BMRBj - BMREntryMaster

Found 1 Document (7.711 seconds)

BMRB: 18733

2LYY

NMR structure of the protein NB7890A from Shewanella sp

Authors

Serrano, P., Geralt, M., Pedrini, B., Wuthrich, K., Horst, R., Augustyniak, W.

Assembly

NB7890A

Entity

1. NB7890A (polymer, Thiol state: not present), 208 monomers, 24955.24 Da Detail

SNNENAFYAR ATELIKLANQ QNQNTEIQTG EVSASFMWAL ARYNAWFGST SFETKEQMQA KKQEMMDYYM DRYKEMLDAN MEDYIENFDH YRATQKMEDY IENFDHYRAT QKSNNENAFY ARATELIKLA NQQNQNTEIQ TGEVSASFMW ALARYNAWFG STSFETKEQM QAKKQEMMDY YMDRYKEMLD ANMEDYIENF DHYRATQK

Formula weight

24955.24 Da

Source organism

Shewanella sp.

Exptl. method

solution NMR

Refine. method

molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 59.6 %, Completeness: 51.7 %, Completeness (bb): 50.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	51.7 % (1303 of 2520)	55.3 % (730 of 1319)	45.0 % (429 of 953)	58.1 % (144 of 248)
Backbone	50.0 % (624 of 1248)	57.6 % (242 of 420)	42.1 % (261 of 620)	58.2 % (121 of 208)
Sidechain	53.9 % (795 of 1476)	54.3 % (488 of 899)	52.9 % (284 of 537)	57.5 % (23 of 40)
Aromatic	54.0 % (161 of 298)	57.0 % (85 of 149)	49.7 % (72 of 145)	100.0 % (4 of 4)
Methyl	58.6 % (82 of 140)	58.6 % (41 of 70)	58.6 % (41 of 70)

1. NB7890A

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	NB7890A	[U-98% 13C; U-98% 15N]	1.2 mM
2	sodium chloride	natural abundance	50 mM
3	sodium phosphate	natural abundance	20 mM
4	sodium azide	natural abundance	5 mM
5	H2O	natural abundance	95 %
6	D2O	natural abundance	5 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.35 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.35 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.08 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2LYY, Strand ID: A, B Detail

Release date

2012-10-21

Citation

Structural Elucidation of a Novel Lipooligosaccharide from the Antarctic Bacterium OMVs Producer Shewanella sp. HM13
Serrano, P., Wuthrich, K.
Mar. Drugs (2019), 17, PubMed 30626008 , DOI 10.3390/md17010034 , Abstract

Related entities 1. NB7890A, : 1 entities Detail

Experiments performed 7 experiments Detail

1 2D 1H-15N HSQC