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Structural study of NS2(2-32) GBVB protein
Authors
Montserret, R., Penin, F., Martin, A.
Assembly
NS2(2-32) GBVB protein
Entity
1. NS2(2-32) GBVB protein (polymer, Thiol state: not present), 31 monomers, 3367.011 Da Detail

DTEIIGGLTI PPVVALVVMS RFGFFAHLLP R


Formula weight
3367.011 Da
Source organism
GB virus-B
Exptl. method
solution NMR
Refine. method
torsion angle dynamics
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 100.0 %, Completeness: 83.3 %, Completeness (bb): 80.3 % Detail

Polymer type: polypeptide(L)

Total1H13C
All83.3 % (280 of 336)94.1 % (174 of 185)70.2 % (106 of 151)
Backbone80.3 % (122 of 152)95.2 % (59 of 62)70.0 % (63 of 90)
Sidechain87.7 % (186 of 212)93.5 % (115 of 123)79.8 % (71 of 89)
Aromatic29.4 % (10 of 34)58.8 % (10 of 17) 0.0 % (0 of 17)
Methyl100.0 % (52 of 52)100.0 % (26 of 26)100.0 % (26 of 26)

1. NS2 2-32 GBVB

DTEIIGGLTI PPVVALVVMS RFGFFAHLLP R

Sample

Solvent system trifluoroethanol/water, Pressure 1 atm, Temperature 273 (±0.1) K, pH 6 (±1)


#NameIsotope labelingTypeConcentration
1NS2_2-32_GBVB[U-99% 2H]2 (±0.1) mM
2TFEnatural abundance50 % v/v
3H2Onatural abundance50 % v/v

Protein Blocks Logo
Calculated from 39 models in PDB: 2LZP, Strand ID: A Detail


Release date
2012-10-09
Citation
NS2 proteins of GB virus B and hepatitis C virus share common protease activities and membrane topologies
Boukadida, C., Marnata, C., Montserret, R., Cohen, L., Blumen, B., Gouttenoire, J., Moradpour, D., Penin, F., Martin, A.
J. Virol. (2014), 88, 7426-7444, PubMed 24741107 , DOI 10.1128/JVI.00656-14 ,
Related entities 1. NS2(2-32) GBVB protein, : 1 : 1 : 5 entities Detail
Experiments performed 3 experiments Detail
NMR combined restraints 3 contents Detail
Keywords GBVB, NS2, virus