Search

SUP-12
Authors
Amrane, S., Mackereth, C.D.
Assembly
SUP-12
Entity
1. SUP-12 (polymer, Thiol state: all free), 97 monomers, 10815.06 Da Detail

GAMGSRDTMF TKIFVGGLPY HTSDKTLHEY FEQFGDIEEA VVITDRNTQK SRGYGFVTMK DRASAERACK DPNPIIDGRK ANVNLAYLGA KPRTNVQ


Formula weight
10815.06 Da
Source organism
Caenorhabditis elegans
Exptl. method
solution NMR
Refine. method
ARIA1.2
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 100.0 %, Completeness: 98.9 %, Completeness (bb): 99.1 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All98.9 % (1099 of 1111)98.4 % (570 of 579)99.8 % (430 of 431)98.0 % (99 of 101)
Backbone99.1 % (569 of 574)99.0 % (197 of 199)99.6 % (281 of 282)97.8 % (91 of 93)
Sidechain98.9 % (618 of 625)98.2 % (373 of 380)100.0 % (237 of 237)100.0 % (8 of 8)
Aromatic100.0 % (90 of 90)100.0 % (45 of 45)100.0 % (45 of 45)
Methyl100.0 % (92 of 92)100.0 % (46 of 46)100.0 % (46 of 46)

1. SUP-12

GAMGSRDTMF TKIFVGGLPY HTSDKTLHEY FEQFGDIEEA VVITDRNTQK SRGYGFVTMK DRASAERACK DPNPIIDGRK ANVNLAYLGA KPRTNVQ

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5


#NameIsotope labelingTypeConcentration
1SUP-12[U-99% 15N]0.8 mM
2sodium phosphatenatural abundance20 mM
3sodium chloridenatural abundance300 mM
4H2Onatural abundance90 %
5D2Onatural abundance10 %
Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5


#NameIsotope labelingTypeConcentration
6SUP-12[U-99% 15N]0.8 mM
7sodium phosphatenatural abundance20 mM
8sodium chloridenatural abundance300 mM
9D2Onatural abundance100 %
Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5


#NameIsotope labelingTypeConcentration
10SUP-12[U-99% 13C; U-99% 15N]0.8 mM
11sodium phosphatenatural abundance20 mM
12sodium chloridenatural abundance300 mM
13H2Onatural abundance90 %
14D2Onatural abundance10 %
Sample #4

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5


#NameIsotope labelingTypeConcentration
15SUP-12[U-10% 13C; U-99% 15N]0.8 mM
16sodium phosphatenatural abundance20 mM
17sodium chloridenatural abundance300 mM
18H2Onatural abundance90 %
19D2Onatural abundance10 %
Sample #5

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5


#NameIsotope labelingTypeConcentration
20SUP-12[U-99% 13C; U-99% 15N; U-99% 2H; U-99% 1H ILV]0.4 mM
21sodium phosphatenatural abundance20 mM
22sodium chloridenatural abundance300 mM
23D2Onatural abundance100 %
Sample #6

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5


#NameIsotope labelingTypeConcentration
24SUP-12[U-99% 13C; U-99% 15N]0.8 mM
25sodium phosphatenatural abundance20 mM
26sodium chloridenatural abundance300 mM
27D2Onatural abundance100 %

LACS Plot; CA
Referencing offset: -0.16 ppm, Outliers: 1 Detail
LACS Plot; CB
Referencing offset: -0.16 ppm, Outliers: 1 Detail
LACS Plot; HA
Referencing offset: -0.02 ppm, Outliers: 1 Detail
LACS Plot; CO
Referencing offset: 0.44 ppm, Outliers: 1 Detail
Protein Blocks Logo
Calculated from 15 models in PDB: 4CH0, Strand ID: S Detail


Release date
2013-01-28
Citation
Protein chemical shift assignments of the unbound and RNA-bound forms of the alternative splicing factor SUP-12 from C. elegans
Amrane, S., Mackereth, C.D.
Biomol. NMR Assign. (2014), 8, 109-112, PubMed 23334698 , DOI 10.1007/s12104-013-9463-9 ,
Related entities 1. SUP-12, : 1 : 2 : 149 entities Detail
Interaction partners 1. SUP-12, : 5 interactors Detail
Experiments performed 16 experiments Detail
nullKeywords alternative splicing, muscle, nematode, RNA, RRM