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BMRB: 18817

2M0Q

Solution NMR analysis of intact KCNE2 in detergent micelles demonstrate a straight transmembrane helix

Authors

Lai, C., Li, P., Chen, L., Zhang, L., Wu, F., Tian, C.

Assembly

KCNE2

Entity

1. KCNE2 (polymer, Thiol state: not present), 123 monomers, 14471.36 Da Detail

MSTLSNFTQT LEDVFRRIFI TYMDNWRQNT TAEQEALQAK VDAENFYYVI LYLMVMIGMF SFIIVAILVS TVKSKRREHS NDPYHQYIVE DWQEKYKSQI LNLEESKATI HENIGAAGFK MSP

Formula weight

14471.36 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.3 %, Completeness: 41.2 %, Completeness (bb): 77.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	41.2 % (621 of 1507)	22.3 % (174 of 780)	58.0 % (342 of 590)	76.6 % (105 of 137)
Backbone	77.4 % (568 of 734)	51.0 % (126 of 247)	92.3 % (338 of 366)	86.0 % (104 of 121)
Sidechain	18.4 % (164 of 893)	9.0 % (48 of 533)	33.4 % (115 of 344)	6.3 % (1 of 16)
Aromatic	1.2 % (2 of 162)	2.5 % (2 of 81)	0.0 % (0 of 79)	0.0 % (0 of 2)
Methyl	8.6 % (12 of 140)	7.1 % (5 of 70)	10.0 % (7 of 70)

1. KCNE2

MSTLSNFTQT LEDVFRRIFI TYMDNWRQNT TAEQEALQAK VDAENFYYVI LYLMVMIGMF SFIIVAILVS TVKSKRREHS NDPYHQYIVE DWQEKYKSQI LNLEESKATI HENIGAAGFK MSP

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	KCNE2	[U-15N]	1.0 mM
2	H2O	natural abundance	90 %
3	D2O	[U-2H]	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
4	KCNE2	[U-13C; U-15N]	1.0 mM
5	H2O	natural abundance	90 %
6	D2O	[U-2H]	10 %

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
7	KCNE2	[U-13C; U-15N; U-2H]	1.0 mM
8	H2O	natural abundance	90 %
9	D2O	[U-2H]	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.43 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.43 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.41 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 2M0Q, Strand ID: A Detail

Release date

2014-04-27

Citation

Differential modulations of KCNQ1 by auxiliary proteins KCNE1 and KCNE2
Li, P., Liu, H., Lai, C., Sun, P., Zeng, W., Wu, F., Zhang, L., Wang, S., Tian, C., Ding, J.
Sci. Rep. (2014), 4, 4973-4973, PubMed 24827085 , DOI 10.1038/srep04973 , Abstract

Related entities 1. KCNE2, : 1 : 2 : 4 entities Detail

Experiments performed 10 experiments Detail

1 2D 1H-15N HSQC