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BMRB: 18870

2M1M

Solution structure of the dimerization domain of Aux/IAA transcription factor Ps-IAA4 from pea (Pisum sativum)

Authors

Kovermann, M., Dhurvas Chandrasekaran, D., Gopalswamy, M., Abel, S., Balbach, J.

Assembly

dimerization domain of Aux/IAA transcription factor Ps-IAA4 from pea (Pisum sativum)

Entity

1. dimerization domain of Aux/IAA transcription factor Ps-IAA4 from pea (Pisum sativum) (polymer), 107 monomers, 11922.50 Da Detail

HEADVGGIFV KVSMDGAPYL RKIDLRVYGG YSELLKALET MFKLTIGEYS EREGYKGSEY APTYEDKDGD WMLVGDVPWD MFVTSCKRLR IMKGTEAKGL GCGVGSH

Formula weight

11922.5 Da

Source organism

Pisum sativum

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.1 %, Completeness: 82.4 %, Completeness (bb): 92.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	82.4 % (1010 of 1226)	80.8 % (513 of 635)	81.6 % (396 of 485)	95.3 % (101 of 106)
Backbone	92.0 % (585 of 636)	91.6 % (207 of 226)	91.2 % (279 of 306)	95.2 % (99 of 104)
Sidechain	74.5 % (508 of 682)	74.8 % (306 of 409)	73.8 % (200 of 271)	100.0 % (2 of 2)
Aromatic	35.6 % (42 of 118)	42.4 % (25 of 59)	26.3 % (15 of 57)	100.0 % (2 of 2)
Methyl	96.2 % (100 of 104)	96.2 % (50 of 52)	96.2 % (50 of 52)

1. PsIAA4 DIII/IV

HEADVGGIFV KVSMDGAPYL RKIDLRVYGG YSELLKALET MFKLTIGEYS EREGYKGSEY APTYEDKDGD WMLVGDVPWD MFVTSCKRLR IMKGTEAKGL GCGVGSH

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 2.5, Details 13C 15N PsIAA4 DIII/IV

#	Name	Isotope labeling	Concentration
1	PsIAA4_DIII/IV	[U-98% 13C; U-98% 15N]	1 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	100 mM
4	magnesium chloride	natural abundance	2 mM
5	DTT	natural abundance	1 mM
6	D2O	natural abundance	10 %
7	H2O	natural abundance	90 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.12 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.12 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.08 ppm, Outliers: 4 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.27 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 2M1M, Strand ID: A Detail

Release date

2013-12-01

Citation

Solution structure of the dimerization domain of Aux/IAA transcription factor Ps-IAA4 from pea (Pisum sativum)
Dhurvas Chandrasekaran, D., Kovermann, M., Gopalswamy, M., Balbach, J., Abel, S.
Not known

Related entities 1. dimerization domain of Aux/IAA transcription factor Ps-IAA4 from pea (Pisum sativum), : 1 : 1 : 191 entities Detail

Interaction partners 1. dimerization domain of Aux/IAA transcription factor Ps-IAA4 from pea (Pisum sativum), : 2 interactors Detail

More details for 2 interactors identified by 1 citations

Experiments performed 12 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 600 MHz

Sample

State anisotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 2.5, Details 13C 15N PsIAA4 DIII/IV

#	Name	Isotope labeling	Concentration
1	PsIAA4_DIII/IV	[U-98% 13C; U-98% 15N]	1 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	100 mM
4	magnesium chloride	natural abundance	2 mM
5	DTT	natural abundance	1 mM
6	D2O	natural abundance	10 %
7	H2O	natural abundance	90 %

2 2D 1H-13C HSQC aliphatic