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BMRB: 18776

2MPU

1H, 13C, 15N backbone NMR resonance assignments for N-terminal RNA recognition motif of HvRBP1 from Hordeum vulgare L. (barley)

Authors

Mason, K.E., Tripet, B.P., Fischer, A.M., Copie, V.

Assembly

Hordeum vulgare glycine rich- RNA binding protein 1

Entity

1. Hordeum vulgare glycine rich- RNA binding protein 1 (polymer, Thiol state: all free), 92 monomers, 9980.747 Da Detail

MAESDGAEYR CFVGSLSWNT DDRGLEAAFS SFGEILDAKI INDRETGRSR GFGFVSFSNE QAMQDAIEGM NGKELDGRSI VVNEAQSRGY GG

Formula weight

9980.747 Da

Source organism

Hordeum vulgare

Exptl. method

solution NMR

Refine. method

molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 76.0 %, Completeness (bb): 84.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	76.0 % (760 of 1000)	78.2 % (405 of 518)	68.8 % (262 of 381)	92.1 % (93 of 101)
Backbone	84.6 % (467 of 552)	97.5 % (192 of 197)	69.6 % (183 of 263)	100.0 % (92 of 92)
Sidechain	70.6 % (372 of 527)	66.4 % (213 of 321)	80.2 % (158 of 197)	11.1 % (1 of 9)
Aromatic	18.2 % (16 of 88)	18.2 % (8 of 44)	18.6 % (8 of 43)	0.0 % (0 of 1)
Methyl	87.5 % (63 of 72)	80.6 % (29 of 36)	94.4 % (34 of 36)

1. RBP1

MAESDGAEYR CFVGSLSWNT DDRGLEAAFS SFGEILDAKI INDRETGRSR GFGFVSFSNE QAMQDAIEGM NGKELDGRSI VVNEAQSRGY GG

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	RBP1	[U-99% 13C; U-99% 15N]	15 mM
2	sodium azide	natural abundance	0.01 %
3	potassium phosphate	natural abundance	50 mM
4	D2O	natural abundance	5 %
5	EDTA	natural abundance	1 mM
6	sodium chloride	natural abundance	500 mM
7	PMSF	natural abundance	1 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹⁵C	DSS	methyl carbons	external	indirect	0.1013291
¹H	DSS	methyl protons	internal	direct	1.0
¹³N	DSS	methyl carbons	external	indirect	0.2514495

Referencing offset: -0.59 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹⁵C	DSS	methyl carbons	external	indirect	0.1013291
¹H	DSS	methyl protons	internal	direct	1.0
¹³N	DSS	methyl carbons	external	indirect	0.2514495

Referencing offset: -0.59 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹⁵C	DSS	methyl carbons	external	indirect	0.1013291
¹H	DSS	methyl protons	internal	direct	1.0
¹³N	DSS	methyl carbons	external	indirect	0.2514495

Referencing offset: -0.01 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2MPU, Strand ID: A Detail

Release date

2013-01-21

Citation

¹H, ¹³C, ¹⁵N backbone and side chain NMR resonance assignments for the N-terminal RNA recognition motif of the HvGR-RBP1 protein involved in the regulation of barley (Hordeum vulgare L.) senescence
Mason, K.E., Tripet, B.P., Parrott, D., Fischer, A.M., Copie, V.
Biomol. NMR Assign. (2014), 8, 149-153, PubMed 23417794 , DOI 10.1007/s12104-013-9472-8 , Abstract

Related entities 1. Hordeum vulgare glycine rich- RNA binding protein 1, : 1 : 361 entities Detail

Experiments performed 10 experiments Detail

1 2D 1H-15N HSQC