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BMRB: 18840

2M13

The ZZ domain of cytoplasmic polyadenylation element binding protein 1 (CPEB1)

Authors

Lee, B.M., Merkel, D.J., Wells, S.B., Hilburn, B.C., Elazzouzi, F., Perez-Alvarado, G.C.

Assembly

CPEB1-ZZ G-P-504-566

Entity

1. CPEB1-ZZ G-P-504-566 (polymer, Thiol state: all other bound), 65 monomers, 7755.734 Da Detail

GPVQIDPYLE DSLCHICSSQ PGPFFCRDQV CFKYFCRSCW HWRHSMEGLR HHSPLMRNQK NRDSS

2. ZINC ION (non-polymer, Thiol state: not present), 65.409 × 2 Da

Total weight

7886.552 Da

Max. entity weight

7755.734 Da

Entity Connection

na 8 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS14:SG	2:ZN1:ZN
2	na	sing	1:CYS17:SG	2:ZN1:ZN
3	na	sing	1:CYS36:SG	2:ZN1:ZN
4	na	sing	1:CYS39:SG	2:ZN1:ZN
5	na	sing	1:CYS26:SG	2:ZN1:ZN
6	na	sing	1:CYS31:SG	2:ZN1:ZN
7	na	sing	1:HIS44:NE2	2:ZN1:ZN
8	na	sing	1:HIS52:ND1	2:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

molecular dynamics, torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 95.0 %, Completeness (bb): 96.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	95.0 % (743 of 782)	95.4 % (395 of 414)	94.0 % (282 of 300)	97.1 % (66 of 68)
Backbone	96.3 % (366 of 380)	97.7 % (125 of 128)	95.3 % (183 of 192)	96.7 % (58 of 60)
Sidechain	94.6 % (439 of 464)	94.4 % (270 of 286)	94.7 % (161 of 170)	100.0 % (8 of 8)
Aromatic	84.0 % (84 of 100)	84.0 % (42 of 50)	83.3 % (40 of 48)	100.0 % (2 of 2)
Methyl	100.0 % (32 of 32)	100.0 % (16 of 16)	100.0 % (16 of 16)

1. CPEB1-ZZ G-P-504-566

GPVQIDPYLE DSLCHICSSQ PGPFFCRDQV CFKYFCRSCW HWRHSMEGLR HHSPLMRNQK NRDSS

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	CPEB1-ZZ G-P-504-566	[U-100% 13C; U-100% 15N]	0.3 mM
2	TRIS	natural abundance	20 mM
3	sodium chloride	natural abundance	200 mM
4	DTT	natural abundance	1 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 7.0

#	Name	Isotope labeling	Concentration
7	CPEB1-ZZ T498S-566	[U-100% 13C; U-100% 15N]	0.3 mM
8	potassium phosphate	natural abundance	50 mM
9	potassium chloride	natural abundance	200 mM
10	DTT	natural abundance	5 mM
11	H2O	natural abundance	90 %
12	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: -0.22 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: -0.22 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: 0.03 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: 0.15 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2M13, Strand ID: A Detail

Release date

2013-03-31

Citation

The C-terminal region of cytoplasmic polyadenylation element binding protein is a ZZ domain with potential for protein-protein interactions
Merkel, D.J., Wells, S.B., Hilburn, B.C., Elazzouzi, F., Perez-Alvarado, G.C., Lee, B.M.
J. Mol. Biol. (2013), 425, 2015-2026, PubMed 23500490 , DOI 10.1016/j.jmb.2013.03.009 , Abstract

Related entities 1. CPEB1-ZZ G-P-504-566, : 1 : 8 : 45 entities Detail

Interaction partners 1. CPEB1-ZZ G-P-504-566, : 4 interactors Detail

More details for 4 interactors identified by 2 citations

Experiments performed 23 experiments Detail

1 2D 1H-15N HSQC