BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	98.9 % (1099 of 1111)	98.4 % (570 of 579)	99.8 % (430 of 431)	98.0 % (99 of 101)
Backbone	99.1 % (569 of 574)	99.0 % (197 of 199)	99.6 % (281 of 282)	97.8 % (91 of 93)
Sidechain	98.9 % (618 of 625)	98.2 % (373 of 380)	100.0 % (237 of 237)	100.0 % (8 of 8)
Aromatic	100.0 % (90 of 90)	100.0 % (45 of 45)	100.0 % (45 of 45)
Methyl	100.0 % (92 of 92)	100.0 % (46 of 46)	100.0 % (46 of 46)

1. SUP-12

GAMGSRDTMF TKIFVGGLPY HTSDKTLHEY FEQFGDIEEA VVITDRNTQK SRGYGFVTMK DRASAERACK DPNPIIDGRK ANVNLAYLGA KPRTNVQ

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	SUP-12	[U-99% 15N]	0.8 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	300 mM
4	H2O	natural abundance	90 %
5	D2O	natural abundance	10 %

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
6	SUP-12	[U-99% 15N]	0.8 mM
7	sodium phosphate	natural abundance	20 mM
8	sodium chloride	natural abundance	300 mM
9	D2O	natural abundance	100 %

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
10	SUP-12	[U-99% 13C; U-99% 15N]	0.8 mM
11	sodium phosphate	natural abundance	20 mM
12	sodium chloride	natural abundance	300 mM
13	H2O	natural abundance	90 %
14	D2O	natural abundance	10 %

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
15	SUP-12	[U-10% 13C; U-99% 15N]	0.8 mM
16	sodium phosphate	natural abundance	20 mM
17	sodium chloride	natural abundance	300 mM
18	H2O	natural abundance	90 %
19	D2O	natural abundance	10 %

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
20	SUP-12	[U-99% 13C; U-99% 15N; U-99% 2H; U-99% 1H ILV]	0.4 mM
21	sodium phosphate	natural abundance	20 mM
22	sodium chloride	natural abundance	300 mM
23	D2O	natural abundance	100 %

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
24	SUP-12	[U-99% 13C; U-99% 15N]	0.8 mM
25	sodium phosphate	natural abundance	20 mM
26	sodium chloride	natural abundance	300 mM
27	D2O	natural abundance	100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
¹H	water	protons	4.802 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Referencing offset: -0.16 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
¹H	water	protons	4.802 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Referencing offset: -0.16 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
¹H	water	protons	4.802 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Referencing offset: -0.02 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
¹H	water	protons	4.802 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Referencing offset: 0.44 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 15 models in PDB: 4CH0, Strand ID: S Detail

Release date

2013-01-28

Citation

Protein chemical shift assignments of the unbound and RNA-bound forms of the alternative splicing factor SUP-12 from C. elegans
Amrane, S., Mackereth, C.D.
Biomol. NMR Assign. (2014), 8, 109-112, PubMed 23334698 , DOI 10.1007/s12104-013-9463-9 , Abstract