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BMRB: 18846

4CIO

SUP-12 + GGUGUGC

Authors

Amrane, S., Mackereth, C.D.

Assembly

SUP-12

Entity

1. SUP-12 (polymer, Thiol state: all free), 97 monomers, 10815.06 Da Detail

GAMGSRDTMF TKIFVGGLPY HTSDKTLHEY FEQFGDIEEA VVITDRNTQK SRGYGFVTMK DRASAERACK DPNPIIDGRK ANVNLAYLGA KPRTNVQ

2. GGUGUGC (polymer, Thiol state: not present), 7 monomers, 2316.351 Da Detail

GGUGUGC

Total weight

13131.41 Da

Max. entity weight

10815.06 Da

Source organism

Caenorhabditis elegans

Exptl. method

solution NMR

Refine. method

ARIA1.2

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 93.3 %, Completeness: 89.6 %, Completeness (bb): 87.4 % Detail

Polymer type: polypeptide(L) polyribonucleotide

	Total	¹H	¹³C	¹⁵N
All	89.6 % (1095 of 1222)	88.4 % (565 of 639)	90.3 % (430 of 476)	93.5 % (100 of 107)
Backbone	87.4 % (569 of 651)	81.3 % (196 of 241)	88.6 % (281 of 317)	98.9 % (92 of 93)
Sidechain	93.2 % (614 of 659)	92.7 % (369 of 398)	96.0 % (237 of 247)	57.1 % (8 of 14)
Aromatic	73.8 % (90 of 122)	73.8 % (45 of 61)	81.8 % (45 of 55)	0.0 % (0 of 6)
Methyl	100.0 % (92 of 92)	100.0 % (46 of 46)	100.0 % (46 of 46)

1. SUP-12

GAMGSRDTMF TKIFVGGLPY HTSDKTLHEY FEQFGDIEEA VVITDRNTQK SRGYGFVTMK DRASAERACK DPNPIIDGRK ANVNLAYLGA KPRTNVQ

2. GGUGUGC

GGUGUGC

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	SUP-12	[U-99% 13C; U-99% 15N]	0.2 mM
2	GGUGUGC	natural abundance	0.24 mM
3	sodium phosphate	natural abundance	20 mM
4	sodium chloride	natural abundance	300 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
7	SUP-12	[U-99% 13C; U-99% 15N]	0.4 mM
8	GGUGUGC	natural abundance	0.42 mM
9	sodium phosphate	natural abundance	20 mM
10	sodium chloride	natural abundance	300 mM
11	D2O	natural abundance	100 %

Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
12	SUP-12	[U-10% 13C; U-99% 15N]	50 uM
13	GGUGUGC	natural abundance	75 uM
14	sodium phosphate	natural abundance	20 mM
15	sodium chloride	natural abundance	300 mM
16	D2O	natural abundance	100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
¹H	water	protons	4.802 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Referencing offset: -0.24 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
¹H	water	protons	4.802 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Referencing offset: -0.24 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
¹H	water	protons	4.802 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Referencing offset: 0.0 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
¹H	water	protons	4.802 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Referencing offset: 0.49 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 15 models in PDB: 4CIO, Strand ID: A Detail

Release date

2013-01-28

Citation

Protein chemical shift assignments of the unbound and RNA-bound forms of the alternative splicing factor SUP-12 from C. elegans
Amrane, S., Mackereth, C.D.
Biomol. NMR Assign. (2014), 8, 109-112, PubMed 23334698 , DOI 10.1007/s12104-013-9463-9 , Abstract

Related entities 1. SUP-12, : 1 : 2 : 149 entities Detail

Experiments performed 14 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 700 MHz 5 mm PATXI 1H-13C/15N/D Z-GRD

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	SUP-12	[U-99% 13C; U-99% 15N]	0.2 mM
2	GGUGUGC	natural abundance	0.24 mM
3	sodium phosphate	natural abundance	20 mM
4	sodium chloride	natural abundance	300 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

2 3D HNCO

Instrument

Bruker Avance - 700 MHz 5 mm PATXI 1H-13C/15N/D Z-GRD

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	SUP-12	[U-99% 13C; U-99% 15N]	0.2 mM
2	GGUGUGC	natural abundance	0.24 mM
3	sodium phosphate	natural abundance	20 mM
4	sodium chloride	natural abundance	300 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

3 3D HNCA

Instrument

Bruker Avance - 700 MHz 5 mm PATXI 1H-13C/15N/D Z-GRD

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	SUP-12	[U-99% 13C; U-99% 15N]	0.2 mM
2	GGUGUGC	natural abundance	0.24 mM
3	sodium phosphate	natural abundance	20 mM
4	sodium chloride	natural abundance	300 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

4 3D HNCACB

Instrument

Bruker Avance - 700 MHz 5 mm PATXI 1H-13C/15N/D Z-GRD

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	SUP-12	[U-99% 13C; U-99% 15N]	0.2 mM
2	GGUGUGC	natural abundance	0.24 mM
3	sodium phosphate	natural abundance	20 mM
4	sodium chloride	natural abundance	300 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

5 3D CBCA(CO)NH

Instrument

Bruker Avance - 700 MHz 5 mm PATXI 1H-13C/15N/D Z-GRD

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	SUP-12	[U-99% 13C; U-99% 15N]	0.2 mM
2	GGUGUGC	natural abundance	0.24 mM
3	sodium phosphate	natural abundance	20 mM
4	sodium chloride	natural abundance	300 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

6 2D 1H-13C HSQC aliphatic