundance	0.0001 mM
7	D2O	natural abundance	100 %

11 2D-HBCBCGCDHD

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
8	Ska1-MTBD	[U-100% 13C; U-100% 15N]	0.800 mM
9	potassium phosphate	natural abundance	20 mM
10	NaCl	natural abundance	150 mM
11	DTT	natural abundance	1.5 mM
12	H2O	natural abundance	55 mM
13	sodium azide	natural abundance	0.00001 mM
14	H2O	natural abundance	95 %
15	D2O	natural abundance	5 %

12 2D-HBCBCGCDCEHE

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
8	Ska1-MTBD	[U-100% 13C; U-100% 15N]	0.800 mM
9	potassium phosphate	natural abundance	20 mM
10	NaCl	natural abundance	150 mM
11	DTT	natural abundance	1.5 mM
12	H2O	natural abundance	55 mM
13	sodium azide	natural abundance	0.00001 mM
14	H2O	natural abundance	95 %
15	D2O	natural abundance	5 %

13 3D C(CO)NH

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
8	Ska1-MTBD	[U-100% 13C; U-100% 15N]	0.800 mM
9	potassium phosphate	natural abundance	20 mM
10	NaCl	natural abundance	150 mM
11	DTT	natural abundance	1.5 mM
12	H2O	natural abundance	55 mM
13	sodium azide	natural abundance	0.00001 mM
14	H2O	natural abundance	95 %
15	D2O	natural abundance	5 %

14 3D H(CCO)NH

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
8	Ska1-MTBD	[U-100% 13C; U-100% 15N]	0.800 mM
9	potassium phosphate	natural abundance	20 mM
10	NaCl	natural abundance	150 mM
11	DTT	natural abundance	1.5 mM
12	H2O	natural abundance	55 mM
13	sodium azide	natural abundance	0.00001 mM
14	H2O	natural abundance	95 %
15	D2O	natural abundance	5 %

15 3D HCCH-TOCSY

Instrument

Varian INOVA - 500 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
8	Ska1-MTBD	[U-100% 13C; U-100% 15N]	0.800 mM
9	potassium phosphate	natural abundance	20 mM
10	NaCl	natural abundance	150 mM
11	DTT	natural abundance	1.5 mM
12	H2O	natural abundance	55 mM
13	sodium azide	natural abundance	0.00001 mM
14	H2O	natural abundance	95 %
15	D2O	natural abundance	5 %

NMR combined restraints 4 contents Detail

Properties

Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints	combined_18717_2lyc.nef
Input source #2: Coordindates	2lyc.cif
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Sequence alignments

Entity_assembly_ID (NMR): A vs Auth_asym_ID (model): A

--------10--------20--------30--------40--------50--------60--------70--------80--------90-------100
MNDIRIVPQITDEEFKTIPKYQLGRLTLEMMNEIVSKMDDFLMKKSKILGKTNKQLTRSDREVLDNWRELEMKARKRLPTTLFFIETDIRPMLQDRLRPS
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
MNDIRIVPQITDEEFKTIPKYQLGRLTLEMMNEIVSKMDDFLMKKSKILGKTNKQLTRSDREVLDNWRELEMKARKRLPTTLFFIETDIRPMLQDRLRPS

-------110-------120-------130
FAKAIPCLRHIRRIREERCGPLTFYYPGSS
||||||||||||||||||||||||||||||
FAKAIPCLRHIRRIREERCGPLTFYYPGSS

Chain assignments

Entity_assembly_ID (NMR)	Auth_asym_ID (model)	Length	Unmapped	Conflict	Sequence coverage (%)
A	A	130	0	0	100.0

Content subtype: combined_18717_2lyc.nef

#	Content subtype	Saveframe	Status	# of rows (sets)	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	1110		93.1 (chain: A, length: 130)
1	Distance restraints	DYANA/DIANA_distance_constraints_2	Warning	1971 (1)	noe	95.4 (chain: A, length: 130)
2	Distance restraints	DYANA/DIANA_distance_constraints_3	Warning	97 (1)	hbond	43.1 (chain: A, length: 130)

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 130, Sequence coverage: 93.1%
- Total number of assignments
  - ¹H chemical shifts: 614
  - ¹³C chemical shifts: 380
  - ¹⁵N chemical shifts: 116
- Completeness of assignments
  - Entity_assemble_ID: A
- Redox state of CYS
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: A

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	871	597	68.5
¹³C chemical shifts	632	379	60.0
¹⁵N chemical shifts	146	116	79.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	256	240	93.8
¹³C chemical shifts	260	119	45.8
¹⁵N chemical shifts	122	116	95.1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	615	357	58.0
¹³C chemical shifts	372	260	69.9
¹⁵N chemical shifts	24	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	75	62	82.7
¹³C chemical shifts	75	60	80.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	50	30	60.0
¹³C chemical shifts	49	26	53.1
¹⁵N chemical shifts	1	0	0.0

Distance restraints

Saveframe: DYANA/DIANA_distance_constraints_2
- Status: Warning
- Experiment type: noe
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 130, Sequence coverage: 95.4%
- Total number of restraints: 1971
- Weight of restraints: 1.0 (1971)
- Potential type of restraints: upper-bound-parabolic (1971)
- Range of distance target values: 2.71, 8.0 (Å)
- Histogram of distance target values
- Distance restraints per residue
  - Chain_ID: A
- Distance restraints on contact map
- Saveframe: DYANA/DIANA_distance_constraints_3
  - Status: Warning
  - Experiment type: hbond
  - Sequence coverage of experimental data
    - Entity_assembly_ID: A, Chain length: 130, Sequence coverage: 43.1%
  - Total number of restraints: 97
  - Weight of restraints: 1.0 (97)
  - Potential type of restraints: upper-bound-parabolic (97)
  - Range of distance target values: 2.4, 3.93 (Å)
  - Histogram of distance target values
  - Distance restraints per residue
    - Chain_ID: A
  - Distance restraints on contact map

Keywords kinetochore, microtubule, Ska1, Ska1-MTBD

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Found 1 Document (0.771 seconds)

BMRB: 18717

Sample #1

Sample #2

Sample #3

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. Ska1-MTBD, : 1 : 2 : 27 entities Detail

Interaction partners 1. Ska1-MTBD, : 2 interactors Detail

Experiments performed 15 experiments Detail

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NMR combined restraints 4 contents Detail