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BMRB: 18763

2LZL

FGFR3tm

Authors

Lesovoy, D.M., Bocharov, E.V., Goncharuk, S.A., Arseniev, A.S.

Assembly

FGFR3tm

Entity

1. FGFR3tm (polymer, Thiol state: all free), 43 monomers, 4602.305 × 2 Da Detail

LPAEEELVEA DEAGSVYAGI LSYGVGFFLF ILVVAAVTLC RLR

Total weight

9204.61 Da

Max. entity weight

4602.305 Da

Source organism

Exptl. method

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 93.8 %, Completeness (bb): 96.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	93.8 % (452 of 482)	93.8 % (226 of 241)	93.0 % (185 of 199)	97.6 % (41 of 42)
Backbone	96.1 % (246 of 256)	94.4 % (84 of 89)	96.8 % (121 of 125)	97.6 % (41 of 42)
Sidechain	92.5 % (245 of 265)	93.4 % (142 of 152)	91.2 % (103 of 113)
Aromatic	78.3 % (36 of 46)	100.0 % (23 of 23)	56.5 % (13 of 23)
Methyl	100.0 % (74 of 74)	100.0 % (37 of 37)	100.0 % (37 of 37)

1. FGFR3tm

LPAEEELVEA DEAGSVYAGI LSYGVGFFLF ILVVAAVTLC RLR

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 K, pH 5.7

#	Name	Isotope labeling	Type	Concentration
1	FGFR3tm	[U-99% 13C; U-99% 15N]		0.75 mM
2	FGFR3tm	natural abundance		0.75 mM
3	DPC	[U-99% 2H]		88 mM
4	SDS	[U-99% 2H]		10 mM
5	sodium azide	natural abundance		0.3 mM
6	TCEP	natural abundance		6 mM
7	citric acid	natural abundance		5 mM
8	Na2HPO4	natural abundance		15 mM
9	H2O	natural abundance		95 %
10	D2O	natural abundance		5 %

Protein Blocks Logo

Calculated from 25 models in PDB: 2LZL, Strand ID: A, B Detail

Release date

2013-10-03

Citation

Structure of FGFR3 transmembrane domain dimer: implications for signaling and human pathologies
Bocharov, E.V., Lesovoy, D.M., Goncharuk, S.A., Goncharuk, M.V., Hristova, K., Arseniev, A.S.
Structure (2013), 21, 2087-2093, PubMed 24120763 , DOI 10.1016/j.str.2013.08.026 , Abstract

Related entities 1. FGFR3tm, : 1 : 2 : 21 entities Detail

Interaction partners 1. FGFR3tm, : 29 interactors Detail

More details for 29 interactors identified by 9 citations

Experiments performed 14 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 K, pH 5.7

#	Name	Isotope labeling	Type	Concentration
1	FGFR3tm	[U-99% 13C; U-99% 15N]		0.75 mM
2	FGFR3tm	natural abundance		0.75 mM
3	DPC	[U-99% 2H]		88 mM
4	SDS	[U-99% 2H]		10 mM
5	sodium azide	natural abundance		0.3 mM
6	TCEP	natural abundance		6 mM
7	citric acid	natural abundance		5 mM
8	Na2HPO4	natural abundance		15 mM
9	H2O	natural abundance		95 %
10	D2O	natural abundance		5 %

2 2D 1H-15N TROSY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 K, pH 5.7

#	Name	Isotope labeling	Type	Concentration
1	FGFR3tm	[U-99% 13C; U-99% 15N]		0.75 mM
2	FGFR3tm	natural abundance		0.75 mM
3	DPC	[U-99% 2H]		88 mM
4	SDS	[U-99% 2H]		10 mM
5	sodium azide	natural abundance		0.3 mM
6	TCEP	natural abundance		6 mM
7	citric acid	natural abundance		5 mM
8	Na2HPO4	natural abundance		15 mM
9	H2O	natural abundance		95 %
10	D2O	natural abundance		5 %

3 2D 1H-13C constant time HSQC aliphatic

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 K, pH 5.7

#	Name	Isotope labeling	Type	Concentration
1	FGFR3tm	[U-99% 13C; U-99% 15N]		0.75 mM
2	FGFR3tm	natural abundance		0.75 mM
3	DPC	[U-99% 2H]		88 mM
4	SDS	[U-99% 2H]		10 mM
5	sodium azide	natural abundance		0.3 mM
6	TCEP	natural abundance		6 mM
7	citric acid	natural abundance		5 mM
8	Na2HPO4	natural abundance		15 mM
9	H2O	natural abundance		95 %
10	D2O	natural abundance		5 %

4 2D 1H-13C constant time HSQC aromatic

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 K, pH 5.7

#	Name	Isotope labeling	Type	Concentration
1	FGFR3tm	[U-99% 13C; U-99% 15N]		0.75 mM
2	FGFR3tm	natural abundance		0.75 mM
3	DPC	[U-99% 2H]		88 mM
4	SDS	[U-99% 2H]		10 mM
5	sodium azide	natural abundance		0.3 mM
6	TCEP	natural abundance		6 mM
7	citric acid	natural abundance		5 mM
8	Na2HPO4	natural abundance		15 mM
9	H2O	natural abundance		95 %
10	D2O	natural abundance		5 %

5 3D HNCO

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 K, pH 5.7

#	Name	Isotope labeling	Type	Concentration
1	FGFR3tm	[U-99% 13C; U-99% 15N]		0.75 mM
2	FGFR3tm	natural abundance		0.75 mM
3	DPC	[U-99% 2H]		88 mM
4	SDS	[U-99% 2H]		10 mM
5	sodium azide	natural abundance		0.3 mM
6	TCEP	natural abundance		6 mM
7	citric acid	natural abundance		5 mM
8	Na2HPO4	natural abundance		15 mM
9	H2O	natural abundance		95 %
10	D2O	natural abundance		5 %

6 3D HNCA

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 K, pH 5.7

#	Name	Isotope labeling	Type	Concentration
1	FGFR3tm	[U-99% 13C; U-99% 15N]		0.75 mM
2	FGFR3tm	natural abundance		0.75 mM
3	DPC	[U-99% 2H]		88 mM
4	SDS	[U-99% 2H]		10 mM
5	sodium azide	natural abundance		0.3 mM
6	TCEP	natural abundance		6 mM
7	citric acid	natural abundance		5 mM
8	Na2HPO4	natural abundance		15 mM
9	H2O	natural abundance		95 %
10	D2O	natural abundance		5 %

7 3D HN(CO)CA

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 K, pH 5.7

#	Name	Isotope labeling	Type	Concentration
1	FGFR3tm	[U-99% 13C; U-99% 15N]		0.75 mM
2	FGFR3tm	natural abundance		0.75 mM
3	DPC	[U-99% 2H]		88 mM
4	SDS	[U-99% 2H]		10 mM
5	sodium azide	natural abundance		0.3 mM
6	TCEP	natural abundance		6 mM
7	citric acid	natural abundance		5 mM
8	Na2HPO4	natural abundance		15 mM
9	H2O	natural abundance		95 %
10	D2O	natural abundance		5 %

8 3D HNHA

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 K, pH 5.7

#	Name	Isotope labeling	Type	Concentration
1	FGFR3tm	[U-99% 13C; U-99% 15N]		0.75 mM
2	FGFR3tm	natural abundance		0.75 mM
3	DPC	[U-99% 2H]		88 mM
4	SDS	[U-99% 2H]		10 mM
5	sodium azide	natural abundance		0.3 mM
6	TCEP	natural abundance		6 mM
7	citric acid	natural abundance		5 mM
8	Na2HPO4	natural abundance		15 mM
9	H2O	natural abundance		95 %
10	D2O	natural abundance		5 %

9 3D HNHB

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 K, pH 5.7

#	Name	Isotope labeling	Type	Concentration
1	FGFR3tm	[U-99% 13C; U-99% 15N]		0.75 mM
2	FGFR3tm	natural abundance		0.75 mM
3	DPC	[U-99% 2H]		88 mM
4	SDS	[U-99% 2H]		10 mM
5	sodium azide	natural abundance		0.3 mM
6	TCEP	natural abundance		6 mM
7	citric acid	natural abundance		5 mM
8	Na2HPO4	natural abundance		15 mM
9	H2O	natural abundance		95 %
10	D2O	natural abundance		5 %

10 3D HCCH-TOCSY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 K, pH 5.7

#	Name	Isotope labeling	Type	Concentration
1	FGFR3tm	[U-99% 13C; U-99% 15N]		0.75 mM
2	FGFR3tm	natural abundance		0.75 mM
3	DPC	[U-99% 2H]		88 mM
4	SDS	[U-99% 2H]		10 mM
5	sodium azide	natural abundance		0.3 mM
6	TCEP	natural abundance		6 mM
7	citric acid	natural abundance		5 mM
8	Na2HPO4	natural abundance		15 mM
9	H2O	natural abundance		95 %
10	D2O	natural abundance		5 %

11 3D 1H-15N NOESY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 K, pH 5.7

#	Name	Isotope labeling	Type	Concentration
1	FGFR3tm	[U-99% 13C; U-99% 15N]		0.75 mM
2	FGFR3tm	natural abundance		0.75 mM
3	DPC	[U-99% 2H]		88 mM
4	SDS	[U-99% 2H]		10 mM
5	sodium azide	natural abundance		0.3 mM
6	TCEP	natural abundance		6 mM
7	citric acid	natural abundance		5 mM
8	Na2HPO4	natural abundance		15 mM
9	H2O	natural abundance		95 %
10	D2O	natural abundance		5 %

12 3D 1H-13C NOESY aliphatic

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 K, pH 5.7

#	Name	Isotope labeling	Type	Concentration
1	FGFR3tm	[U-99% 13C; U-99% 15N]		0.75 mM
2	FGFR3tm	natural abundance		0.75 mM
3	DPC	[U-99% 2H]		88 mM
4	SDS	[U-99% 2H]		10 mM
5	sodium azide	natural abundance		0.3 mM
6	TCEP	natural abundance		6 mM
7	citric acid	natural abundance		5 mM
8	Na2HPO4	natural abundance		15 mM
9	H2O	natural abundance		95 %
10	D2O	natural abundance		5 %

13 3D 1H-13C NOESY aromatic

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 K, pH 5.7

#	Name	Isotope labeling	Type	Concentration
1	FGFR3tm	[U-99% 13C; U-99% 15N]		0.75 mM
2	FGFR3tm	natural abundance		0.75 mM
3	DPC	[U-99% 2H]		88 mM
4	SDS	[U-99% 2H]		10 mM
5	sodium azide	natural abundance		0.3 mM
6	TCEP	natural abundance		6 mM
7	citric acid	natural abundance		5 mM
8	Na2HPO4	natural abundance		15 mM
9	H2O	natural abundance		95 %
10	D2O	natural abundance		5 %

14 15N,13C-F1-filtered/F3-edited-NOESY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 K, pH 5.7

#	Name	Isotope labeling	Type	Concentration
1	FGFR3tm	[U-99% 13C; U-99% 15N]		0.75 mM
2	FGFR3tm	natural abundance		0.75 mM
3	DPC	[U-99% 2H]		88 mM
4	SDS	[U-99% 2H]		10 mM
5	sodium azide	natural abundance		0.3 mM
6	TCEP	natural abundance		6 mM
7	citric acid	natural abundance		5 mM
8	Na2HPO4	natural abundance		15 mM
9	H2O	natural abundance		95 %
10	D2O	natural abundance		5 %

NMR combined restraints 3 contents Detail

Properties

Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints	combined_18763_2lzl.nef
Input source #2: Coordindates	2lzl.cif
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Sequence alignments

Entity_assembly_ID (NMR): A vs Auth_asym_ID (model): A

---360-----370-------380-------390---------
LPAEEELVEADEAGSVYAGILSYGVGFFLFILVVAAVTLCRLR
|||||||||||||||||||||||||||||||||||||||||||
LPAEEELVEADEAGSVYAGILSYGVGFFLFILVVAAVTLCRLR
--------10--------20--------30--------40---

Entity_assembly_ID (NMR): B vs Auth_asym_ID (model): B

---360-----370-------380-------390---------
LPAEEELVEADEAGSVYAGILSYGVGFFLFILVVAAVTLCRLR
|||||||||||||||||||||||||||||||||||||||||||
LPAEEELVEADEAGSVYAGILSYGVGFFLFILVVAAVTLCRLR
--------10--------20--------30--------40---

Chain assignments

Entity_assembly_ID (NMR)	Auth_asym_ID (model)	Length	Unmapped	Conflict	Sequence coverage (%)
A	A	43	0	0	100.0
B	B	43	0	0	100.0

Content subtype: combined_18763_2lzl.nef

#	Content subtype	Saveframe	Status	# of rows (sets)	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	484		100.0 (chain: A, length: 43)
1	Distance restraints	DYANA/DIANA_distance_constraints_2	Warning	23 (1)	noe	18.6 (chain: A, length: 43), 18.6 (chain: B, length: 43)

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1

Status: Warning

Sequence coverage of experimental data

Entity_assembly_ID: A, Chain length: 43, Sequence coverage: 100.0%

---360-----370-------380-------390---------
LPAEEELVEADEAGSVYAGILSYGVGFFLFILVVAAVTLCRLR
|||||||||||||||||||||||||||||||||||||||||||
LPAEEELVEADEAGSVYAGILSYGVGFFLFILVVAAVTLCRLR

Total number of assignments
- ¹H chemical shifts: 248
- ¹³C chemical shifts: 189
- ¹⁵N chemical shifts: 47

Completeness of assignments

Entity_assemble_ID: A

Excluded Atom_ID in statistics

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
397	ARG	HH11	6.886
397	ARG	HH12	6.886
397	ARG	HH21	6.908
397	ARG	HH22	6.908
397	ARG	CZ	159.35
397	ARG	NH1	72.241
397	ARG	NH2	72.244
399	ARG	HH11	6.814
399	ARG	HH12	6.814
399	ARG	HH21	6.835
399	ARG	HH22	6.835
399	ARG	CZ	159.267
399	ARG	NH1	72.266
399	ARG	NH2	72.302

All atoms

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	241	240	99.6
¹³C chemical shifts	199	187	94.0
¹⁵N chemical shifts	44	43	97.7

Backbone atoms

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	89	88	98.9
¹³C chemical shifts	86	84	97.7
¹⁵N chemical shifts	42	41	97.6

Side chain atoms

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	152	152	100.0
¹³C chemical shifts	113	103	91.2
¹⁵N chemical shifts	2	2	100.0

Methyl group atoms

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	37	37	100.0
¹³C chemical shifts	37	37	100.0

Aromatic group atoms

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	23	23	100.0
¹³C chemical shifts	23	13	56.5

Redox state of CYS
Peptide bond of PRO
Rotameric state of ILE , LEU, and VAL
Histogram of normalized assigned chemical shifts
Random coil index and derived parameters (S² and NMR RMSD)
- Chain_ID: A

Distance restraints

Saveframe: DYANA/DIANA_distance_constraints_2
- Status: Warning
- Experiment type: noe
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 43, Sequence coverage: 18.6%
  - Entity_assembly_ID: B, Chain length: 43, Sequence coverage: 18.6%
- Total number of restraints: 23
- Weight of restraints: 1.0 (23)
- Potential type of restraints: upper-bound-parabolic (23)
- Range of distance target values: 0.0, 4.3 (Å)
- Histogram of distance target values
- Distance restraints per residue
  - Chain_ID: A
  - Chain_ID: B
- Distance restraints on contact map

Keywords dimerization, FGFR3, FGFR3tm, fibroblast growth factor receptor 3, transmembrane domain