BMRBj - BMREntryMaster

Found 1 Document (4.462 seconds)

BMRB: 18804

2M0B

Homodimeric transmembrane domain of the human receptor tyrosine kinase ErbB1 (EGFR, HER1) in micelles

Authors

Lesovoy, D.M., Bocharov, E.V., Pustovalova, Y.E., Bocharova, O.V., Arseniev, A.S.

Assembly

ErbB1 (EGFR, HER1)

Entity

1. ErbB1 (EGFR, HER1) (polymer), 44 monomers, 4725.781 × 2 Da Detail

EGCPTNGPKI PSIATGMVGA LLLLLVVALG IGLFMRRRHI VRKR

Total weight

9451.562 Da

Max. entity weight

4725.781 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing, TORSION ANGLE DYNAMICS

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 96.9 %, Completeness (bb): 97.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	96.9 % (496 of 512)	95.5 % (255 of 267)	98.5 % (200 of 203)	97.6 % (41 of 42)
Backbone	97.3 % (251 of 258)	95.6 % (87 of 91)	98.4 % (124 of 126)	97.6 % (40 of 41)
Sidechain	96.9 % (283 of 292)	95.5 % (168 of 176)	99.1 % (114 of 115)	100.0 % (1 of 1)
Aromatic	92.9 % (13 of 14)	100.0 % (7 of 7)	85.7 % (6 of 7)
Methyl	100.0 % (70 of 70)	100.0 % (35 of 35)	100.0 % (35 of 35)

1. ErbB1tm

EGCPTNGPKI PSIATGMVGA LLLLLVVALG IGLFMRRRHI VRKR

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 K, pH 5.0

#	Name	Isotope labeling	Concentration
1	ErbB1tm	[U-99% 13C; U-99% 15N]	0.75 mM
2	ErbB1tm	natural abundance	0.75 mM
3	DPC	[U-99% 2H]	90 mM
4	sodium azide	natural abundance	0.3 mM
5	TCEP	natural abundance	6 mM
6	citric acid	natural abundance	10 mM
7	Na2HPO4	natural abundance	20 mM

Sample #2

Solvent system 99.9% D2O, Pressure 1 atm, Temperature 313 K, pH 5.0

#	Name	Isotope labeling	Concentration
8	ErbB1tm	[U-99% 13C; U-99% 15N]	0.75 mM
9	ErbB1tm	natural abundance	0.75 mM
10	DPC	[U-99% 2H]	90 mM
11	sodium azide	natural abundance	0.3 mM
12	TCEP	natural abundance	6 mM
13	citric acid	natural abundance	10 mM
14	Na2HPO4	natural abundance	20 mM

Protein Blocks Logo

Calculated from 20 models in PDB: 2M0B, Strand ID: A, B Detail

Release date

2013-10-21

Related entities 1. ErbB1 (EGFR, HER1), : 1 : 4 : 2 : 25 entities Detail

Interaction partners 1. ErbB1 (EGFR, HER1), : 293 interactors Detail

More details for 293 interactors identified by 80 citations

Experiments performed 16 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 K, pH 5.0

#	Name	Isotope labeling	Concentration
1	ErbB1tm	[U-99% 13C; U-99% 15N]	0.75 mM
2	ErbB1tm	natural abundance	0.75 mM
3	DPC	[U-99% 2H]	90 mM
4	sodium azide	natural abundance	0.3 mM
5	TCEP	natural abundance	6 mM
6	citric acid	natural abundance	10 mM
7	Na2HPO4	natural abundance	20 mM

2 2D 1H-15N TROSY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 K, pH 5.0

#	Name	Isotope labeling	Concentration
1	ErbB1tm	[U-99% 13C; U-99% 15N]	0.75 mM
2	ErbB1tm	natural abundance	0.75 mM
3	DPC	[U-99% 2H]	90 mM
4	sodium azide	natural abundance	0.3 mM
5	TCEP	natural abundance	6 mM
6	citric acid	natural abundance	10 mM
7	Na2HPO4	natural abundance	20 mM

3 2D 1H-13C constant time HSQC aliphatic

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 99.9% D2O, Pressure 1 atm, Temperature 313 K, pH 5.0

#	Name	Isotope labeling	Concentration
8	ErbB1tm	[U-99% 13C; U-99% 15N]	0.75 mM
9	ErbB1tm	natural abundance	0.75 mM
10	DPC	[U-99% 2H]	90 mM
11	sodium azide	natural abundance	0.3 mM
12	TCEP	natural abundance	6 mM
13	citric acid	natural abundance	10 mM
14	Na2HPO4	natural abundance	20 mM

4 2D 1H-13C constant time HSQC aromatic

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 99.9% D2O, Pressure 1 atm, Temperature 313 K, pH 5.0

#	Name	Isotope labeling	Concentration
8	ErbB1tm	[U-99% 13C; U-99% 15N]	0.75 mM
9	ErbB1tm	natural abundance	0.75 mM
10	DPC	[U-99% 2H]	90 mM
11	sodium azide	natural abundance	0.3 mM
12	TCEP	natural abundance	6 mM
13	citric acid	natural abundance	10 mM
14	Na2HPO4	natural abundance	20 mM

5 3D HNCO

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 K, pH 5.0

#	Name	Isotope labeling	Concentration
1	ErbB1tm	[U-99% 13C; U-99% 15N]	0.75 mM
2	ErbB1tm	natural abundance	0.75 mM
3	DPC	[U-99% 2H]	90 mM
4	sodium azide	natural abundance	0.3 mM
5	TCEP	natural abundance	6 mM
6	citric acid	natural abundance	10 mM
7	Na2HPO4	natural abundance	20 mM

6 3D HNCA

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 K, pH 5.0

#	Name	Isotope labeling	Concentration
1	ErbB1tm	[U-99% 13C; U-99% 15N]	0.75 mM
2	ErbB1tm	natural abundance	0.75 mM
3	DPC	[U-99% 2H]	90 mM
4	sodium azide	natural abundance	0.3 mM
5	TCEP	natural abundance	6 mM
6	citric acid	natural abundance	10 mM
7	Na2HPO4	natural abundance	20 mM

7 3D HN(CO)CA

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 K, pH 5.0

#	Name	Isotope labeling	Concentration
1	ErbB1tm	[U-99% 13C; U-99% 15N]	0.75 mM
2	ErbB1tm	natural abundance	0.75 mM
3	DPC	[U-99% 2H]	90 mM
4	sodium azide	natural abundance	0.3 mM
5	TCEP	natural abundance	6 mM
6	citric acid	natural abundance	10 mM
7	Na2HPO4	natural abundance	20 mM

8 3D HN(CA)CO

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 K, pH 5.0

#	Name	Isotope labeling	Concentration
1	ErbB1tm	[U-99% 13C; U-99% 15N]	0.75 mM
2	ErbB1tm	natural abundance	0.75 mM
3	DPC	[U-99% 2H]	90 mM
4	sodium azide	natural abundance	0.3 mM
5	TCEP	natural abundance	6 mM
6	citric acid	natural abundance	10 mM
7	Na2HPO4	natural abundance	20 mM

9 3D HNHA

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 K, pH 5.0

#	Name	Isotope labeling	Concentration
1	ErbB1tm	[U-99% 13C; U-99% 15N]	0.75 mM
2	ErbB1tm	natural abundance	0.75 mM
3	DPC	[U-99% 2H]	90 mM
4	sodium azide	natural abundance	0.3 mM
5	TCEP	natural abundance	6 mM
6	citric acid	natural abundance	10 mM
7	Na2HPO4	natural abundance	20 mM

10 3D HNHB

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 K, pH 5.0

#	Name	Isotope labeling	Concentration
1	ErbB1tm	[U-99% 13C; U-99% 15N]	0.75 mM
2	ErbB1tm	natural abundance	0.75 mM
3	DPC	[U-99% 2H]	90 mM
4	sodium azide	natural abundance	0.3 mM
5	TCEP	natural abundance	6 mM
6	citric acid	natural abundance	10 mM
7	Na2HPO4	natural abundance	20 mM

11 3D HCCH-TOCSY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 K, pH 5.0

#	Name	Isotope labeling	Concentration
1	ErbB1tm	[U-99% 13C; U-99% 15N]	0.75 mM
2	ErbB1tm	natural abundance	0.75 mM
3	DPC	[U-99% 2H]	90 mM
4	sodium azide	natural abundance	0.3 mM
5	TCEP	natural abundance	6 mM
6	citric acid	natural abundance	10 mM
7	Na2HPO4	natural abundance	20 mM

12 3D 1H-15N(TROSY) NOESY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 K, pH 5.0

#	Name	Isotope labeling	Concentration
1	ErbB1tm	[U-99% 13C; U-99% 15N]	0.75 mM
2	ErbB1tm	natural abundance	0.75 mM
3	DPC	[U-99% 2H]	90 mM
4	sodium azide	natural abundance	0.3 mM
5	TCEP	natural abundance	6 mM
6	citric acid	natural abundance	10 mM
7	Na2HPO4	natural abundance	20 mM

13 3D 1H-13C NOESY aliphatic

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 K, pH 5.0

#	Name	Isotope labeling	Concentration
1	ErbB1tm	[U-99% 13C; U-99% 15N]	0.75 mM
2	ErbB1tm	natural abundance	0.75 mM
3	DPC	[U-99% 2H]	90 mM
4	sodium azide	natural abundance	0.3 mM
5	TCEP	natural abundance	6 mM
6	citric acid	natural abundance	10 mM
7	Na2HPO4	natural abundance	20 mM

14 3D 1H-13C(constant time) NOESY aliphatic

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 K, pH 5.0

#	Name	Isotope labeling	Concentration
1	ErbB1tm	[U-99% 13C; U-99% 15N]	0.75 mM
2	ErbB1tm	natural abundance	0.75 mM
3	DPC	[U-99% 2H]	90 mM
4	sodium azide	natural abundance	0.3 mM
5	TCEP	natural abundance	6 mM
6	citric acid	natural abundance	10 mM
7	Na2HPO4	natural abundance	20 mM

15 15N,13C-F1-filtered/F3-edited-NOESY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 K, pH 5.0

#	Name	Isotope labeling	Concentration
1	ErbB1tm	[U-99% 13C; U-99% 15N]	0.75 mM
2	ErbB1tm	natural abundance	0.75 mM
3	DPC	[U-99% 2H]	90 mM
4	sodium azide	natural abundance	0.3 mM
5	TCEP	natural abundance	6 mM
6	citric acid	natural abundance	10 mM
7	Na2HPO4	natural abundance	20 mM

16 15N,13C-F1-filtered/F3-edited-NOESY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 99.9% D2O, Pressure 1 atm, Temperature 313 K, pH 5.0

#	Name	Isotope labeling	Concentration
8	ErbB1tm	[U-99% 13C; U-99% 15N]	0.75 mM
9	ErbB1tm	natural abundance	0.75 mM
10	DPC	[U-99% 2H]	90 mM
11	sodium azide	natural abundance	0.3 mM
12	TCEP	natural abundance	6 mM
13	citric acid	natural abundance	10 mM
14	Na2HPO4	natural abundance	20 mM

nullKeywords dimer, ErbB1, homodimer, human, receptor, transmembrane domain, tyrosine kinase

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Found 1 Document (4.462 seconds)

BMRB: 18804

Sample #1

Sample #2

Related entities 1. ErbB1 (EGFR, HER1), : 1 : 4 : 2 : 25 entities Detail

Interaction partners 1. ErbB1 (EGFR, HER1), : 293 interactors Detail

Experiments performed 16 experiments Detail

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