BMRBj - BMREntryMaster

Found 1 Document (0.981 seconds)

BMRB: 18806

2M0D

Solution Structure of Miz-1 zinc fingers 5 to 7

Authors

Bernard, D., Bedard, M., Bilodeau, J., Lavigne, P.

Assembly

Miz58

Entity

1. Miz58 (polymer, Thiol state: all free), 112 monomers, 12933.70 Da Detail

MKPYQCDYCG RSFSDPTSKM RHLETHDTDK EHKCPHCDKK FNQVGNLKAH LKIHIADGPL KCRECGKQFT TSGNLKRHLR IHSGEKPYVC IHCQRQFADP GALQRHVRIH TG

2. ZINC ION (non-polymer, Thiol state: not present), 65.409 Da

Total weight

12999.109 Da

Max. entity weight

12933.7 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.4 %, Completeness: 87.3 %, Completeness (bb): 92.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	87.3 % (1158 of 1326)	86.6 % (609 of 703)	87.8 % (446 of 508)	89.6 % (103 of 115)
Backbone	92.3 % (609 of 660)	91.6 % (207 of 226)	92.1 % (302 of 328)	94.3 % (100 of 106)
Sidechain	84.0 % (647 of 770)	84.3 % (402 of 477)	85.2 % (242 of 284)	33.3 % (3 of 9)
Aromatic	70.4 % (76 of 108)	77.8 % (42 of 54)	63.0 % (34 of 54)
Methyl	93.8 % (75 of 80)	97.5 % (39 of 40)	90.0 % (36 of 40)

1. Miz58

MKPYQCDYCG RSFSDPTSKM RHLETHDTDK EHKCPHCDKK FNQVGNLKAH LKIHIADGPL KCRECGKQFT TSGNLKRHLR IHSGEKPYVC IHCQRQFADP GALQRHVRIH TG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	Miz58	[U-13C; U-15N]	0.65 mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.13 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.13 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.15 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.06 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2M0D, Strand ID: A Detail

Release date

2012-12-06

Citation

Structural and dynamical characterization of the Miz-1 zinc fingers 5-8 by solution-state NMR
Bernard, D., Bedard, M., Bilodeau, J., Lavigne, P.
J. Biomol. NMR (2013), 57, 103-116, PubMed 23975355 , DOI 10.1007/s10858-013-9770-6 , Abstract

Related entities 1. Miz58, : 1 : 2 : 5 : 1778 entities Detail

Interaction partners 1. Miz58, : 21 interactors Detail

More details for 21 interactors identified by 12 citations

Experiments performed 11 experiments Detail

1 3D HNCACB