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BMRB: 18782

2YMJ

Solution structure of the QUA1 dimerization domain of pXqua, the Xenopus ortholog of Quaking.

Authors

Ali, M., Broadhurst, R.WILLIAM.

Assembly

Solution structure of the QUA1 dimerization domain of pXqua, the Xenopus ortholog of Quaking.

Entity

1. Solution structure of the QUA1 dimerization domain of pXqua, the Xenopus ortholog of Quaking. (polymer, Thiol state: not present), 52 monomers, 6129.068 Da Detail

GSKEKPKPTP DYLMQLMNDK KLMSSLPNFS GIFTHLERLL DEEISRVRKD MY

Formula weight

6129.068 Da

Source organism

Exptl. method

Refine. method

ARIA VERSION 2.3

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 96.4 %, Completeness (bb): 96.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	96.4 % (623 of 646)	95.9 % (331 of 345)	97.2 % (243 of 250)	96.1 % (49 of 51)
Backbone	96.7 % (294 of 304)	96.1 % (98 of 102)	97.4 % (150 of 154)	95.8 % (46 of 48)
Sidechain	96.7 % (379 of 392)	95.9 % (233 of 243)	97.9 % (143 of 146)	100.0 % (3 of 3)
Aromatic	100.0 % (40 of 40)	100.0 % (20 of 20)	100.0 % (20 of 20)
Methyl	93.2 % (41 of 44)	95.5 % (21 of 22)	90.9 % (20 of 22)

1. PROTEIN QUAKING-A

GSKEKPKPTP DYLMQLMNDK KLMSSLPNFS GIFTHLERLL DEEISRVRKD MY

Show sample condition

Sample

Solvent system 90% water / 10% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 6.000, Details uniformly 15N/13C labelled pXqua QUA1 domain, 1.0 mmol/L

#	Name	Isotope labeling	Type	Concentration
1	PROTEIN QUAKING-A	[U-13C; U-15N]		1.0 mM

Protein Blocks Logo

Calculated from 20 models in PDB: 2YMJ, Strand ID: A Detail

Release date

2015-07-02

Citation

Solution structure of the QUA1 dimerization domain of pXqua, the Xenopus ortholog of Quaking
Ali, M., Broadhurst, R.WILLIAM.
PLoS One (2013), 8, e57345-e57345, PubMed 23520467 , DOI 10.1371/journal.pone.0057345 , Abstract

Related entities 1. Solution structure of the QUA1 dimerization domain of pXqua, the Xenopus ortholog of Quaking., : 1 : 30 entities Detail

Interaction partners 1. Solution structure of the QUA1 dimerization domain of pXqua, the Xenopus ortholog of Quaking., : 42 interactors Detail

More details for 42 interactors identified by 15 citations

Experiments performed 11 experiments Detail

1 [1H-15N]-HSQC

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% water / 10% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 6.000, Details uniformly 15N/13C labelled pXqua QUA1 domain, 1.0 mmol/L

#	Name	Isotope labeling	Type	Concentration
1	PROTEIN QUAKING-A	[U-13C; U-15N]		1.0 mM

2 15N-TOCSY-HSQC

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% water / 10% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 6.000, Details uniformly 15N/13C labelled pXqua QUA1 domain, 1.0 mmol/L

#	Name	Isotope labeling	Type	Concentration
1	PROTEIN QUAKING-A	[U-13C; U-15N]		1.0 mM

3 15N-NOESY-HSQC

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% water / 10% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 6.000, Details uniformly 15N/13C labelled pXqua QUA1 domain, 1.0 mmol/L

#	Name	Isotope labeling	Type	Concentration
1	PROTEIN QUAKING-A	[U-13C; U-15N]		1.0 mM

4 13C-NOESY-HSQC

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% water / 10% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 6.000, Details uniformly 15N/13C labelled pXqua QUA1 domain, 1.0 mmol/L

#	Name	Isotope labeling	Type	Concentration
1	PROTEIN QUAKING-A	[U-13C; U-15N]		1.0 mM

5 HNCA

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% water / 10% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 6.000, Details uniformly 15N/13C labelled pXqua QUA1 domain, 1.0 mmol/L

#	Name	Isotope labeling	Type	Concentration
1	PROTEIN QUAKING-A	[U-13C; U-15N]		1.0 mM

6 HN(CO)CA

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% water / 10% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 6.000, Details uniformly 15N/13C labelled pXqua QUA1 domain, 1.0 mmol/L

#	Name	Isotope labeling	Type	Concentration
1	PROTEIN QUAKING-A	[U-13C; U-15N]		1.0 mM

7 HNCACB

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% water / 10% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 6.000, Details uniformly 15N/13C labelled pXqua QUA1 domain, 1.0 mmol/L

#	Name	Isotope labeling	Type	Concentration
1	PROTEIN QUAKING-A	[U-13C; U-15N]		1.0 mM

8 CBCA(CO)NH

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% water / 10% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 6.000, Details uniformly 15N/13C labelled pXqua QUA1 domain, 1.0 mmol/L

#	Name	Isotope labeling	Type	Concentration
1	PROTEIN QUAKING-A	[U-13C; U-15N]		1.0 mM

9 HNCO

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% water / 10% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 6.000, Details uniformly 15N/13C labelled pXqua QUA1 domain, 1.0 mmol/L

#	Name	Isotope labeling	Type	Concentration
1	PROTEIN QUAKING-A	[U-13C; U-15N]		1.0 mM

10 HBHA(CO)NH

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% water / 10% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 6.000, Details uniformly 15N/13C labelled pXqua QUA1 domain, 1.0 mmol/L

#	Name	Isotope labeling	Type	Concentration
1	PROTEIN QUAKING-A	[U-13C; U-15N]		1.0 mM

11 HCCH-TOCSY

Instrument

Bruker Avance - 500 MHz

Sample

State isotropic, Solvent system 90% water / 10% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 6.000, Details uniformly 15N/13C labelled pXqua QUA1 domain, 1.0 mmol/L

#	Name	Isotope labeling	Type	Concentration
1	PROTEIN QUAKING-A	[U-13C; U-15N]		1.0 mM

nullKeywords NMR, QKI, Quaking, STAR protein, TRANSLATION, dimer, hairpin